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420411 Kanamycin Sulfate, Streptomyces kanamyceticus, Cell Culture-Tested - CAS 25389-94-0 - Calbiochem

Overview

Replacement Information

Key Specifications Table

CAS #Empirical Formula
25389-94-0C₁₈H₃₆N₄O₁₁ · H₂SO₄

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420411-5GM
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      Description
      OverviewAn aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 30S ribosome, causing misreading of the genetic code. May cause renal damage and ototoxicity.
      Contains >98% kanamycin A. Potency: ≥750 µg/mg
      Recommended working concentration: 100 µg/ml.
      Catalogue Number420411
      Brand Family Calbiochem®
      References
      ReferencesLech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1 (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.
      Cardon, G.H., et al. 1993. Plant J. 3, 773.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York. p.1.6, A.6.
      Korzybyski, T., et al. 1978. In Antibiotics, Origin, Nature, and Properties 1, 690.
      Stiffler, P.W., et al. 1974. Antimicrob. Agents Chemother. 6, 516.
      Umezawa, S., et al. 1968. J. Antibiot. 21, 367.
      Product Information
      CAS number25389-94-0
      FormWhite to off-white solid
      Hill FormulaC₁₈H₃₆N₄O₁₁ · H₂SO₄
      Chemical formulaC₁₈H₃₆N₄O₁₁ · H₂SO₄
      Quality LevelMQ100
      Applications
      Biological Information
      Primary Targetprotein biosynthesis that acts on the 30S ribosome
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSNZ3225030
      Safety Information
      R PhraseR: 36/37/38-42/43-60-61

      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      May impair fertility.
      May cause harm to the unborn child.
      S PhraseS: 26-36/37/39-45

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Carcinogenic / Teratogenic
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Kanamycin Sulfate, Streptomyces kanamyceticus, Cell Culture-Tested - CAS 25389-94-0 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Kanamycin Sulfate, Streptomyces kanamyceticus, Cell Culture-Tested - CAS 25389-94-0 - Calbiochem Certificates of Analysis

      TitleLot Number
      420411

      References

      Reference overview
      Lech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1 (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.
      Cardon, G.H., et al. 1993. Plant J. 3, 773.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York. p.1.6, A.6.
      Korzybyski, T., et al. 1978. In Antibiotics, Origin, Nature, and Properties 1, 690.
      Stiffler, P.W., et al. 1974. Antimicrob. Agents Chemother. 6, 516.
      Umezawa, S., et al. 1968. J. Antibiot. 21, 367.

      Brochure

      Title
      Antibiotics Profiler
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-September-2010 JSW
      DescriptionAn aminoglycoside antibiotic effective against Gram-positive and Gram-negative organisms. Inhibitor of protein biosynthesis that acts on the 70S ribosome, causing misreading of the genetic code. May cause renal damage and ototoxicity. Useful for the selection of kanamycin resistant plasmids in E. coli. Tested and found to be negative for cytotoxicity against HeLa cells. Contains more than 98% kanamycin A.
      FormWhite to off-white solid
      CAS number25389-94-0
      RTECSNZ3225030
      Chemical formulaC₁₈H₃₆N₄O₁₁ · H₂SO₄
      SolubilityH₂O (10 mg/ml)
      Storage +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, sterilize by filtration through a 0.22 µm pore-size filter, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 year at -20°C.
      Toxicity Carcinogenic / Teratogenic
      Merck USA index14, 5281
      ReferencesLech, K., and Brent, R. 1995. in Current Protocols in Mol. Biol. Vol. 1 (Ausubel, F.M., et al., Eds.) John Wiley & Sons, New York.
      Cardon, G.H., et al. 1993. Plant J. 3, 773.
      Maniatis, T., et al. 1989. In Molecular Cloning, A Laboratory Manual, Second Edition. Cold Spring Harbor, New York. p.1.6, A.6.
      Korzybyski, T., et al. 1978. In Antibiotics, Origin, Nature, and Properties 1, 690.
      Stiffler, P.W., et al. 1974. Antimicrob. Agents Chemother. 6, 516.
      Umezawa, S., et al. 1968. J. Antibiot. 21, 367.