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505224 InSolution™ Necrostatin-1 - Calbiochem

505224
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Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₁₃H₁₃N₃OS

Pricing & Availability

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      Glass bottle 10 mg
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      Description
      OverviewA cell-permeable, potent, and selective blocker of necroptosis (EC50 = 494 nM in FADD-deficient Jurkat cells treated with TNF-α), a nonapoptotic necrotic cell death pathway mediated by death-domain receptors (DRs) that offers neuroprotection in a murine model of ischemic brain injury. Exhibits no effect on DR-induced apoptosis. Also acts as a selective and ATP-competitive inhibitor of RIP1 kinase with negligible effect on RIP2 kinase activity. Nec-1 target appears to be a critical common necroptotic step upstream of execution events and downstream of DRs. Inactive control, Nec-1i, is also available (Cat. No. 480066).
      Catalogue Number505224
      Brand Family Calbiochem®
      SynonymsNecrosis Inhibitor II, 5-((1H-Indol-3-yl)methyl)-3-methyl-2-thioxoimidazolidin-4-one; 5-(Indol-3-ylmethyl)-(2-thio-3-methyl)hydantoin, MTH-Trp, Methyl-thiohydantoin-tryptophan, Nec-1, Nec1, Necrosome Inhibitor I, RIP1 Inhibitor I, RIPK 1 Inhibitor I, Receptor-Interacting Protein 1 Inhibitor I
      References
      ReferencesDegterev, A., et al. 2013. Nat. Chem. Biol. 9, 192.
      Degterev, A., et al. 2012. Cell Death Differ. 20, 366.
      Christofferson, D.E., et al. 2012. Cell Death Dis. 3, e320.
      Takahashi, N., et al. 2012. Cell Death Dis. 3, e437.
      Degterev, A., et al. 2008. Nat. Chem. Biol. 4, 313.
      Degterev, A., et al. 2005. Nat. Chem. Biol. 1, 112.
      Muller, A.J., et al. 2005. Nat. Med. 11, 312.
      Teng, X., et al. 2005. Bioorg. Med. Chem. Lett. 15, 5039.
      Product Information
      FormLiquid
      FormulationA 25 mM (10 mg/1.54 ml) sterile-filtered solution of Necrostatin-1 (Cat. No. 480065) in DMSO.
      Hill FormulaC₁₃H₁₃N₃OS
      Chemical formulaC₁₃H₁₃N₃OS
      Hygroscopic Hygroscopic
      ReversibleY
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥99% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/38

      Irritating to eyes and skin.
      S PhraseS: 26-36-45

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Irritant
      Storage ≤ -70°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      InSolution™ Necrostatin-1 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Degterev, A., et al. 2013. Nat. Chem. Biol. 9, 192.
      Degterev, A., et al. 2012. Cell Death Differ. 20, 366.
      Christofferson, D.E., et al. 2012. Cell Death Dis. 3, e320.
      Takahashi, N., et al. 2012. Cell Death Dis. 3, e437.
      Degterev, A., et al. 2008. Nat. Chem. Biol. 4, 313.
      Degterev, A., et al. 2005. Nat. Chem. Biol. 1, 112.
      Muller, A.J., et al. 2005. Nat. Med. 11, 312.
      Teng, X., et al. 2005. Bioorg. Med. Chem. Lett. 15, 5039.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-December-2013 JSW
      SynonymsNecrosis Inhibitor II, 5-((1H-Indol-3-yl)methyl)-3-methyl-2-thioxoimidazolidin-4-one; 5-(Indol-3-ylmethyl)-(2-thio-3-methyl)hydantoin, MTH-Trp, Methyl-thiohydantoin-tryptophan, Nec-1, Nec1, Necrosome Inhibitor I, RIP1 Inhibitor I, RIPK 1 Inhibitor I, Receptor-Interacting Protein 1 Inhibitor I
      DescriptionA cell-permeable, potent, and selective blocker of necroptosis (EC50 = 494 nM in FADD-deficient Jurkat cells treated with TNF-α), a nonapoptotic necrotic cell death pathway mediated by death-domain receptors (DRs) that offers neuroprotection in a murine model of ischemic brain injury. Exhibits no effect on DR-induced apoptosis. Also acts as a selective and ATP-competitive inhibitor of RIP1 kinase with negligible effect on RIP2 kinase activity. Nec-1 target appears to be a critical common necroptotic step upstream of execution events and downstream of DRs. Inactive control, Nec-1i, is also available (Cat. No. 480066).
      FormLiquid
      FormulationA 25 mM (10 mg/1.54 ml) sterile-filtered solution of Necrostatin-1 (Cat. No. 480065) in DMSO.
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₁₃H₁₃N₃OS
      Structure formulaStructure formula
      Purity≥99% by HPLC
      Storage ≤ -70°C
      Hygroscopic
      Protect from light
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.
      Toxicity Irritant
      ReferencesDegterev, A., et al. 2013. Nat. Chem. Biol. 9, 192.
      Degterev, A., et al. 2012. Cell Death Differ. 20, 366.
      Christofferson, D.E., et al. 2012. Cell Death Dis. 3, e320.
      Takahashi, N., et al. 2012. Cell Death Dis. 3, e437.
      Degterev, A., et al. 2008. Nat. Chem. Biol. 4, 313.
      Degterev, A., et al. 2005. Nat. Chem. Biol. 1, 112.
      Muller, A.J., et al. 2005. Nat. Med. 11, 312.
      Teng, X., et al. 2005. Bioorg. Med. Chem. Lett. 15, 5039.