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13-104 MBP (myelin basic protein), bovine, purified

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13-104
10 mg  
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      Overview

      Replacement Information
      Description
      Catalogue Number13-104
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionMBP (myelin basic protein), bovine, purified
      OverviewBasally phosphorylated
      References
      Product Information
      HS Code3504 00 90
      Presentation10mM MOPS, pH 7.0, with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications
      • Enzyme Assays
      Application Notessuitable as substrate for numerous protein kinases such as MAP Kinase, cAMP-dependent Protein Kinase, Calmodulin-dependent Protein Kinase, Protein Kinase C, and Phosphorylase Kinase; MW: 18.4 kDa; frozen
      Biological Information
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
      Gene Symbol
      • MBP
      • MGC99675
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02686 # The classic group of MBP isoforms (isoform 4-isoform 14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non- classic group of MBP isoforms (isoform 1-isoform 3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T- cells and neural cells. Differential splicing events combined to optional post-translational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. Induces T-cell proliferation.
      SIZE: 304 amino acids; 33117 Da
      SUBUNIT: Homodimer; isoform 3 exists as a homodimer.
      SUBCELLULAR LOCATION: Myelin membrane; Peripheral membrane protein; Cytoplasmic side. Note=Cytoplasmic side of myelin.
      TISSUE SPECIFICITY: MBP isoforms are found in both the central and the peripheral nervous system, whereas Golli-MBP isoforms are expressed in fetal thymus, spleen and spinal cord, as well as in cell lines derived from the immune system.DEVELOPMENTAL STAGE: Expression turns on abruptly in fetus of 14 to 16 weeks. Even smaller isoforms seem to be produced during embryogenesis, some of these persisting in the adult. Expression of isoform MBP2 is more evident at 16 weeks and its relative proportion declined thereafter.
      PTM: Several charge isomers of MBP; C1 (the most cationic, least modified, and most abundant form), C2, C3, C4, C5, C6, C7, C8-A and C8-B (the least cationic form); are produced as a result of optional PTM, such as phosphorylation, deamidation of glutamine or asparagine, arginine citrullination and methylation. C8-A and C8-B contain each two mass isoforms termed C8-A(H), C8-A(L), C8-B(H) and C8-B(L), (H) standing for higher and (L) for lower molecular weight. C3, C4 and C5 are phosphorylated. The ratio of methylated arginine residues decreases in aging, making the protein more cationic. & The N-terminal alanine is acetylated (isoform 3, isoform 4, isoform 5 and isoform 6). & Arg-241 was found to be 6% monomethylated and 60% symmetrically dimethylated.
      DISEASE: SwissProt: P02686 # The reduction in the surface charge of citrullinated and/or methylated MBP could result in a weakened attachment to the myelin membrane. This mechanism could be operative in demyelinating diseases such as chronical multiple sclerosis (MS), and fulminating MS (Marburg disease).
      SIMILARITY: SwissProt: P02686 ## Belongs to the myelin basic protein family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by successfully phosphorylating MAP Kinase 2/Erk2, active (Catalog # 14-173)
      Sales RestrictionsThis product is derived from bovine source. Export of this product to certain countries may be restricted. Please contact Customer Service or your local distributor to inquire about product availability and export options.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions2 years at -20°C
      Packaging Information
      Material Size10 mg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      13-104 04053252729553

      Documentation

      MBP (myelin basic protein), bovine, purified SDS

      Title

      Safety Data Sheet (SDS) 

      MBP (myelin basic protein), bovine, purified Certificates of Analysis

      TitleLot Number
      MBP (myelin basic protein), bovine, purified - 16293 16293
      MBP (myelin basic protein), bovine, purified - 16401 16401
      MBP (myelin basic protein), bovine, purified - 17758 17758
      MBP (myelin basic protein), bovine, purified - 17804 17804
      MBP (myelin basic protein), bovine, purified - 18461 18461
      MBP (myelin basic protein), bovine, purified - 18674 18674
      MBP (myelin basic protein), bovine, purified - 19250 19250
      MBP (myelin basic protein), bovine, purified - 19522 19522
      MBP (myelin basic protein), bovine, purified - 20622 20622
      MBP (myelin basic protein), bovine, purified - 20761 20761

      References

      Reference overviewApplicationPub Med ID
      A novel non-canonical mechanism of regulation of MST3 (mammalian Sterile20-related kinase 3).
      Stephen J Fuller,Liam J McGuffin,Andrew K Marshall,Alejandro Giraldo,Sampsa Pikkarainen,Angela Clerk,Peter H Sugden
      The Biochemical journal  442  2012

      Show Abstract
      22229648 22229648
      Antidepressant-like effect of the novel thiadiazolidinone NP031115 in mice.
      Angelo O Rosa,Manuella P Kaster,Ricardo W Binfaré,Susana Morales,Ester Martín-Aparicio,Maria Luisa Navarro-Rico,Ana Martinez,Miguel Medina,Antonio G García,Manuela G López,Ana Lúcia S Rodrigues
      Progress in neuro-psychopharmacology & biological psychiatry  32  2008

      Show Abstract
      18579278 18579278
      Slit protein-mediated inhibition of CXCR4-induced chemotactic and chemoinvasive signaling pathways in breast cancer cells.
      Prasad, Anil, et al.
      J. Biol. Chem., 279: 9115-24 (2004)  2004

      Show Abstract
      Kinase Assay14645233 14645233
      Cep-1347 (KT7515), a semisynthetic inhibitor of the mixed lineage kinase family
      Maroney, A. C., et al
      J Biol Chem, 276:25302-8 (2001)  2001

      Kinase Assay11325962 11325962
      Ten ERK-related proteins in three distinct classes associate with AP-1 proteins and/or AP-1 DNA
      N V Kumar 1 , L R Bernstein
      J Biol Chem  276(34)  32362-72  2001

      Show Abstract
      Kinase Assay11431474 11431474
      Biomechanical stress-induced apoptosis in vein grafts involves p38 mitogen-activated protein kinases
      Mayr, M., et al
      Faseb J, 14:261-70 (2000)  2000

      Kinase Assay10660448 10660448
      A phosphatidylinositol 3-kinase-dependent pathway that differentially regulates c-Raf and A-Raf
      Sutor, S. L., et al
      J Biol Chem, 274:7002-10 (1999)  1999

      10066754 10066754
      Sequences within fibrinogen and intercellular adhesion molecule-1 (ICAM-1) modulate signals required for mitogenesis
      Gardiner, E. E. and D'Souza, S. E.
      J Biol Chem, 274:11930-6 (1999)  1999

      Kinase Assay10207014 10207014
      NSP1 defines a novel family of adaptor proteins linking integrin and tyrosine kinase receptors to the c-Jun N-terminal kinase/stress-activated protein kinase signaling pathway
      Lu, Y., et al
      J Biol Chem, 274:10047-52 (1999)  1999

      Kinase Assay10187783 10187783
      Beta-chemokine receptor CCR5 signals via the novel tyrosine kinase RAFTK
      Ganju, R. K., et al
      Blood, 91:791-7 (1998)  1998

      Kinase Assay9446638 9446638

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      Life Science Research > Proteins and Enzymes > Other Proteins