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HMH3-34K MILLIPLEX HUMAN METABOLIC HORMONE PANEL V3

HMH3-34K
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Overview

Replacement Information
Replacement InformationHuman Metabolic Hormone Magnetic Bead Panel (HMHEMAG-34K).
Human Diabetes Panel, Premixed 5 Plex Assay (HDIAB-34K-PMX5).
Human Diabetes Panel- Premixed 5 Plex- Space Saver Bulk Packaging (HDIAB34KPMX5BK).

Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsMultiplexing Ordering RequirementsDetection Methods
Amylin (Total) C-Peptide Ghrelin (Active) GIP GLP-1 (Active) GLP-1 (Total) Glucagon IL-6 Insulin Leptin MCP-1 PP PYY TNFα Secretin Amylin (Active) HMplexPlease note: GLP-1 (Active) and GLP-1 (Total) cannot be plexed together. Amylin Active and Total cannot be plexed together.Luminex xMAP
Description
Catalogue NumberHMH3-34K
ReplacesHMHEMAG-34K, HDIAB-34K-PMX5, and HDIAB34KPMX5BK
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX HUMAN METABOLIC HORMONE PANEL V3
OverviewEMD Millipore’s MILLIPLEX® Human Metabolic Hormone Panel V3 is a 14-plex kit to be used for the simultaneous quantification of any or all of the following analytes in human serum, plasma, tissue/cell lysates, and culture supernatant samples: Amylin Active or Total, C-Peptide, Ghrelin Active, GIP, GLP-1 Active or Total, Glucagon, IL-6, Insulin, Leptin, MCP-1/CCL2, PP, PYY, Secretin and TNFα.

*Note: Amylin Active and Total cannot be run together in the same assay. GLP-1 Active an GLP-1 Total cannot be run together in the same assay.
Background InformationMetabolic syndrome is a cluster of conditions that occur together; it includes increased blood pressure, elevated insulin levels, excess body fat around the waist and abnormal cholesterol levels.

Key features of metabolic syndrome include insulin resistance, glucose intolerance, hypertension, dyslipidemia and central obsesity- all of which are risk factors for atherosclerosis, coronary heart disease, type 2 diabetes, kidney disease, and even premature death.

Adults with metabolic syndrome show a low-grade inflammation, whose link with obesity may be dysregulated adipocyte production of pro- and anti-inflammatory factors. Consequently, research done in this area covers multi-faceted fields of cytokines, acute phase proteins, diabetes, and obesity related hormones, as well as other cardiovascular disease biomarkers.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Intra-assay <10% CV
  • Inter-assay <20% CV
Multiplex Ordering RequirementsPlease note: GLP-1 (Active) and GLP-1 (Total) cannot be plexed together. Amylin Active and Total cannot be plexed together.
Panel TypeMAGNETIC Metabolism
Applications
ApplicationThe Human Metabolic Hormone Panel V3, using the Luminex xMAP technology, enables the simultaneous analysis of 14 protein biomarkers in human serum, plasma and tissue/lysate and culture supernatant samples.
Key Applications
  • Multiplexing
Application Notes•There are 16 biomarkers to choose from in this kit. However, this is a 14-plex kit since the following analytes cannot be run simultaneously: Amylin Active and Total cannot be run together in the same assay. GLP-1 Active and Total cannot be run together in the same assay.
• The following analytes require the addition of protease inhibitors to the serum or plasma samples (see sample collection and storage section in the protocol for details): GLP-1 (DPPIV inhibitor); Amylin (protease inhibitor cocktail); Glucagon (Aprotinin); Ghrelin (Active) (serine protease inhibitor).
• The protocol recommends serum or plasma samples be run neat. Tissue culture samples may be run neat or may require a dilution with an appropriate control medium.
• For optimal sensitivity, use an overnight (16 - 18 hour) incubation.
• Each well requires 25 uL of sample, standard or control
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Human
Analytes Available
  • Amylin (Total)
  • C-Peptide
  • Ghrelin (Active)
  • GIP
  • GLP-1 (Active)
  • GLP-1 (Total)
  • Glucagon
  • IL-6
  • Insulin
  • Leptin
  • MCP-1
  • PP
  • PYY
  • TNFα
  • Secretin
  • Amylin (Active)
Assay PanelEverything you need in a single kit.
Cross ReactivityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Accuracy
  • Amylin Total: 87%
  • C-Peptide: 89%
  • Ghrelin Active: 85%
  • GIP: 92%
  • GLP-1 Active: 92%
  • GLP-1 Total: 82%
  • Glucagon: 86%
  • IL-6: 93%
  • Insulin: 111%
  • Leptin: 87%
  • MCP-1: 92%
  • PP: 94%
  • PYY: 89%
  • TNFα: 86%
  • Secretin: 82%
  • Amylin Active: 91%
Physicochemical Information
Sensitivity
  • MinDC+2SD
  • Amylin Total: 11 pg/mL
  • C-Peptide: 17 pg/mL
  • Ghrelin Active: 15 pg/mL
  • GIP: 1 pg/mL
  • GLP-1 Active: 2.9 pg/mL
  • GLP-1 Total: 5 pg/mL
  • Glucagon: 10 pg/mL
  • IL-6: 1 pg/mL
  • Insulin: 57 pg/mL
  • Leptin: 52 pg/mL
  • MCP-1: 8 pg/mL
  • PP: 1 pg/mL
  • PYY: 21 pg/mL
  • TNFα: 1 pg/mL
  • Secretin: 12 pg/mL
  • Amylin Active: 9.2 pg/mL
Linearity of DilutionContact technical support for this information
Standard Curve Range
  • Amylin Total: 11 - 8,000 pg/mL
  • C-Peptide: 27 - 20,000 pg/mL
  • Ghrelin Active: 14 - 10,000 pg/mL
  • GIP: 1.9 - 1,400 pg/mL
  • GLP-1 Active: 3.4 - 2,500 pg/mL
  • GLP-1 Total: 3.4 - 2,500 pg/mL
  • Glucagon: 6.9 - 5,000 pg/mL
  • IL-6: 2.7 - 2,000 pg/mL
  • Insulin: 206 - 150,000 pg/mL
  • Leptin: 137 - 100,000 pg/mL
  • MCP-1: 6.9 - 5,000 pg/mL
  • PP: 2.7 - 2,000 pg/mL
  • PYY: 21 - 15,000 pg/mL
  • TNFα: 1.0 - 750 pg/mL
  • Secretin: 14 - 10,000 pg/mL
  • Amylin Active: 11 - 8,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications