400050 Hygromycin B, Streptomyces sp., Cell Culture-Tested - CAS 31282-04-9 - Calbiochem

Overview

Replacement Information

Key Specifications Table

Empirical FormulaCAS #
C₂₀H₃₇N₃O₁₃ 31282-04-9

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                  Description
                  OverviewUnique aminoglycoside antibiotic that inhibits the growth of prokaryotic (bacteria) and eukaryotic microorganisms (yeasts) and mammalian cells. Inhibits protein synthesis at the translocation step on the 70S ribosome and causes misreading of the mRNA. Hph, a gene from E. coli, encodes resistance to hygromycin B and can be isolated and cloned by recombinant DNA techniques. This hygromycin B-resistance gene is particularly useful for the identification or selection of recombinant clones in a variety of cell types. Hygromycin B penetrates cells that have been permeabilized by virus infection and can act as an effective antiviral agent. Working concentration: 50 µg/ml - 1 mg/ml for mammalian cell selection.
                  Potency: ≥1000 µ/mg solid.
                  Catalogue Number400050
                  Brand Family Calbiochem®
                  References
                  ReferencesDean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
                  Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
                  Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
                  Sugimoto, K., et al. 1994. Plant J. 5, 863.
                  MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
                  Bilang, R., et al. 1991. Gene 100, 247.
                  Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
                  Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
                  MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
                  Zhou, J., et al. 1991. Gene 107, 307.
                  Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
                  Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
                  Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
                  Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
                  Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
                  Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
                  Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
                  Kronstad, J.W., et al. 1989. Gene 79, 97.
                  Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
                  Cullen, D., et al. 1987. Gene 57, 21.
                  Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
                  Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
                  Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.
                  Product Information
                  CAS number31282-04-9
                  FormLight brown lyophilized solid
                  Hill FormulaC₂₀H₃₇N₃O₁₃
                  Chemical formulaC₂₀H₃₇N₃O₁₃
                  Structure formula ImageStructure formula Image
                  Quality LevelMQ100
                  Applications
                  Application CommentsProvided as a light brown lyophilized solid. Not provided under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 micron filter before use.
                  Biological Information
                  Biological activityThe activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.
                  Purity≥90% by HPLC and TLC
                  Physicochemical Information
                  Dimensions
                  Materials Information
                  Toxicological Information
                  Safety Information according to GHS
                  RTECSWK2130000
                  Safety Information
                  R PhraseR: 26/27/28-37/38-41

                  Very toxic by inhalation, in contact with skin and if swallowed.
                  Irritating to respiratory system and skin.
                  Risk of serious damage to eyes.
                  S PhraseS: 22-26-36/37/39-45

                  Do not breathe dust.
                  In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
                  Wear suitable protective clothing, gloves and eye/face protection.
                  In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
                  Product Usage Statements
                  Storage and Shipping Information
                  Ship Code Ambient Temperature Only
                  Toxicity Highly Toxic & Carcinogenic / Teratogenic
                  Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
                  Storage +2°C to +8°C
                  Do not freeze Ok to freeze
                  Special InstructionsAqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (< 2 mg/ml) are stable for up to 1 month at 4°C.

                  Note: This product is highly toxic. Read enclosed MSDS before handling.
                  Packaging Information
                  Transport Information
                  Supplemental Information
                  Specifications

                  Documentation

                  Hygromycin B, Streptomyces sp., Cell Culture-Tested - CAS 31282-04-9 - Calbiochem SDS

                  Title

                  Safety Data Sheet (SDS) 

                  Hygromycin B, Streptomyces sp., Cell Culture-Tested - CAS 31282-04-9 - Calbiochem Certificates of Analysis

                  TitleLot Number
                  400050

                  References

                  Reference overview
                  Dean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
                  Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
                  Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
                  Sugimoto, K., et al. 1994. Plant J. 5, 863.
                  MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
                  Bilang, R., et al. 1991. Gene 100, 247.
                  Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
                  Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
                  MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
                  Zhou, J., et al. 1991. Gene 107, 307.
                  Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
                  Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
                  Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
                  Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
                  Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
                  Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
                  Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
                  Kronstad, J.W., et al. 1989. Gene 79, 97.
                  Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
                  Cullen, D., et al. 1987. Gene 57, 21.
                  Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
                  Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
                  Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.

                  Brochure

                  Title
                  Antibiotics Profiler
                  Data Sheet

                  Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                  Revision26-September-2007 JSW
                  DescriptionHygromycin B, an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template (Dean, N., Gonzalez, A., et al., Rao, S.N., et al.). Hygromycin B has been used to select mutants in a wide variety of cells including bacteria (Bilang, R., et al., Salauze, D., et al.), protozoans (Lee, M.G-S., and Van der Ploeg, L.H.T.), yeast (Perlin, D.S., et al.), fungi (Cullen, D., et al., Kronstad, J.W., et al., Egelhoff, T.T., et al., Leslie, J.F., and Dickman, M.B., Bulte, L., and Bennoun, P.), plants (Dean, N., Damm, B., et al., Rikkerink, E. H., et al., Sugimoto, K., et al.), and mammalian cells (Crespi, C.L., et al., Giordano, T.J., and McAllister, W.T., Hubbard, S. C., et al.).

                  Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it (Bilang, R., et al., Malpartida, F., et al.). Hygromycin B is known to selectively penetrate cells that have been rendered permeable by virus infection. This, combined with its potency in inhibiting translation, makes it an effective antiviral agent (MacIntyre, G., et al., Zhou, J., et al.).

                  The analytical data listed below will vary from lot to lot.

                  Appearance: Off-white to light brown solid
                  Bioassay: 1000 U/mg
                  Solubility: ≥100 mg/ml in H2O
                  Purity (HPLC): ≥90%
                  Cytotoxicity: Tested in HeLa and/or CHO cells
                  BackgroundHygromycin B, an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template. Hygromycin B has been used to select mutants in a wide variety of cells including bacteria, protozoans, yeast, fungi, plants, and mammalian cells. Resistance to Hygromycin B is conferred by a gene coding for a phosphotransferase that phosphorylates Hygromycin B, thereby inactivating it. Hygromycin B is known to selectively penetrate cells that have been rendered permeable by virus infection. This combined with its potency in inhibiting translation, makes it an effective antiviral agent.
                  FormLight brown lyophilized solid
                  Recommended reaction conditions
                  Hygromycin B is not provided under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 µm filter before use. When using this product for mammalian cell selection the working concentration is generally between 50 µg/ml and 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type.
                  CAS number31282-04-9
                  RTECSWK2130000
                  Chemical formulaC₂₀H₃₇N₃O₁₃
                  Structure formulaStructure formula
                  Purity≥90% by HPLC and TLC
                  Biological activityThe activity of Hygromycin B is measured in a zonal inhibition assay using Bacillus subtilis. The activity is measured relative to a reference standard.
                  SolubilitydH₂O (≥100 mg/ml)
                  CommentsProvided as a light brown lyophilized solid. Not provided under sterile conditions. If a sterile solution is desired, working solutions of Hygromycin B in aqueous media can be passed through a 0.2 micron filter before use.
                  Storage +2°C to +8°C
                  Do Not Freeze Ok to freeze
                  Special InstructionsAqueous stock solutions should be kept at concentrations of ≥50 mg/ml and can be stored at either 4°C or -20°C. Aqueous stock solutions (≥50 mg/ml) are stable for up to 6 months at 4°C or -20°C. Working solutions (< 2 mg/ml) are stable for up to 1 month at 4°C.

                  Note: This product is highly toxic. Read enclosed MSDS before handling.
                  Toxicity Highly Toxic & Carcinogenic / Teratogenic
                  Merck USA index14, 4852
                  ReferencesDean, N. 1995. Proc. Natl. Acad. Sci. USA 92, 1287.
                  Hubbard, S. C., et al. 1994. J. Biol. Chem. 269, 3717.
                  Rikkerink, E. H., et al. 1994. Current Genetics 25, 202.
                  Sugimoto, K., et al. 1994. Plant J. 5, 863.
                  MacIntyre, G., et al. 1992. Adv. Exp. Med. Biol. 276, 67.
                  Bilang, R., et al. 1991. Gene 100, 247.
                  Lee, M.G-S., and Van der Ploeg, L. 1991. Gene 105, 255.
                  Leslie, J.F., and Dickman, M.B. 1991. Applied Environ. Microbiol. 57, 1423.
                  MacIntyre, G., et al. 1991. Antimicrob. Agents Chemother. 35, 2125.
                  Zhou, J., et al. 1991. Gene 107, 307.
                  Bulte, L., and Bennoun, P. 1990. Current Genetics 18, 155.
                  Giordano, T.J., and McAllister, W.T. 1990. Gene 88, 285.
                  Salauze, D., et al. 1990. Antimicrob. Agents Chemother. 24, 1915.
                  Carrasco, L., et al. 1989. Pharmacol. Ther. 9, 311.
                  Crespi, C.L., et al. 1989. Carcinogenesis 10, 295.
                  Damm, B., et al. 1989. Mol. Gen. Genetics 217, 6.
                  Egelhoff, T.T., et al. 1989. Mol. Cell. Biol. 9, 1965.
                  Kronstad, J.W., et al. 1989. Gene 79, 97.
                  Perlin, D.S., et al. 1988. J. Biol. Chem. 263, 118.
                  Cullen, D., et al. 1987. Gene 57, 21.
                  Gonzalez, A., et al. 1978. Biochim. Biophys. Acta 521, 459.
                  Malpartida, F., et al. 1983. Biochem. Biophys. Res. Commun. 117, 6.
                  Rao, S.N., et al. 1983. Antimicrob. Agents Chemother. 24, 689.