BCYT1-33K MILLIPLEX® Bovine Cytokine/Chemokine Magnetic Bead Panel 1 - Immunology Multiplex Assay

BCYT1-33K

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Key Specifications Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
IFNγ, IL-1α, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-17A, MIP-1α, IL-36RA, IP-10, MCP-1, MIP-1β, TNFα, VEGF-A B Mplex Luminex xMAP
Description
Catalogue NumberBCYT1-33K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Bovine Cytokine/Chemokine Magnetic Bead Panel 1 - Immunology Multiplex Assay
OverviewThe MILLIPLEX® Bovine Cytokine/Chemokine Magnetic Bead Panel 1 is a 15-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, and tissue culture supernatant samples: IFNγ, IL-1α, IL-1β, IL-4, IL-6, IL-8 (CXCL8), IL-10, IL-17A, IL-36RA (IL-1F5), IP-10 (CXCL10), MCP-1 (CCL2), MIP-1α (CCL3), MIP-1β (CCL4), TNFα, VEGF-A.

MILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust. In addition to the assay characteristics listed in the protocol, other performance criteria evaluated during the validation process include: cross-reactivity, dilution linearity, kit stability, and sample behavior (e.g. detectability and stability). Each panel and kit meets stringent manufacturing criteria to ensure batch-to-batch reproducibility.

Each MILLIPLEX® panel and kit includes:
- Quality controls (QCs) provided to qualify assay performance

- Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency

- Optimized serum matrix to mimic native analyte environment

- Detection antibody cocktails designed to yield consistent analyte profiles within panel


The MILLIPLEX® Bovine Cytokine/Chemokine Magnetic Bead Panel 1 is part of the most versatile system available for cytokine and chemokine research. From our single to multiplex biomarker solutions, we partner with you to design, develop, analytically validate and build the most comprehensive library available for protein detection and quantitation.
Background InformationInflammation and immune cell response are key to maintaining homeostasis. Inflammation plays a central role in the etiology of many immunologically related diseases, such as infectious disease, osteoarthritis and respiratory disease, as well as neurologic, metabolic and cardiovascular disease and even cancer. However, until now the ability to measure levels of cytokines and chemokines in bovine samples has been limited primarily to ELISAs. Researchers can now measure multiple inflammatory biomarkers simultaneously using our MILLIPLEX® Bovine Cytokine/Chemokine Panel 1, based on the Luminex® xMAP® technology.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: <15
  • Intra-assay: <10
Panel TypeMAGNETIC Cytokines/Chemokines
Applications
ApplicationThe Bovine Cytokine/Chemokine bead-based multiplex cytokine panel, using the Luminex xMAP technology, enables the simultaneous analysis of 15 cytokine and chemokine biomarkers in bovine serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight (16-18 hours at 2-8 °C) or two-hour (20-25°C) incubation assay; although an overnight incubation is recommended.
• A maximum of 25 μL per well 1:2 diluted serum or plasma can be used. Tissue culture or other media may be run neat or appropriately diluted in control medium.
• This kit may be used for the analysis of all or any combination of the Bovine Cytokine/Chemokine Panel 1 analytes in serum, plasma or tissue/cell/tumor culture supernatant.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Bovine
Analytes Available
  • IFNγ
  • IL-1α
  • IL-1β
  • IL-4
  • IL-6
  • IL-8
  • IL-10
  • IL-17A
  • MIP-1α
  • IL-36RA
  • IP-10
  • MCP-1
  • MIP-1β
  • TNFα
  • VEGF-A
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • IFNγ: 94%
  • IL-1α: 94%
  • IL-1β: 91%
  • IL-4: 93%
  • IL-6: 95%
  • IL-8: 95%
  • IL-10: 96%
  • IL-17A: 103%
  • MIP-1α: 96%
  • IL-36RA: 98%
  • IP-10: 94%
  • MCP-1: 95%
  • MIP-1β: 97%
  • TNFα: 92%
  • VEGF-A: 97%
Physicochemical Information
Sensitivity
  • IFNγ: 0.08 pg/mL
  • IL-1α: 0.36 pg/mL
  • IL-1β: 4.93 pg/mL
  • IL-4: 16.57 pg/mL
  • IL-6: 11.23 pg/mL
  • IL-8: 22.6 pg/mL
  • IL-10: 1.05 pg/mL
  • IL-17A: 0.67 pg/mL
  • MIP-1α: 98.54 pg/mL
  • IL-36RA: 1.23 pg/mL
  • IP-10: 0.91 pg/mL
  • MCP-1: 2.74 pg/mL
  • MIP-1β: 2.32 pg/mL
  • TNFα: 22.62 pg/mL
  • VEGF-A: 1.18 pg/mL
Standard Curve Range
  • IFNγ: 0.1 – 2,000 pg/mL
  • IL-1α: 0.3 – 5,000 pg/mL
  • IL-1β: 2.6 – 40,000 pg/mL
  • IL-4: 12.8 – 200,000 pg/mL
  • IL-6: 2.6 – 40,000 pg/mL
  • IL-8: 2.2 – 35,000 pg/mL
  • IL-10: 0.96 – 15,000 pg/mL
  • IL-17A: 0.6 – 10,000 pg/mL
  • MIP-1α: 12.8 – 200,000 pg/mL
  • IL-36RA: 1.28 – 20,000 pg/mL
  • IP-10: 0.6 – 10,000 pg/mL
  • MCP-1: 1.92 – 30,000 pg/mL
  • MIP-1β: 2.2 – 35,000 pg/mL
  • TNFα: 12.8 – 200,000 pg/mL
  • VEGF-A: 1.28 – 20,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications