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48-622MAG MILLIPLEX MAP 2-Plex Phospho/Total JNK Magnetic Bead Kit - Cell Signaling Multiplex Assay

48-622MAG
96-well plate  96-well plate
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      JNK (Total) JNK (Thr183/Tyr185) H, M, RMplexLuminex xMAP
      Description
      Catalogue Number48-622MAG
      Trade Name
      • MILLIPLEX
      DescriptionMILLIPLEX MAP 2-Plex Phospho/Total JNK Magnetic Bead Kit - Cell Signaling Multiplex Assay
      OverviewThe MILLIPLEX® MAP Total/Phospho Akt (Catalog #48-622MAG) has been developed for the simultaneous detection of phosphorylated JNK (Thr183/Tyr185) and total JNK in a single well using the Luminex system.
      References
      Product Information
      Detection methodLuminex xMAP
      ConfigurationPremixed
      Panel TypeCell Signaling
      Quality LevelMQ200
      Applications
      ApplicationIntracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples. The analytes available for this multiplex kit are: Erk (Total), Erk (Thr185/Tyr187).
      Key Applications
      • Multiplexing
      Application Noteso This is an overnight (4°C) incubation assay.
      o This kit must be run using the provided Assay Buffer.
      o This assay requires 25 μL diluted filtered cell lysate per well.
      o The suggested working range of protein concentration for the assay is 1 to 25 µg of total protein/well (25 µL/well at 40 to 1,000 µg/mL).
      Biological Information
      SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Analytes Available
      • JNK (Total)
      • JNK (Thr183/Tyr185)
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well plate
      Material Package96-well plate
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Protocol: 2-Plex Phospho/Total JNK Magnetic Bead Kit

      MILLIPLEX MAP 2-Plex Phospho/Total JNK Magnetic Bead Kit - Cell Signaling Multiplex Assay SDS

      Title

      Safety Data Sheet (SDS) 

      Brochure

      Title
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      Technical Info

      Title
      96 well Template 2 Up
      96 well Template Single
      Application Note: Bird¿s eye view of intracellular signal transduction pathways using customizable MILLIPLEX® MAP Cell Signaling Phospho/Total 2-Plex assays

      Data Sheet

      Title
      Full Service Custom-built Assays (MilliporeSigma)