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IPVH00010 Immobilon-P PVDF Membrane

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IPVH00010
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      Overview

      Replacement Information
      Replacement InformationINCP 000 10 (Protein Applications)

      Key Specifications Table

      Key Applications
      WB
      Description
      Catalogue NumberIPVH00010
      ReplacesINCP00010
      Trade Name
      • Immobilon
      DescriptionImmobilon-P PVDF Membrane
      Background InformationOverview
      - Millipore offers four different Immobilon PVDF membranes, each optimized for different protein blotting applications.
      Immobilon PVDF membranes provide many advantages compared to nitrocellulose. They won’t crack or curl, and they can be cut without fracturing. They also have low background, broad solvent compatibility, and superior staining capabilities. In addition, they can be reprobed multiple times.

      Immobilon-P Transfer Membrane:
      - 45 µm pore size Recommended for most western blotting, especially proteins greater than 20 kDa
      - Millipore's Rapid Immunodetection Protocol reduces detection times by up to 2 hours by eliminating membrane blocking and several wash steps with no loss of sensitivity or specificity

      Immobilon-E Membrane:
      - the only PVDF membrane that wets out in aqueous buffers, eliminating the methanol pre-wet step.
      - 45 µm pore size recommended for most western blotting, especially proteins greater than 20 kDa

      Blotting Sandwiches:
      - When you need to process multiple blots, blotting sandwiches are a convenient, time-saving choice. Our sandwiches include sheets of Immobilon-P membrane interleaved with pre-cut sheets of chromatography-grade blotting filter paper and are compatible with any standard wet transfer device.

      Blotting Filter Paper:
      - Chromatography-grade blotting filter paper pre-cut to the most popular western blotting sizes.

      Immobilon-PSQ Membrane:
      - 0.2 µm pore size and a large internal structure
      - Higher protein adsorption and sequencing yields than other membranes
      - Recommended for blotting proteins less than 20 kDa
      - Prevents blow-through of low molecular weight proteins

      Immobilon-FL Membrane:
      - The first transfer membrane optimized for fluorescence applications
      - Extremely low background improves sensitivity of all fluorescence detection protocols
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      - Ideal for multiplexing and chemifluorescence applications

      Immobilon® PVDF membranes are offered in four types, each optimized for a different protein blotting application. Convenient blotting sandwiches feature pre-cut sheets of membrane and blotting filter paper. Immobilon® PVDF membranes have high protein adsorption, so you won’t lose proteins during transfer or reprobing. The open pore structure makes it easy to access bound proteins and remove unbound probes. In addition, Immobilon® PVDF membranes optimized for fluorescent blots dramatically increase signal-to-noise ratios for high sensitivity in quantitative, multiplexing applications.

      Features & Benefits:
      - Won’t crack, curl or fracture when cut
      - Low background
      - Superior staining capabilities
      - Can be reprobed multiple times

      Applications:
      - Western Blotting, Immunoblotting, Dot Blotting, Protein Sequencing;
      - Compatible with Chemiluminescent, Fluorescent, Radioactive, Chromogenic, and Chemifluorescent Detection
      - Compatible with all commonly used fluorescent probes at all excitation and emission wavelengths
      References
      Product Information
      Detection methodChemiluminescence Chromogenic Radioactive
      Compatible Stains
      • Coomassie brilliant blue
      • Amido black
      • India ink
      • Ponceau-S red
      • Colloidal gold
      • CPTS
      • Toluidine blue
      • Transillumination
      • Sypro® ruby
      Filter CodeIPVH
      Filter ColorWhite
      Quality LevelMQ400
      Applications
      Application1 roll, 26.5 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.
      Key Applications
      • Western Blotting
      Biological Information
      Primary Binding MechanismsElectrostatic, hydrophobic
      BSA Adsorption215 µg/cm²
      Goat IgG Adsorption294 µg/cm²
      Insulin Adsorption160 µg/cm²
      WettabilityHydrophobic
      Physicochemical Information
      Pore Size0.45 µm
      Dimensions
      Filter SurfacePlain
      Filter Dimensions26.5 cm x 3.75 m
      Materials Information
      Chemistry
      • Hydrophobic Polyvinylidene Fluoride (PVDF)
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Packaging Information
      Material Size1
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Immobilon-P PVDF Membrane SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewApplicationPub Med ID
      Remodeling of VE-cadherin junctions by the human herpes virus 8 G-protein coupled receptor.
      Dwyer, J, et al.
      Oncogene, 30: 190-200 (2011)  2011

      Show Abstract
      20818438 20818438
      A CD36-dependent pathway enhances macrophage and adipose tissue inflammation and impairs insulin signalling.
      Kennedy, David J, et al.
      Cardiovasc. Res., 89: 604-13 (2011)  2011

      Show Abstract
      21088116 21088116
      Pulmonary artery smooth muscle cell senescence is a pathogenic mechanism for pulmonary hypertension in chronic lung disease.
      Noureddine, Hibo, et al.
      Circ. Res., 109: 543-53 (2011)  2011

      Show Abstract
      21719760 21719760
      Effects of HIV protease inhibitors on progression of monocrotaline- and hypoxia-induced pulmonary hypertension in rats.
      Gary-Bobo, Guillaume, et al.
      Circulation, 122: 1937-47 (2010)  2010

      Show Abstract
      20974998 20974998
      Novel irreversible epidermal growth factor receptor inhibitors by chemical modulation of the cysteine-trap portion.
      Carmi, Caterina, et al.
      J. Med. Chem., 53: 2038-50 (2010)  2010

      Show Abstract
      20151670 20151670
      Biochemical and functional properties of distinct nicotinic acetylcholine receptors in the superior cervical ganglion of mice with targeted deletions of nAChR subunit genes.
      David, Reinhard, et al.
      Eur. J. Neurosci., 31: 978-93 (2010)  2010

      Show Abstract
      20377613 20377613
      Parp1 facilitates alternative NHEJ, whereas Parp2 suppresses IgH/c-myc translocations during immunoglobulin class switch recombination.
      Robert, Isabelle, et al.
      J. Exp. Med., 206: 1047-56 (2009)  2009

      Show Abstract
      Immunoblotting (Western)19364882 19364882
      Interactions between Brucella suis VirB8 and its homolog TraJ from the plasmid pSB102 underline the dynamic nature of type IV secretion systems.
      Bourg, Gisèle, et al.
      J. Bacteriol., 191: 2985-92 (2009)  2009

      Show Abstract
      19251859 19251859

      Brochure

      Title
      SNAP i.d. 2.0 System Brochure
      Western Blotting Tools

      Technical Info

      Title
      Antibody recovery and reuse in the SNAP i.d.® 2.0 immunodetection system
      Protein Blotting Handbook: 6th Edition (MilliporeSigma)
      Protein Dot Blotting using Immobilon-P

      Data Sheet

      Title
      Immobilon Transfer Membranes: For superior protein and nucleic acid blots

      User Guides

      Title
      Immobilon -P Blotting Sandwiches
      Immobilon®-P Transfer Membrane User Guide
      Re-Blot Plus Western Blot Recycling Kit