Millipore Sigma Vibrant Logo

HL-81K Human Leptin RIA

HL-81K
250 tubes  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Leptin H RIA Radioactive, 125I
      Description
      Catalogue NumberHL-81K
      DescriptionHuman Leptin RIA
      Background InformationLeptin is synthesized in adipocytes as a 16 kDa molecule and the circulating level is directly proportional to the total amount of fat in the body.
      References
      Product Information
      Detection methodRadioactive 125I
      Performance
      • ED80 = 0.9 ± 0.1 ng/ml
      • ED50 = 4.5 ± 0.4 ng/ml
      • ED20 = 22.2 ± 2.8 ng/ml
      Precision, %
      • Inter-assay: 3.6–6.2
      • Intra-assay: 3.4–8.3
      Quality LevelMQ100
      Applications
      ApplicationThis radioimmunoassay (RIA) measures & quantifies Leptin for use in Metabolism & Endocrine & Neuroscience research.
      Key Applications
      • Radioimmunoassay
      Application NotesOvernight, equilibrium incubation at 4°C. Sample volume 100 μL serum, plasma, or tissue culture media.
      Biological Information
      SpecificityHuman Leptin 100% Rat Leptin <0.2% Mouse Leptin <0.2% Human Insulin Not Detectable Human C-Peptide Not Detectable Glucagon, IGF-I Not Detectable Somatostatin, PP Not Detectable Rat Insulin Not Detectable Human Proinsulin Not Detectable Canine Lep
      Species Reactivity
      • Human
      Analytes Available
      • Leptin
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development.
      Gene Symbol
      • LEP
      • leptin
      • OB
      • obesity
      • OBS
      Radioactive Isotype125I-Human Leptin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P41159 # May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
      SIZE: 167 amino acids; 18641 Da
      SUBUNIT: Interacts with SIGLEC6.
      SUBCELLULAR LOCATION: Secreted.
      DISEASE:SwissProt: P41159 # Defects in LEP may be a cause of autosomal recessive obesity [MIM:601665].
      SIMILARITY: SwissProt: P41159 ## Belongs to the leptin family.
      Accuracy
      • 103–105%
      Physicochemical Information
      Linearity of Dilution88–101%
      Standard Curve Range
      • 0.5–100 ng/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size250 tubes
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Protocol: Human Leptin

      Human Leptin RIA SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Exercise training in obese older adults prevents increase in bone turnover and attenuates decrease in hip bone mineral density induced by weight loss despite decline in bone-active hormones.
      Krupa Shah,Reina Armamento-Villareal,Nehu Parimi,Suresh Chode,David R Sinacore,Tiffany N Hilton,Nicola Napoli,Clifford Qualls,Dennis T Villareal
      Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research  26  2011

      Show Abstract
      21786319 21786319
      Sex-specific effects of CNTF, IL6 and UCP2 polymorphisms on weight gain.
      A Geert Heidema,Ping Wang,Caroline T M van Rossum,Edith J M Feskens,Jolanda M A Boer,Freek G Bouwman,Pieter Van't Veer,Edwin C M Mariman
      Physiology & behavior  99  2010

      Show Abstract
      19833146 19833146
      Appetite responds to changes in meal content, whereas ghrelin, leptin, and insulin track changes in energy availability.
      Borer, Katarina T, et al.
      J. Clin. Endocrinol. Metab., 94: 2290-8 (2009)  2009

      Show Abstract
      19401375 19401375
      Effect of weight loss and exercise therapy on bone metabolism and mass in obese older adults: a one-year randomized controlled trial.
      Dennis T Villareal, Krupa Shah, Marian R Banks, David R Sinacore, Samuel Klein
      The Journal of clinical endocrinology and metabolism  93  2181-7  2008

      Show Abstract Full Text Article
      18364384 18364384
      Pioglitazone added to conventional lipid-lowering treatment in familial combined hyperlipidaemia improves parameters of metabolic control: relation to liver, muscle and regional body fat content.
      E Louise Thomas, Elizabeth Potter, Isabella Tosi, Julie Fitzpatrick, Gavin Hamilton, Vian Amber, Robert Hughes, Christopher North, Paul Holvoet, Mary Seed, D John Betteridge, Jimmy D Bell, Rossi P Naoumova
      Atherosclerosis  195  e181-90  2007

      Show Abstract
      17482623 17482623
      Energy expenditure, body composition and insulin response to glucose in male twins discordant for the Trp64Arg polymorphism of the beta3-adrenergic receptor gene.
      K Højlund, C Christiansen, K S Bjørnsbo, P Poulsen, L Bathum, J E Henriksen, O Lammert, H Beck-Nielsen
      Diabetes, obesity metabolism  8  322-30  2006

      Show Abstract
      16634992 16634992
      Bone mineral density response to caloric restriction-induced weight loss or exercise-induced weight loss: a randomized controlled trial.
      Dennis T Villareal, Luigi Fontana, Edward P Weiss, Susan B Racette, Karen Steger-May, Kenneth B Schechtman, Samuel Klein, John O Holloszy
      Archives of internal medicine  166  2502-10  2006

      Show Abstract
      17159017 17159017
      Effect of short-term fasting on urinary excretion of primary lipid peroxidation products and on markers of oxidative DNA damage in healthy women.
      Kyoung-Ho Lee, Helmut Bartsch, Jagadeesan Nair, Dong-Ho Yoo, Yun-Chul Hong, Soo-Hun Cho, Daehee Kang
      Carcinogenesis  27  1398-403  2006

      Show Abstract
      16401636 16401636
      Serum haptoglobin: a novel marker of adiposity in humans.
      C Chiellini, F Santini, A Marsili, P Berti, A Bertacca, C Pelosini, G Scartabelli, E Pardini, J López-Soriano, R Centoni, A M Ciccarone, L Benzi, P Vitti, S Del Prato, A Pinchera, M Maffei
      The Journal of clinical endocrinology and metabolism  89  2678-83  2004

      Show Abstract
      15181041 15181041
      Severe preeclampsia is associated with high inhibin A levels and normal leptin levels at 7 to 13 weeks into pregnancy.
      Laurent J Salomon, Clarisse Benattar, François Audibert, Hervé Fernandez, Michel Duyme, Joëlle Taieb, René Frydman
      American journal of obstetrics and gynecology  189  1517-22  2003

      Show Abstract
      14710054 14710054

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
      ELISAs, RIAs & GyroMark HT Assays
      Protein Detection Platform and Services Brochure

      Technical Info

      Title
      Quality Control Ranges: Human Leptin ELISA (Normal & Sensitive) Kit

      Data Sheet

      Title
      Full Service Custom-built Assays (MilliporeSigma)

      Related Products & Applications

      Related Products

      Catalog Number Description  
      HADK2MAG-61K MILLIPLEX MAP Human Adipokine Magnetic Bead Panel 2 - Endocrine Multiplex Assay Show Pricing & Availability
      HDIAB-34K-PMX5 MILLIPLEX MAP Human Diabetes Panel, Premixed 5 Plex Assay Show Pricing & Availability
      HMHEMAG-34K MILLIPLEX MAP Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay Show Pricing & Availability
      XL-85K Multi-Species Leptin RIA Show Pricing & Availability

      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Radioimmunoassay (RIA) > Complete RIA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Radioimmunoassay (RIA) > Complete RIA Kits