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HIGFMAG-52K MILLIPLEX MAP Human IGF-I, II Magnetic Bead Panel - Endocrine Assay

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HIGFMAG-52K
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Overview

Replacement Information

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
IGF-I IGF-II HMplexLuminex xMAP
Description
Catalogue NumberHIGFMAG-52K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human IGF-I, II Magnetic Bead Panel - Endocrine Assay
OverviewThis Human IGF-I, II Magnetic Bead Panel is a configurable two analyte panel for the quantitative determination of IGF-I and/or IGF-II in human serum, plasma, tissue/cell extracts, or culture media samples.
NOTE: Reagents are included for the extraction of serum and plasma samples resulting in a 1:60 final dilution.
Background InformationInsulin-like Growth Factors I and II are polypeptide, endocrine hormones similar in molecular structure to insulin. Both IGF-I and IGF-II are produced primarily by the liver and their bio-availabilities are regulated by the IGF binding proteins (IGFBPs).
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • IGF-I: Intra-assay: <10% Inter-assay: <15%
  • IGF-II: Intra-assay: <10% Inter-assay: <15%
Panel TypeMAGNETIC Endocrine
Quality LevelMQ200
Applications
ApplicationThe Human IGF Panel, using the Luminex xMAP technology, enables the simultaneous analysis of one or both IGF-I and IGF-II protein biomarkers in human serum, plasma and cell/tissue culture samples.
Key Applications
  • Multiplexing
Application Notes• This kit may be used for the analysis of Human IGF-I and/or II in Human serum, plasma, tissue culture supernatant or other biological samples.
• Sample extraction, using the reagents included in the kit, is required for serum and plasma samples. The final dilution for extracted serum or plasma samples is 1:60.
• Cell/tissue culture supernatants or extracts may require a dilution in appropriate control medium.
• The assay performs optimally with an overnight incubation (16-18 hours).
• Each well requires 25 uL of sample standard or control.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • IGF-I
  • IGF-II
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Gene Symbol
  • IGF1
  • Somatomedin-C
  • IGF-IB
  • IGF-IA
  • IBP1
  • MGF
  • IGFI
UniProt Number
Accuracy
  • IGF-I 83%
  • IGF-II 89%
Physicochemical Information
Sensitivity
  • MinDC+2SD
  • IGF-I 19 pg/mL
  • IGF-II 919 pg/mL
Linearity of DilutionContact technical support for this information
Standard Curve Range
  • IGF-I: approximately 41 - 30,000 pg/mL
  • IGF-II: approximately 892 - 650,000 pg/mL
  • Refer to analysis sheet for exact standard concentracions.
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Human IGF-I, II Magnetic Bead Panel

MILLIPLEX MAP Human IGF-I, II Magnetic Bead Panel - Endocrine Assay SDS

Title

Safety Data Sheet (SDS) 

References

Reference overviewPub Med ID
Effects of maternal obstructive sleep apnoea on fetal growth: a prospective cohort study.
Fung, AM; Wilson, DL; Lappas, M; Howard, M; Barnes, M; O'Donoghue, F; Tong, S; Esdale, H; Fleming, G; Walker, SP
PloS one  8  e68057  2013

Show Abstract
23894293 23894293

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
Protein Detection Platform and Services Brochure
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HIGFMAG-52K xPonent Protocol Template Version Magpix
HIGFMAG-52K. xPonent Standard Template Version Magpix
Quality Control Ranges: Milliplex HIGF-I, II Magnetic Bead Panel

Data Sheet

Title
Full Service Custom-built Assays (MilliporeSigma)

Posters

Title
MILLIPLEX MAP: Exercise, Signaling, and Your Brain (EMD)