71230 Lysonase™ Bioprocessing Reagent

71230
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71230-3
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      Glass bottle 0.2 ml
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      71230-4
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          Plastic ampoule 1 ml
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          71230-5
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              Plastic ampoule 5 ml
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              Description
              Overview

              Lysonase™ Bioprocessing Reagent is an optimized, ready-to-use blend of rLysozyme™ Solution and Benzonase Nuclease. rLysozyme Solution contains a highly purified and stabilized recombinant lysozyme with specific activity 250 times greater than that of chicken egg white lysozyme. Benzonase Nuclease is a genetically engineered nonspecific endonuclease that degrades all forms of DNA and RNA (single stranded, double stranded, circular, linear), reducing extract viscosity and increasing protein yield. The combined activities of rLysozyme and Benzonase Nuclease significantly increase protein extraction efficiency and facilitate downstream processing of protein extracts. For efficient protein extraction with BugBuster® Protein Extraction Reagent, use 10 µl Lysonase per 1 g cell paste. For efficient protein extraction with PopCulture® Reagent, add 2 µl Lysonase per 1 ml culture. Store at –20°C.

              Catalogue Number71230
              Brand Family Novagen®
              References
              Product Information
              Quality LevelMQ400
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Blue Ice Only
              Toxicity Standard Handling
              Storage -20°C
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              Lysonase™ Bioprocessing Reagent Certificates of Analysis

              TitleLot Number
              71230

              Brochure

              Title
              Protein Purification and Detection Tools

              Citations

              Title
            • Johannes Mullegger, et al. (2005) Engineering of a thioglycoligase: randomized mutagenesis of the acid-base residue leads to the identification of improved catalysts. Protein Engineering Design and Selection 18, 33-40.
            • Dirk Hoffmeister and Jon S. Thorson. (2004) Mechanistic implications of Escherichia coli galactokinase structure-based engineering. ChemBioChem 5, 989-992.
            • Dirk Hoffmeister, et al. (2003) Creation of the first anomeric D/L-sugar kinase by means of directed evolution. Procedings of the National Academy of Science 100, 13184-13189.
            • User Protocols

              Title
              TB361 Lysonase™ Bioprocessing Reagent