HDIAB-34K-PMX5 | MILLIPLEX MAP Human Diabetes Panel, Premixed 5 Plex Assay

HDIAB-34K-PMX5
96-well plate  
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      C-Peptide, GLP-1 (Active), Glucagon, Insulin, Leptin H Mplex Luminex xMAP
      Description
      Catalogue NumberHDIAB-34K-PMX5
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX MAP Human Diabetes Panel, Premixed 5 Plex Assay
      OverviewEMD Millipore’s MILLIPLEX® MAP Human Diabetes Premixed Magnetic Bead Panel is a 5-plex kit to be used for the simultaneous quantification of the following analytes in serum, plasma or culture supernatant samples: C-Peptide, GLP-1 (Active), Glucagon, Insulin, Leptin.

      MILLIPLEX® MAP offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

      Each MILLIPLEX® MAP panel and kit includes:
      • Quality controls (QCs) provided to qualify assay performance

      • Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

      • Optimized serum matrix to mimic native analyte environment

      • Detection antibody cocktails designed to yield consistent analyte profiles within panel


      MILLIPLEX® MAP offers you:
      o The ability to choose any combination of analytes from our panel of seven analytes to design a custom kit that better meets your needs.
      o A convenient “all-in-one” box format that gives you the assurance that you will have all the necessary reagents you need to run your assay.

      EMD Millipore’s MILLIPLEX® MAP Human Diabetes Premixed Magnetic Bead Panel is part of the most versatile system available for metabolic hormone research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
      Background InformationType I Diabetes is an autoimmune disease (or a viral attack) that destroys pancreatic β-cells leaving the subject unable to produce insulin (usually early onset). Type II Diabetes is a metabolic disorder caused by obesity and a sedentary lifestyle (usually later onset). If the body is unable to produce sufficient levels of insulin, then Glucose levels will rise and in time, can cause heart disease, stroke, kidney failure and dementia, to name a few. The five analytes included in this kit are: insulin, glucagon, leptin, glucagon-like peptide (GLP-1 active), and C-Peptide (connecting peptide).

      The MILLIPLEX® MAP Human Diabetes Premixed Magnetic Bead Panel enables you to focus on the relevance and therapeutic potential of insulin, glucagon, leptin, GLP-1 (Active), and C-Peptide in your research model. Coupled with the Luminex xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of multiple analytes simultaneously, which can dramatically improve productivity.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • C-Peptide: Intra-assay <10% CV Inter-assay <15%
      • GLP-1 (Active): Intra-assay <10% CV Inter-assay <20% CV
      • Glucagon: Intra-assay <10% CV Inter-assay <15% CV
      • Insulin: Intra-assay <10% CV Inter-assay <15% CV
      • Leptin: Intra-assay <10% CV Inter-assay <15% CV
      Panel TypeMAGNETIC Metabolism
      Applications
      ApplicationThe Human Diabetes, Premixed 5 Plex Panel, using the Luminex xMAP technology, enables the simultaneous analysis of 5 protein biomarkers in human serum, plasma and cell culture samples.
      Key Applications
      • Multiplexing
      Application Notes• This kit is a 5-plex, premixed bead kit.
      • Two of the analytes require addition of DPPIV inhibitor (for GLP-1 active) and Aprotinin (for Glucagon) to the serum or plasma samples. Refer to the sample collection and storage section in the protocol for details.
      • The protocol recommends serum or plasma samples be run neat. Tissue/cell culture samples may require a dilution with an appropriate control medium prior to assay. • For optimal sensitivity use an overnight (16 - 18 hour) incubation.
      • Each well requires 25 uL of sample, standard or control.
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      Analytes Available
      • C-Peptide
      • GLP-1 (Active)
      • Glucagon
      • Insulin
      • Leptin
      Assay PanelEverything you need in a single kit.
      Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
      Accuracy
      • C-Peptide 92%
      • GLP-1 (Active) 86%
      • Glucagon 91%
      • Insulin 91%
      • Leptin 94%
      Physicochemical Information
      Sensitivity
      • MinDC+2SD
      • C-Peptide: 16.4 pg/mL
      • GLP-1 (Active): 2.1 pg/mL
      • Glucagon: 15.8 pg/mL
      • Insulin: 32.7 pg/mL
      • Leptin: 65.9 pg/mL
      Linearity of DilutionContact technical support for this information
      Standard Curve Range
      • C-Peptide: 27.4 - 20,000 pg/mL
      • GLP-1 (active): 2.7 - 2,000 pg/mL
      • Glucagon: Approximately 13.7 - 10,000 pg/mL (Refer to QC analysis sheet for exact concentration)
      • Insulin: 137 - 100,000 pg/mL
      • Leptin: 137 - 100,000 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Human Diabetes Magnetic Bead Panel

      SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
      Protein Detection Platform and Services Brochure

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      QUALITY CONTROL RANGES Milliplex Human Metabolic Hormone Magnetic Bead Panel

      Posters

      Title
      Multiplex Assays of Fifteen Human Complement Factors

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      Categories

      Life Science Research > Antibodies and Assays > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Premixed Kits
      Life Science Research > Protein Detection and Quantification > Immunoassays > Multiplexed Immunoassays > Milliplex MAP Assay Products > Premixed Kits