532129 Intracellular pH Sensor, TPE-Cy - Calbiochem

532129
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₄₈H₄₃NO₃S

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      Glass bottle 5 mg
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      Description
      OverviewA cell-permeable, biocompatible tetraphenylethene-cyanine (TPE-Cy) adduct that acts as a two-channel ratiometric fluorogen and is sensitive to intracellular pH in the entire physiological range. Displays a large Stokes shift and exhibits aggregation-induced emission (AIE). Following its entry into live cells, it is distributed in the cytoplasm, accumulates in organelles, and becomes fluorescent. Interacts with the lipid components of the cells and shifts its color transition point of the red-to-blue emission from extracellular pH 10 to intracellular physiological pH range. At neutral pH it provides a strong blue emission (peak at 489 nm), which is enhanced at pH 8.07. However, acidification of medium causes a reduction in the blue emission (489 nm) and induces red emission (peak at 615 nm). The ratiometric red and blue signals can serve as an indicator for local proton concentration in the cell. Does not affect cell viability up to 12.5 µM concentration.

      Please note that the molecular weight for this compound is batch-specific due to variable water content.
      Catalogue Number532129
      Brand Family Calbiochem®
      SynonymspHi Sensor
      References
      ReferencesChen, S., et al. 2013. J. Am. Chem. Soc. 135, 4926.
      Product Information
      FormReddish orange powder
      Hill FormulaC₄₈H₄₃NO₃S
      Chemical formulaC₄₈H₄₃NO₃S
      Quality LevelMQ200
      Applications
      Biological Information
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Intracellular pH Sensor, TPE-Cy - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Chen, S., et al. 2013. J. Am. Chem. Soc. 135, 4926.

      Technical Info

      Title
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      White Paper: Further considerations of antibody validation and usage. (EMD)
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-March-2015 JSW
      SynonymspHi Sensor
      DescriptionA cell-permeable, biocompatible tetraphenylethene-cyanine (TPE-Cy) adduct that acts as a two-channel ratiometric fluorogen and is sensitive to intracellular pH in the entire physiological range. Displays a large Stokes shift and exhibits aggregation-induced emission (AIE). Following its entry into live cells, it is distributed in the cytoplasm, accumulates in organelles, and becomes fluorescent. Interacts with the lipid components of the cells and shifts its color transition point of the red-to-blue emission from extracellular pH 10 to intracellular physiological pH range. At neutral pH it provides a strong blue emission (peak at 489 nm), which is enhanced at pH 8.07. However, acidification of medium causes a reduction in the blue emission (489 nm) and induces red emission (peak at 615 nm). The ratiometric red and blue signals can serve as an indicator for local proton concentration in the cell. Does not affect cell viability up to 12.5 µM concentration.
      FormReddish orange powder
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₄₈H₄₃NO₃S
      Purity≥98% by HPLC
      SolubilityDMSO (100 mg/ml)
      Storage +2°C to +8°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesChen, S., et al. 2013. J. Am. Chem. Soc. 135, 4926.