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GHRT-89HK Human Ghrelin (TOTAL) RIA

GHRT-89HK
125 tubes  
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Ghrelin H RIA Radioactive, 125I
      Description
      Catalogue NumberGHRT-89HK
      DescriptionHuman Ghrelin (TOTAL) RIA
      Background InformationGhrelin is a recently discovered hormone is secreted primarily by the stomach in response to glucose administration or CNS signals.
      References
      Product Information
      Detection methodRadioactive 125I
      Performance
      • ED80 = 395 ± 88 pg/mL
      • ED50 = 895 ± 177 pg/mL
      • ED20 = 2015 ± 382 pg/mL
      Precision, %
      • Inter-assay: 14.7–17.8
      • Intra-assay: 3.3–10.0
      Quality LevelMQ100
      Applications
      ApplicationThis radioimmunoassay (RIA) measures & quantifies Ghrelin for use in Metabolism & Endocrine & Neuroscience research.
      Key Applications
      • Radioimmunoassay
      Application NotesTwo-day disequilibrium assay at 4°C. Sample volume: < 100 μL Plasma (collected on ice) recommended
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • Ghrelin
      Entrez Gene Number
      Entrez Gene SummaryGhrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]
      Gene Symbol
      • GHRL
      • ghrelin
      • MTLRP
      • obestatin
      Radioactive Isotype125I-Human Ghrelin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UBU3 # Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
      SIZE: 117 amino acids; 12911 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
      PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28- C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28- C10:1, may be modified with a singly unsaturated carboxylic acid. & Amidation of Leu-98 is essential for obestatin activity (By similarity).
      SIMILARITY: SwissProt: Q9UBU3 ## Belongs to the motilin family.
      Accuracy
      • 90–96%
      Physicochemical Information
      Sensitivity
      • 100–10,000 pg/mL
      Linearity of Dilution99–146%
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size125 tubes
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Protocol: Ghrelin (Total) Radioimmunoassay (RIA) Kit

      Human Ghrelin (TOTAL) RIA SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Reverse Feeding Suppresses the Activity of the GH Axis in Rats and Induces a Preobesogenic State.
      Glad CA, Kitchen EE, Russ GC, Harris SM, Davies JS, Gevers EF, Gabrielsson BG, Wells T
      Endocrinology  152  869-82. Epub 2011 Jan 5.  2011

      Show Abstract
      21209022 21209022
      Cereal type and heat processing of the cereal affect nutrient digestibility and dynamics of serum insulin and ghrelin in weanling pigs.
      Menoyo D, Serrano MP, Barrios V, Valencia DG, Lázaro R, Argente J, Mateos GG
      Journal of animal science  2011

      Show Abstract
      21478449 21478449
      Acute differential effects of milk-derived dietary proteins on postprandial lipaemia in obese non-diabetic subjects.
      Holmer-Jensen J, Hartvigsen ML, Mortensen LS, Astrup A, de Vrese M, Holst JJ, Thomsen C, Hermansen K
      European journal of clinical nutrition  2011

      Show Abstract
      21792215 21792215
      Metabolic maturity at birth and neonate lamb survival: Association among maternal factors, litter size, lamb birth weight, and plasma metabolic and endocrine factors on survival and behavior.
      DR Miller, D Blache, RB Jackson, EF Downie, JR Roche
      Journal of animal science  88  581-93  2010

      Show Abstract
      19897628 19897628
      Effects of fat supplementation on postprandial GIP, GLP-1, ghrelin and IGFBP-1 levels: a pilot study on adolescents with type 1 diabetes.
      M Lodefalk,C Carlsson-Skwirut,J J Holst,J Aman,P Bang
      Hormone research in pædiatrics  73  2010

      Show Abstract
      20389106 20389106
      Basal Plasma Levels of Insulin, Leptin, Ghrelin, and Amylin Do Not Signal Adiposity in Rats Recovering from Forced Overweight.
      Gloy VL, Lutz TA, Langhans W, Geary N, Hillebrand JJ
      Endocrinology  2010

      Show Abstract
      20668029 20668029
      Comparison of Competitive Radioimmunoassays and Two-Site Sandwich Assays for the Measurement and Interpretation of Plasma Ghrelin Levels.
      Prudom C, Liu J, Patrie J, Gaylinn BD, Foster-Schubert KE, Cummings DE, Thorner MO, Geysen HM
      The Journal of clinical endocrinology and metabolism  2010

      Show Abstract
      20194708 20194708
      Ghrelin, paraoxonase and arylesterase levels in depressive patients before and after citalopram treatment.
      Abdullah Onder Barim, Suleyman Aydin, Ramis Colak, Ersel Dag, Omer Deniz, Ibrahim Sahin
      Clinical biochemistry  42  1076-81  2009

      Show Abstract
      19272368 19272368
      Metabolic maturity at birth and neonate lamb survival and growth: the effects of maternal low-dose dexamethasone treatment.
      D R Miller, R B Jackson, D Blache, J R Roche
      Journal of animal science  87  3167-78  2009

      Show Abstract
      19542513 19542513
      Appetite responds to changes in meal content, whereas ghrelin, leptin, and insulin track changes in energy availability.
      Borer, Katarina T, et al.
      J. Clin. Endocrinol. Metab., 94: 2290-8 (2009)  2009

      Show Abstract
      19401375 19401375

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
      ELISAs, RIAs & GyroMark HT Assays
      Protein Detection Platform and Services Brochure

      Technical Info

      Title
      Quality Control Ranges: Total Ghrelin RIA Kit

      Data Sheet

      Title
      Full Service Custom-built Assays (MilliporeSigma)

      FAQ

      QuestionAnswer
      What is the difference between active and total Ghrelin RIAs/ELISAs?The active assays require the intact octanoyl group on the 3rd AA which means samples need to be acidified and treated with aprotinin, while total/desacyl assays can read the truncated form without the octanoyl modification.

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Radioimmunoassay (RIA) > Complete RIA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Radioimmunoassay (RIA) > Complete RIA Kits