Millipore Sigma Vibrant Logo

GHRA-88HK Human Ghrelin (ACTIVE) RIA

GHRA-88HK
125 tubes  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Ghrelin H RIA Radioactive, 125I
      Description
      Catalogue NumberGHRA-88HK
      DescriptionHuman Ghrelin (ACTIVE) RIA
      Background InformationGhrelin is a recently discovered hormone is secreted primarily by the stomach in response to glucose administration or CNS signals.
      References
      Product Information
      Detection methodRadioactive 125I
      Performance
      • ED80 = 27 ± 7pg/mL
      • ED50=125 ± 33 pg/mL
      • ED20=652 ± 179 pg/mL
      Precision, %
      • Inter-assay: 9.6–16.2
      • Intra-assay: 6.5–9.5
      Quality LevelMQ100
      Applications
      ApplicationThis radioimmunoassay (RIA) measures & quantifies Ghrelin for use in Metabolism & Endocrine & Neuroscience research.
      Key Applications
      • Radioimmunoassay
      Application NotesTwo-day disequilibrium assay at 4°C. Sample volume: < 100 μL Plasma (collected on ice) recommended
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • Ghrelin
      Entrez Gene Number
      Entrez Gene SummaryGhrelin is an endogenous ligand for the growth hormone secretagogue receptor (GHSR; MIM 601898) and is involved in regulating growth hormone (GH; MIM 139250) release. Ghrelin is derived from a preprohormone called preproghrelin, which also generates a second peptide called obestatin. Obestatin is an endogenous ligand for the orphan G protein-coupled receptor GPR39 (MIM 602886) and is involved in satiety and decreased food intake.[supplied by OMIM]
      Gene Symbol
      • GHRL
      • ghrelin
      • MTLRP
      • obestatin
      Radioactive Isotype125I-Human Ghrelin
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UBU3 # Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility (By similarity).
      SIZE: 117 amino acids; 12911 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Highest level in stomach. All forms are found in serum as well. Other tissues compensate for the loss of ghrelin synthesis in the stomach following gastrectomy.
      PTM: O-octanoylation or O-decanoylation is essential for ghrelin activity. The O-decanoylated forms Ghrelin-27-C10 and Ghrelin-28- C10 differ in the length of the carbon backbone of the carboxylic acid bound to Ser-26. A small fraction of ghrelin, ghrelin-28- C10:1, may be modified with a singly unsaturated carboxylic acid. & Amidation of Leu-98 is essential for obestatin activity (By similarity).
      SIMILARITY: SwissProt: Q9UBU3 ## Belongs to the motilin family.
      Accuracy
      • 83–114%
      Physicochemical Information
      Sensitivity
      • 10 pg/mL
      Linearity of Dilution100–142.5%
      Standard Curve Range
      • 10–2000 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size125 tubes
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Protocol: Ghrelin (Active) Radioimmunoassay (RIA) Kit

      Human Ghrelin (ACTIVE) RIA SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Total and acylated ghrelin levels in children with poor growth.
      Jordan E Pinsker,Deborah Ondrasik,Debora Chan,Gregory J Fredericks,Eludrizza Tabisola-Nuesca,Minela Fernandez-Aponte,Dean R Focht,Merrily Poth
      Pediatric research  69  2011

      Show Abstract
      21386753 21386753
      Acute differential effects of milk-derived dietary proteins on postprandial lipaemia in obese non-diabetic subjects.
      Holmer-Jensen J, Hartvigsen ML, Mortensen LS, Astrup A, de Vrese M, Holst JJ, Thomsen C, Hermansen K
      European journal of clinical nutrition  2011

      Show Abstract
      21792215 21792215
      Metabolic state alters economic decision making under risk in humans.
      Symmonds, Mkael, et al.
      PLoS ONE, 5: e11090 (2010)  2010

      Show Abstract
      20585383 20585383
      Comparison of Competitive Radioimmunoassays and Two-Site Sandwich Assays for the Measurement and Interpretation of Plasma Ghrelin Levels.
      Prudom C, Liu J, Patrie J, Gaylinn BD, Foster-Schubert KE, Cummings DE, Thorner MO, Geysen HM
      The Journal of clinical endocrinology and metabolism  2010

      Show Abstract
      20194708 20194708
      Effect of feed restriction and supplemental dietary fat on gut peptide and hypothalamic neuropeptide messenger ribonucleic acid concentrations in growing wethers.
      Relling AE, Pate JL, Reynolds CK, Loerch SC
      Journal of animal science  88  737-48  2009

      Show Abstract
      19897637 19897637
      Ghrelin, paraoxonase and arylesterase levels in depressive patients before and after citalopram treatment.
      Abdullah Onder Barim, Suleyman Aydin, Ramis Colak, Ersel Dag, Omer Deniz, Ibrahim Sahin
      Clinical biochemistry  42  1076-81  2009

      Show Abstract
      19272368 19272368
      Restricting time at pasture: effects on dairy cow herbage intake, foraging behavior, hunger-related hormones, and metabolite concentration during the first grazing session.
      Gregorini P, Clark CE, Jago JG, Glassey CB, McLeod KL, Romera AJ
      Journal of dairy science  92  4572-4580  2009

      Show Abstract
      19700720 19700720
      Mastication of almonds: effects of lipid bioaccessibility, appetite, and hormone response.
      Bridget A Cassady, James H Hollis, Angie D Fulford, Robert V Considine, Richard D Mattes
      The American journal of clinical nutrition  89  794-800  2009

      Show Abstract
      19144727 19144727
      Long-term infusions of ghrelin and obestatin in early lactation dairy cows.
      J R Roche, A J Sheahan, L M Chagas, D Blache, D P Berry, J K Kay
      Journal of dairy science  91  4728-40  2008

      Show Abstract
      19038949 19038949
      Short communication: change in plasma ghrelin in dairy cows following an intravenous glucose challenge.
      J R Roche, A J Sheahan, L M Chagas, R C Boston
      Journal of dairy science  91  1005-10  2008

      Show Abstract
      18292256 18292256

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
      ELISAs, RIAs & GyroMark HT Assays
      Protein Detection Platform and Services Brochure

      Technical Info

      Title
      Quality Control Ranges: Active Ghrelin RIA Kit

      Data Sheet

      Title
      Full Service Custom-built Assays (MilliporeSigma)

      FAQ

      QuestionAnswer
      What is the difference between active and total Ghrelin RIAs/ELISAs?The active assays require the intact octanoyl group on the 3rd AA which means samples need to be acidified and treated with aprotinin, while total/desacyl assays can read the truncated form without the octanoyl modification.

      Related Products & Applications

      Related Products

      Catalog Number Description  
      EZGRA-88BK Human Ghrelin (active) ELISA- Bulk Show Pricing & Availability
      EZGRA-88K Human Ghrelin (active) ELISA Show Pricing & Availability
      GHRT-89HK Human Ghrelin (TOTAL) RIA Show Pricing & Availability
      HMHEMAG-34K MILLIPLEX MAP Human Metabolic Hormone Magnetic Bead Panel - Metabolism Multiplex Assay Show Pricing & Availability

      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Radioimmunoassay (RIA) > Complete RIA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Radioimmunoassay (RIA) > Complete RIA Kits