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71230 Lysonase™ Bioprocessing Reagent

71230
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71230-3
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      Glass bottle 0.2 ml
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      71230-4
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          Plastic ampoule 1 ml
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          Description
          Overview

          Lysonase™ Bioprocessing Reagent is an optimized, ready-to-use blend of rLysozyme™ Solution and Benzonase Nuclease. rLysozyme Solution contains a highly purified and stabilized recombinant lysozyme with specific activity 250 times greater than that of chicken egg white lysozyme. Benzonase Nuclease is a genetically engineered nonspecific endonuclease that degrades all forms of DNA and RNA (single stranded, double stranded, circular, linear), reducing extract viscosity and increasing protein yield. The combined activities of rLysozyme and Benzonase Nuclease significantly increase protein extraction efficiency and facilitate downstream processing of protein extracts. For efficient protein extraction with BugBuster® Protein Extraction Reagent, use 10 µl Lysonase per 1 g cell paste. For efficient protein extraction with PopCulture® Reagent, add 2 µl Lysonase per 1 ml culture. Store at –20°C.

          Catalogue Number71230
          Brand Family Novagen®
          References
          Product Information
          Quality LevelMQ400
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Lysonase™ Bioprocessing Reagent Certificates of Analysis

          TitleLot Number
          71230

          Brochure

          Title
          Benzonase®Nuclease
          Protein Purification and Detection Tools

          Citations

          Title
        • Johannes Mullegger, et al. (2005) Engineering of a thioglycoligase: randomized mutagenesis of the acid-base residue leads to the identification of improved catalysts. Protein Engineering Design and Selection 18, 33-40.
        • Dirk Hoffmeister and Jon S. Thorson. (2004) Mechanistic implications of Escherichia coli galactokinase structure-based engineering. ChemBioChem 5, 989-992.
        • Dirk Hoffmeister, et al. (2003) Creation of the first anomeric D/L-sugar kinase by means of directed evolution. Procedings of the National Academy of Science 100, 13184-13189.
        • User Protocols

          Title
          TB361 Lysonase™ Bioprocessing Reagent