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EZHADP-61K Human Adiponectin ELISA

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      Replacement Information
      Replacement InformationThis product replaces CYT350, CHEMICON® Human Adiponectin ELISA Kit

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Adiponectin HELISAFluorescent Colorimetric
      Catalogue NumberEZHADP-61K
      DescriptionHuman Adiponectin ELISA
      Background InformationAdiponectin is identified as a 30 kDa adipokine that is secreted from the adipose tissue into circulating blood and influences systemic metabolism.
      Product Information
      Detection methodFluorescent Colorimetric
      Precision, %
      • Inter-assay: 1.0–7.4 , 2.4–8.4
      • Intra-assay: 1.0–7.4
      Quality LevelMQ100
      ApplicationThis Human Adiponectin ELISA is used to measure & quantify Adiponectin levels in Metabolism & Endocrine research.
      Key Applications
      • ELISA
      Application NotesRoom Temperature, 3 hour assay using 20 µL sample size (serum/plasma: 1:500 dilution)
      Biological Information
      Species Reactivity
      • Human
      Analytes Available
      • Adiponectin
      Entrez Gene Number
      Entrez Gene SummaryThis gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes.
      Gene Symbol
      • ADIPOQ
      • adiponectin
      • AdipoQ
      • apM-1
      • APM1
      • ACDC
      • GBP28
      • APM-1
      • ACRP30
      • apM1
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q15848 # Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
      SIZE: 244 amino acids; 26414 Da
      SUBUNIT: Homomultimer. Forms trimers, hexamers and 12- to 18-mers. The trimers (low molecular weight complexes / LMW) are assembled via non-covalent interactions of the collagen-like domains in a triple helix and hydrophobic interactions within the globular C1q domain. Several trimers can associate to form disulfide-linked hexamers (middle molecular weight complexes / MMW) and larger complexes (higher molecular weight / HMW). The HMW-complex assembly may rely aditionnally on lysine hydroxylation and glycosylation. LMW, MMW and HMW complexes bind to HBEGF, MMW and HMW complexes bind to PDGFB, and HMW complex binds to FGF2.
      TISSUE SPECIFICITY: Synthesized exclusively by adipocytes and secreted into plasma.DOMAIN:SwissProt: Q15848 The C1q domain is commonly called the globular domain.
      PTM: Hydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. & HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin- sensitizing activity of adiponectin in hepatocytes (By similarity). & O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. & Not N-glycosylated.
      DISEASE: SwissProt: Q15848 # Defects in ADIPOQ are the cause of adiponectin deficiency [MIM:605441]. The result is a very low concentration of plasma adiponectin. Decreased adiponectin plasma levels are associated with obesity insulin resistance, and diabetes type 2.
      SIMILARITY: Contains 1 C1q domain. & Contains 1 collagen-like domain.
      MISCELLANEOUS: Variants Arg-84 and Ser-90 show impaired formation of HMW complexes whereas variants Cys-112 and Thr-164 show impaired secretion of adiponectin in any form. & HMW-complex blood contents are higher in females than in males, are increased in males by castration and decreased again upon subsequent testosterone treatment, which blocks HMW- complex secretion (By similarity). In type 2 diabetic patients, both the ratios of HMW to total adiponectin and the degree of adiponectin glycosylation are significantly decreased as compared with healthy controls.
      • 92–102%
      Physicochemical Information
      • 1.5 ng/mL
      Linearity of Dilution81–100%
      Standard Curve Range
      • 1.5–100 ng/mL adiponection
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • For research use only. Not for use in diagnostic procedures.
      Storage and Shipping Information
      Packaging Information
      Material Size96-well strip plate
      Transport Information
      Supplemental Information



      Protocol: Human Adiponectin ELISA Kit

      Human Adiponectin ELISA SDS


      Safety Data Sheet (SDS) 


      Reference overviewPub Med ID
      Effects of acute hyperinsulinaemia on total and high-molecular-weight adiponectin concentration in metabolically healthy but obese postmenopausal women: a Montreal-Ottawa New Emerging Team (MONET) study.
      Elisha B, Karelis AD, Imbeault P, Rabasa-Lhoret R
      Diabetes Metab  36  319-21. Epub 2010 Jun 4.  2010

      Show Abstract
      20605505 20605505
      Adiponectin changes in HCV-Genotype 4: relation to liver histology and response to treatment.
      M Derbala, N Rizk, S Al-Kaabi, A Amer, F Shebl, A Al Marri, I Aigha, D Alyaesi, H Mohamed, H Aman, N Basem
      Journal of viral hepatitis  16  689-96  2009

      Show Abstract
      19486470 19486470
      Adiponectin levels and its association with hyperglycaemia in adult Filipino participants in the 2003--04 National Nutrition and Health Survey.
      Paz-Pacheco E, Lim-Abrahan MA, Sy RA, Jasul GV, Sison CM, Laurel AF
      Diab Vasc Dis Res  6  231-7  2009

      Show Abstract
      20368216 20368216
      Effect of antipsychotics on peptides involved in energy balance in drug-naive psychotic patients after 1 year of treatment.
      Rocio Perez-Iglesias,Jose Luis Vazquez-Barquero,Jose Antonio Amado,Ana Berja,Maria Teresa Garcia-Unzueta,Jose María Pelayo-Terán,Eugenio Carrasco-Marín,Ignacio Mata,Benedicto Crespo-Facorro
      Journal of clinical psychopharmacology  28  2008

      Show Abstract
      18480685 18480685
      Analytical validation and biological evaluation of a high molecular-weight adiponectin ELISA.
      Madhur K Sinha, Traci Songer, Qiang Xiao, John H Sloan, Jin Wang, Shaoquen Ji, William E Alborn, Randy A Davis, Michael M Swarbrick, Kimber L Stanhope, Bruce M Wolfe, Peter J Havel, Todd Schraw, Robert J Konrad, Philipp E Scherer, Jehangir S Mistry
      Clinical chemistry  53  2144-51  2007

      Show Abstract
      17951289 17951289


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      Technical Info

      96 well Template 2 Up
      96 well Template Single
      Quality Control Range Sheet- HAE-118 and HAE-218
      Quality Control Ranges: HUMAN ADIPONECTIN ELISA -Lot 115_215
      Quality Control Ranges: Human Adiponectin ELISA Lot 117-217

      Data Sheet

      Full Service Custom-built Assays

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      Life Science Research > Antibodies and Assays > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits
      Life Science Research > Protein Detection and Quantification > Immunoassays > Enzyme-linked Immunosorbent Assay (ELISA) > Complete ELISA Kits