454854 MAP Kinase 2, His•Tag®, Human, Recombinant, E. coli

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454854
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454854-5UG
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      Description
      OverviewRecombinant, human MAP Kinase 2 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP Kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
      Catalogue Number454854
      Brand Family Calbiochem®
      SynonymsERK2, MAPK2, p42MAPK
      References
      ReferencesLew, J. 2003. Biochemistry 42, 849.
      Johnson, G.L., and Lapadat, R. 2002. Science 298, 1911.
      Koistinaho, M., and Koistinaho, J. 2002. Glia 40, 175.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
      FormLiquid
      FormulationIn 150 mM NaCl, 50 mM Tris, 1 mM DTT, 50% glycerol, pH 8.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Specific Activity≥5500 units/mg protein
      Physicochemical Information
      ContaminantsProteases: none detected
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      MAP Kinase 2, His•Tag®, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      MAP Kinase 2, His•Tag®, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      454854

      References

      Reference overview
      Lew, J. 2003. Biochemistry 42, 849.
      Johnson, G.L., and Lapadat, R. 2002. Science 298, 1911.
      Koistinaho, M., and Koistinaho, J. 2002. Glia 40, 175.

      Brochure

      Title
      MAPK Pathway Poster ( 750 KB )
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-August-2008 RFH
      SynonymsERK2, MAPK2, p42MAPK
      DescriptionRecombinant, human MAP Kinase 2 fused to a His•Tag® sequence and expressed in E. coli. Highly active form produced by phosphorylation of the purified MAP kinase in vitro with MEK1. Suitable for labeling MAP Kinase substrates.
      FormLiquid
      FormulationIn 150 mM NaCl, 50 mM Tris, 1 mM DTT, 50% glycerol, pH 8.5.
      Purity≥95% by SDS-PAGE
      ContaminantsProteases: none detected
      Specific activity≥5500 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol phosphate to myelin basic protein (MBP) per min at 30°C.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesLew, J. 2003. Biochemistry 42, 849.
      Johnson, G.L., and Lapadat, R. 2002. Science 298, 1911.
      Koistinaho, M., and Koistinaho, J. 2002. Glia 40, 175.