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CC1018 Human Integrin αVβ3 Protein, Triton X-100 Formulation

CC1018
25 µg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberCC1018
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionHuman Integrin αVβ3 Protein, Triton X-100 Formulation
      OverviewIntegrin alphaVbeta3 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to alphaVbeta3 integrin. A tissue detergent extract, which was applied to the column was prepared as previously described [Belkin, 1990].



      Two main protein bands corresponding to alphaV (145 kDa) and beta3 (92 kDa) subunits are seen in silver stained polyacrylamide gels under non-reducing conditions. Minor protein band (170 kDa) corresponds to beta3 subunit disulfide dimer.
      References
      Product Information
      PresentationPurified protein in 20 mM Tris-HCI, pH 7.5, 150 mM NaCI, 2 mM MgCI2, 0.2% Triton X-100. No preservatives.
      Quality LevelMQ100
      Applications
      Key Applications
      • Positive Control
      Application NotesElectrophoresis and Immunoblotting control. Also useful for in vitro binding

      experiments with metalloproteinase MMP-2 .
      Biological Information
      Concentration200 μg/mL
      Purity>95% by gel analysis

      7.5% SDS-PAGE by the method of Laemmli gives two major protein bands, corresponding to alpha V subunit (145 kDa) and beta 3 subunit (92 kDa) when visualized by silver stain (2-2.5 mg protein / lane nonreduced). A minor protein band (170 kDa) corresponds to the beta 3 dimer, similar to an occurrence of the beta 1 dimer upon purification of beta 1 integrins [Belkin et al., 1996]. In some cases a beta 3 trimer is visible at 260 kDa.

      After reduction, with protein quantities of 1.5 - 4μg protein / lane, three major protein bands are apparent, corresponding to beta 3 integrin subunit (110 kDa), heavy alpha V chain (125 kDa) and light alpha V chain (25 kDa). A minor band at 200 kDa region is presumed to be the reduced beta 3 dimer, consistent with previous publications describing a 230 kDa beta 1 reduced dimer for beta 1 integrin purifications described previously. [Belkin et al., 1996]. Faint bands at 80 kDa and 55 kDa correspond to minor degradation products of beta 3 .

      CONTAMINANTS: Two faint bands identified at 210 and 220 kDa, which may correspond to the alpha V dimer.
      Specific ActivityFunctional activity confirmed by interaction of integrin alphaV/beta3 with vitronectin, von Willebrand Factor, fibrinogen, and fibronectin in RGD-dependent ELISA testing.
      Entrez Gene Number
      Entrez Gene SummaryITAGV encodes integrin alpha chain V. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. The I-domain containing integrin alpha V undergoes post-translational cleavage to yield disulfide-linked heavy and light chains, that combine with multiple integrin beta chains to form different integrins. Among the known associating beta chains (beta chains 1,3,5,6, and 8; 'ITGB1', 'ITGB3', 'ITGB5', 'ITGB6', and 'ITGB8'), each can interact with extracellular matrix ligands; the alpha V beta 3 integrin, perhaps the most studied of these, is referred to as the Vitronectin receptor (VNR). In addition to adhesion, many integrins are known to facilitate signal transduction.
      Gene Symbol
      • ITGAV
      • DKFZp686A08142
      • MSK8
      • CD51
      • VNRA
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06756 # The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
      SIZE: 1048 amino acids; 116038 Da
      SUBUNIT: Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-V associates with either beta-1, beta-3, beta-5, beta-6 or beta-8 subunit. Interacts with HIV-1 Tat.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P06756 ## Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -70°C in undiluted aliquots. Avoid repeated freeze/ thaw cycles.
      Packaging Information
      Material Size25 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Human Integrin αVβ3 Protein, Triton X-100 Formulation SDS

      Title

      Safety Data Sheet (SDS) 

      Human Integrin αVβ3 Protein, Triton X-100 Formulation Certificates of Analysis

      TitleLot Number
      HUMAN INTEGRIN alphaVbeta3 TRITON X-100 FORMULATION PURIFIED PROTEIN - 2069337 2069337
      HUMAN INTEGRIN alphaVbeta3 TRITON X-100 FORMULATION PURIFIED PROTEIN - 2398862 2398862
      HUMAN INTEGRIN alphaVbeta3 TRITON X-100 FORMULATION - 3167112 3167112
      HUMAN INTEGRIN alphaVbeta3 TRITON X-100 FORMULATION - 3485028 3485028
      HUMAN INTEGRIN alphaVbeta3 TRITON X-100 FORMULATION - 3769805 3769805
      HUMAN INTEGRIN alphaVbeta3 TRITON X-100 FORMULATION -2665044 2665044
      HUMAN INTEGRIN alphaVbeta3 TRITON X-100 FORMULATION PURIFIED PROTEIN 2912690
      HUMAN INTEGRIN alphaVbeta3 TRITON X-100 FORMULATION PURIFIED PROTEIN - 2360929 2360929

      References

      Reference overviewPub Med ID
      Dentin matrix protein 1 enhances invasion potential of colon cancer cells by bridging matrix metalloproteinase-9 to integrins and CD44.
      Karadag, A; Fedarko, NS; Fisher, LW
      Cancer research  65  11545-52  2005

      Show Abstract Full Text Article
      16357164 16357164
      On the mechanism of thrombin-induced angiogenesis: involvement of alphavbeta3-integrin.
      Tsopanoglou, Nikos E, et al.
      Am. J. Physiol., Cell Physiol., 283: C1501-10 (2002)  2002

      Show Abstract
      12372811 12372811
      Beta 1 integrin subunit dimerization via disulfide bonds.
      Belkin, V M, et al.
      Biochem. Mol. Biol. Int., 40: 53-60 (1996)  1996

      Show Abstract
      8886269 8886269

      Data Sheet

      Title
      HUMAN INTEGRIN alphaVbeta3 TRITON X-100 FORMULATION PURIFIED PROTEIN