Key Specifications Table
|Key Applications||Detection Methods|
|TFX, IF, ICC, Track single cell and cell population migration (migration assay, cell culture)||Fluorescent|
|Description||LentiBrite™ RFP-Vimentin Lentiviral Biosensor|
|Overview||Read our application note in Nature Methods!
Learn more about the advantages of our LentiBrite Lentiviral Biosensors! Click Here
Biosensors can be used to detect the presence/absence of a particular protein as well as the subcellular location of that protein within the live state of a cell. Fluorescent tags are often desired as a means to visualize the protein of interest within a cell by either fluorescent microscopy or time-lapse video capture. Visualizing live cells without disruption allows researchers to observe cellular conditions in real time.
Lentiviral vector systems are a popular research tool used to introduce gene products into cells. Lentiviral transfection has advantages over non-viral methods such as chemical-based transfection including higher-efficiency transfection of dividing and non-dividing cells, long-term stable expression of the transgene, and low immunogenicity.
EMD Millipore is introducing LentiBrite™ Lentiviral Biosensors, a new suite of pre-packaged lentiviral particles encoding important and foundational proteins of autophagy, apoptosis, and cell structure for visualization under different cell/disease states in live cell and in vitro analysis.
EMD Millipore’s LentiBrite™ RFP-Vimentin lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of vimentin IF dynamics in difficult-to-transfect cell types.
|Background Information||Intermediate filaments (IFs) are 10 nm diameter cytoskeletal structures that typically form a cage-like structure extending from the nucleus to the plasma membrane. The five families of IF proteins have distinct polymer characteristics and cell type-specific expression patterns. Expression of vimentin, a type-III IF protein, is restricted to mesenchymal cells, and appearance of vimentin in epithelial cancer cells is a hallmark of the epithelial-to-mesenchymal transition. Vimentin assembles into classic long filaments and short filaments termed squiggles. Time-lapse microscopy of live cells expressing RFP-vimentin has revealed the dynamics of vimentin IF maturation, and crosstalk with microtubules and microfilaments.
EMD Millipore’s LentiBrite™ Vimentin-RFP lentiviral particles provide bright fluorescence and precise localization to enable live cell analysis of vimentin IF dynamics in difficult-to-transfect cell types.
|Purification Method||PEG precipitation|
|Safety Information according to GHS|
|Product Usage Statements|
|Quality Assurance||Evaluated by transduction of HT-1080 cells and fluorescent imaging performed for assessment of transduction efficiency.|
|Material Size||1 vial (minimum of 3 x 10E8 IFU/mL)|
LentiBrite™ RFP-Vimentin Lentiviral Biosensor SDS