375228 Hepatocyte Growth Factor, Human, Recombinant, S. frugiperda

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375228
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Overview

Replacement Information

Key Specifications Table

Purity
≥95% by SDS-PAGE

Pricing & Availability

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375228-5UG
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      Plastic ampoule 5 μg
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      Description
      OverviewRecombinant, human hepatocyte growth factor expressed in S. frugiperda insect cells. Pleiotropic growth factor that has also been called scatter factor, hepatopoietin A, and mammary growth factor. Through binding to its high affinity met receptor, HGF has marked and varied effects on hepatocytes and other epithelial and endothelial cell types. HGF-induced proliferation of primary hepatocytes is mediated by activation of PI 3-kinase. Acts as a potent mitogen for mature hepatocytes.
      Catalogue Number375228
      Brand Family Calbiochem®
      SynonymsrhHGF
      References
      ReferencesKaido, T., et al. 1996. Biochem. Biophys. Res. Commun. 218, 1.
      Kobayashi, Y., et al. 1996. Biochem. Biophys. Res. Commun. 220, 7.
      Skouteris, G.G., et al. 1996. Biochem. Biophys. Res. Commun. 218, 229.
      Rubin, J.S., et al. 1993. Biochim. Biophys. Acta 1155, 357.
      Strain, A.J. 1993. J. Endocrinol. 137, 1. Nakamura, T., et al. 1989. Nature 342, 440.
      Product Information
      FormLyophilized
      FormulationLyophilized from a sterile-filtered solution in 20 mM Sodium Phosphate, 0.5 M NaCl, 50 µg BSA/µg HGF, pH 7.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityED₅₀ = 20-40 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation in an HGF-responsive epithelial cell line, 4MBr-5
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      Contaminantsendotoxin: ≤1 EnU/µg HGF
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 1 month at 4°C or for up to 3 months at -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Hepatocyte Growth Factor, Human, Recombinant, S. frugiperda SDS

      Title

      Safety Data Sheet (SDS) 

      Hepatocyte Growth Factor, Human, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      375228

      References

      Reference overview
      Kaido, T., et al. 1996. Biochem. Biophys. Res. Commun. 218, 1.
      Kobayashi, Y., et al. 1996. Biochem. Biophys. Res. Commun. 220, 7.
      Skouteris, G.G., et al. 1996. Biochem. Biophys. Res. Commun. 218, 229.
      Rubin, J.S., et al. 1993. Biochim. Biophys. Acta 1155, 357.
      Strain, A.J. 1993. J. Endocrinol. 137, 1. Nakamura, T., et al. 1989. Nature 342, 440.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision28-November-2018 JSW
      SynonymsrhHGF
      DescriptionRecombinant, human hepatocyte growth factor expressed in Spodoptera frugiperda insect cells. Pleiotropic growth factor that has also been called scatter factor, hepatopoietin A, and mammary growth factor. Through binding to its high affinity met receptor, HGF has marked and varied effects on hepatocytes and other epithelial and endothelial cell types. HGF-induced proliferation of primary hepatocytes is mediated by activation of PI 3-kinase. Acts as a potent mitogen for mature hepatocytes.
      FormLyophilized
      FormulationLyophilized from a sterile-filtered solution in 20 mM Sodium Phosphate, 0.5 M NaCl, 50 µg BSA/µg HGF, pH 7.0.
      Purity≥95% by SDS-PAGE
      Contaminantsendotoxin: ≤1 EnU/µg HGF
      Biological activityED₅₀ = 20-40 ng/ml as measured by its ability to stimulate ³H-thymidine incorporation in an HGF-responsive epithelial cell line, 4MBr-5
      SolubilityReconstitute to a concentration of ≥1 µg/ml in sterile PBS containing ≥0.1% HSA or BSA.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for 1 month at 4°C or for up to 3 months at -70°C.
      Toxicity Standard Handling
      ReferencesKaido, T., et al. 1996. Biochem. Biophys. Res. Commun. 218, 1.
      Kobayashi, Y., et al. 1996. Biochem. Biophys. Res. Commun. 220, 7.
      Skouteris, G.G., et al. 1996. Biochem. Biophys. Res. Commun. 218, 229.
      Rubin, J.S., et al. 1993. Biochim. Biophys. Acta 1155, 357.
      Strain, A.J. 1993. J. Endocrinol. 137, 1. Nakamura, T., et al. 1989. Nature 342, 440.