HCD8MAG-15K MILLIPLEX MAP Human CD8+ T Cell Magnetic Bead Panel - Immunology Multiplex Assay

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HCD8MAG-15K

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Overview

Replacement Information

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This panel is available for custom premix. Talk to your Protein Specialist today!

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
GM-CSF, sCD137, IFNγ, sFas, sFasL, Granzyme A, Granzyme B, IL-2, IL-4, IL-5, IL-6, IL-10, IL-13, MIP-1α, MIP-1β, TNF-α, Perforin H Mplex Luminex xMAP
Description
Catalogue NumberHCD8MAG-15K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human CD8+ T Cell Magnetic Bead Panel - Immunology Multiplex Assay
OverviewThe MILLIPLEX MAP Human CD8+ T Cell Magnetic Bead Panel enables you to focus on the therapeutic potential of Human CD8+ T Cell analytes. Coupled with the Luminex® xMAP® platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of several analytes simultaneously, which can dramatically improve productivity. The MILLIPLEX MAP Human CD8+ T Cell Magnetic Bead Panel is the most versatile system available for Mouse CD8+ T Cell research.

MILLIPLEX MAP offers you the ability to:

• Select a 17-plex premixed option or
• Choose any combination of analytes from our panel of 17 analytes to design a custom kit that better meets your needs.
• A convenient “all-in-one” box format gives you the assurance that you will have all the necessary reagents you need to run your assay.
Background InformationCD8+ T cells (also known as cytotoxic T cells, cytolytic T cells and killer T cells) belong to a sub-group of T cells capable of inducing the death of infected somatic or tumor cells. T cells that bind weakly to Major Histocompatibility Complex (MHC) self-antigens are positively selected into single-positive CD4+ or CD8+ T cells in the thymus. Those CD8+ T cells that survive and mature after activation become cytotoxic cells, expressing T-cell receptors (TCRs) capable of recognizing specific antigenic peptides bound to Class I MHC molecules and the glycoprotein CD8. Affinity between the CD8 protein and the MHC molecule helps to keep the cytotoxic T cell and target closely bound during antigen specific activation. Once activated, CD8+ T cells are generally classified as having a predefined cytotoxic role within the immune system and undergo IL-2 induced clonal expansion, which increases the number of cells specific for the target antigen. The CD8+ T cells then travel throughout the body in search of antigen-positive somatic/tumor cells.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 20
  • Intra-assay:< 10
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight incubation assay requiring 25 µL of neat plasma or serum, or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of all or any combination of the Human CD8+ T-Cell Panel analytes in serum, plasma or tissue/cell lysate and culture supernatant.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • GM-CSF
  • sCD137
  • IFNγ
  • sFas
  • sFasL
  • Granzyme A
  • Granzyme B
  • IL-2
  • IL-4
  • IL-5
  • IL-6
  • IL-10
  • IL-13
  • MIP-1α
  • MIP-1β
  • TNF-α
  • Perforin
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities of individual biomarkers.
Standard Curve Range
  • GM-CSF: 4 – 15,000 pg/mL
  • sCD137: 2 – 10,000 pg/mL
  • IFNγ:1 – 5,000 pg/mL
  • IL-10: 5 – 20,000 pg/mL
  • Granzyme A: 20 – 100,000 pg/mL
  • IL-13: 2 – 7,500 pg/mL
  • Granzyme B: 1 – 5,000 pg/mL
  • sFas: 400 – 1,650,000 pg/mL
  • IL-2: 2 -7,500 pg/mL
  • IL-4: 2 – 10,000 pg/mL
  • IL-5: 1 – 5,000 pg/mL
  • IL-6: 0.4 – 1,500 pg/mL
  • sFasL: 10 – 50,000 pg/mL
  • MIP-1α: 1 -3,500 pg/mL
  • MIP-1β: 7 – 30,000 pg/mL
  • TNFα: 0.5 – 2,000 pg/mL
  • Perforin: 10 – 50,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Transport Information
Supplemental Information
Specifications

Documentation

Protocols

Title
Protocol: Human CD8+ T-Cell Magnetic Bead Panel

MILLIPLEX MAP Human CD8+ T Cell Magnetic Bead Panel - Immunology Multiplex Assay SDS

Title

Safety Data Sheet (SDS) 

Brochure

Title
A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
Protein Detection Platform and Services Brochure
The Power of Biomarker Analysis

Technical Info

Title
96 well Template 2 Up
96 well Template Single
HCD8MAG-15K xPonent Protocol Template Version Magpix
HCD8MAG-15K xPonent Standard Template Version Magpix
Quality Control Ranges - Milliplex Human CD8+ T Cell Magnetic Bead Panel

Data Sheet

Title
Full Service Custom-built Assays (MilliporeSigma)

Posters

Title
MILLIPLEX MAP: Exercise, Signaling, and Your Brain (EMD)
MILLIPLEX MAP: The Immunology of Cancer (EMD)
MILLIPLEX® MAP Cell Signaling Wall Chart