SCR502 Human, iPS Selection Kit

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SCR502
1 kit  Sufficient for 40 tests per antibody
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      ICC
      Description
      Catalogue NumberSCR502
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionHuman, iPS Selection Kit
      OverviewMillipore’s Human iPS Selection Kit is a quick, easy and non-invasive method to monitor the pluripotent state of live human ESC and fully reprogrammed human iPS cells using immunocytochemistry (ICC). The Human iPS Selection Kit allows live cell imaging and identification of fully reprogrammed human iPS cells from a heterogeneous population of reprogramming intermediates and enables the live selection of human iPS cells that can be further passaged and expanded for downstream applications. The selected live stained human iPS clones maintain normal human ESC morphology and can proliferate and be passaged normally. The kit consists of the following: (1) two conjugated antibodies that are known markers of human pluripotency, Tra-I-60 and SSEA-4, (2) a negative selection marker (CD13-PE) that is specific to fibroblasts and (3) a cell permeable Hoechst nuclear dye that possesses the dual function of labeling all cells and also distinguishing partially reprogrammed cells (Hoechst Bright) from fully reprogrammed cells (Hoechst Dim) (Chan et al,).
      Background InformationRecent findings indicate that fully reprogrammed human iPS cells possess the following characteristics: (1) they downregulate expression of fibroblast marker, CD13 (2) they upregulate expression of pluripotent markers, SSEA-4 and TRA-1-60, (3) they silence the viral transgenes while (4) reactivating endogenous expression of Nanog and (5) they assume a HoeschstDim phenotype. These characteristics have enabled reliable identification of fully reprogrammed human iPS cells from a mixed population of partially reprogrammed cells.
      References
      Product Information
      Components
      • Anti-SSEA-4 PE (Mouse monoclonal IgG3), Cat. No. CS204438. One vial containing 50 μg of 0.25 mg/mL purified mouse IgG antibody
      • Anti-TRA-1-60 FITC (Mouse monoclonal IgM), Cat. No. CS204460. One vial containing 50 μg of 0.5 mg/mL purified mouse IgM antibody
      • Anti-CD13 PE (Mouse monoclonal IgG1), Cat. No. CS204449. One vial containing 50 μg of 1 mg/mL purified mouse IgG antibody
      • Hoechst Nuclear Stain, Cat. No. CS204269. One vial containing 15 μL of 1 mg/mL stock solution.
      Quality LevelMQ100
      Applications
      ApplicationThe Human iPS Selection Kit allows live cell imaging & identification of fully reprogrammed human induced pluripotent Stem cells from a heterogeneous population of reprogramming intermediates.
      Key Applications
      • Immunocytochemistry
      Biological Information
      Species Reactivity
      • Human
      Stem Cell Type
      • Human Embryonic Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Maintain refrigerated at 2-8°C, protected from light in undiluted aliquots.
      Packaging Information
      Material Size1 kit
      Material PackageSufficient for 40 tests per antibody
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Human, iPS Selection Kit SDS

      Title

      Safety Data Sheet (SDS) 

      Human, iPS Selection Kit Certificates of Analysis

      TitleLot Number
      33804843380484
      Human iPS Selection Kit - 20631872063187
      Human iPS Selection Kit - 21095482109548
      Human iPS Selection Kit - 23157282315728
      Human iPS Selection Kit - 23630212363021
      Human iPS Selection Kit - 24693612469361
      Human iPS Selection Kit - 19906981990698
      Human iPS Selection Kit - 21427042142704
      Human iPS Selection Kit - 22688012268801
      Human iPS Selection Kit - 25301352530135

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      Categories

      Life Science Research > Cell Analysis > Cell-based Assays > Cell Characterization Assays