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HCYTMAG-60K-PX29 MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Premixed 29 Plex - Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Key Applications: Multiplexing Research Sub-Category: Inflammation & Autoimmune Mechanisms Detection Methods: Luminex xMAP
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HCYTMAG-60K-PX29
96-well plate  96 well plate
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsMultiplexing Ordering RequirementsDetection Methods
      EGF, G-CSF, GM-CSF, IFN-α2, IFN-γ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IP-10, MCP-1, MIP-1α, MIP-1β, TNF-α, TNF-β, VEGF, Eotaxin/CCL11 H Mplex Note: RANTES, PDGF-AA and PDGF-AB/AA cannot be plexed with other analytes for serum/plasma. Luminex xMAP
      Description
      Catalogue NumberHCYTMAG-60K-PX29
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Premixed 29 Plex - Immunology Multiplex Assay
      OverviewTo identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Magnetic Beads can make the process of automation and high throughput screening easier with features such as walk-away washing.

      The MILLIPLEX® MAP Human Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity..
      Background Information“Cytokine” is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. Also, unlike hormones, they are not produced by specialized cells which are organized in specialized glands. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Cytokine and chemokine research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, as well as disease states such as inflammatory disease, allergic reactions, irritable bowel disease (IBD), sepsis, and cancer.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Precision, %
      • Intra-assay: 4 - 13
      • Inter-assay: 5 - 17
      Multiplex Ordering RequirementsNote: RANTES, PDGF-AA and PDGF-AB/AA cannot be plexed with other analytes for serum/plasma.
      Panel TypeMAGNETIC Cytokines/Chemokines
      Quality LevelMQ200
      Applications
      ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
      Key Applications
      • Multiplexing
      Application Notes• This is an overnight or two-hour incubation assay; however, for best results an overnight incubation is recommended.

      • This assay requires 25 µl of neat plasma or serum, or 25 μL cell culture supernatant per well.

      • This kit may be used for the analysis of all or any combination of the cytokines and chemokines in serum, plasma or tissue/cell lysate and culture supernatant samples.
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • EGF
      • G-CSF
      • GM-CSF
      • IFN-α2
      • IFN-γ
      • IL-1α
      • IL-1β
      • IL-1ra
      • IL-2
      • IL-3
      • IL-4
      • IL-5
      • IL-6
      • IL-7
      • IL-8
      • IL-10
      • IL-12 (p40)
      • IL-12 (p70)
      • IL-13
      • IL-15
      • IL-17A
      • IP-10
      • MCP-1
      • MIP-1α
      • MIP-1β
      • TNF-α
      • TNF-β
      • VEGF
      • Eotaxin/CCL11
      Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Accuracy
      • 87% - 107%
      Physicochemical Information
      Sensitivity
      • Refer to kit protocol for sensitivities for individual cytokines/chemokines.
      Standard Curve Range
      • 3.2–10,000 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage for kit components is 2 - 8°C.
      Packaging Information
      Material Size96-well plate
      Material Package96 well plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Protocol: Human Cytokine / Chemokine Magnetic Bead Panel

      MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel - Premixed 29 Plex - Immunology Multiplex Assay SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
      Protein Detection Platform and Services Brochure
      The Power of Biomarker Analysis

      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      Quality Control Ranges: MILLIPLEX® MAP Human Cytokine/ Chemokine Magnetic Bead Panel
      Quality Control Ranges: Milliplex Human Cytokine / Chemokine Immunoassay

      Data Sheet

      Title
      Full Service Custom-built Assays (MilliporeSigma)

      Posters

      Title
      MILLIPLEX MAP: Exercise, Signaling, and Your Brain (EMD)