Hemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.
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Description
Overview
Stable solid containing more than 60% protein. Protein is 90% undissociated hemocyanin.
Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3
Catalogue Number
374805
Brand Family
Calbiochem®
Synonyms
KLH
References
References
Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37. Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137. Erlanger, B.F. 1980. Methods Enzymol.70, 85.
Product Information
CAS number
9013-72-3
Form
Blue to blue-gray lyophilized solid
Formulation
Lyophilized from BES buffer, MgSO₄.
Preservative
None
Applications
Application
Hemocyanin, Keyhole Limpet, Megathura crenulata, CAS 9013-72-3, is a large, multi-subunit, oxygen-carrying, metalloprotein that is used as a carrier protein in the production of antibodies.
Application Comments
Protein concentration: ≥60% Copper: ≥0.09% Copper to Protein ratio: ≥1.5 x 10-3
Biological Information
Purity
≥90% by size exclusion chromatography
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
+2°C to +8°C
Do not freeze
Ok to freeze
Special Instructions
Dissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).
Hemocyanin, Keyhole Limpet, Megathura crenulata Certificates of Analysis
Title
Lot Number
374805
References
Reference overview
Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37. Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137. Erlanger, B.F. 1980. Methods Enzymol.70, 85.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
10-March-2011 JSW
Synonyms
KLH
Description
Hemocyanin purified from Keyhole Limpet (Mega thura crenulata). Protein:≥60%; copper to protein ratio: ≥1.5 x 10-3
Background
Keyhole limpet hemocyanin (KLH), a carrier protein with a molecular weight ranging from 3,000,000-7,500,000, has many available primary amines to facilitate protein conjugation. KLH is used to immunize animals to elicit antibodies to small molecules (haptens) by covalent conjugation.
Form
Blue to blue-gray lyophilized solid
Formulation
Lyophilized from BES buffer, MgSO₄.
CAS number
9013-72-3
Purity
≥90% by size exclusion chromatography
Preservative
None
Comments
Protein concentration: ≥60% Copper: ≥0.09% Copper to Protein ratio: ≥1.5 x 10-3
Storage
+2°C to +8°C
Do Not Freeze
Ok to freeze
Special Instructions
Dissolve at 5-10 mg/ml (protein) in PBS or other physiological buffer by GENTLE addition of the buffer (e.g. slowly added down the side of the tube). DO NOT vortex or agitate vigorously to dissolve product. (KLH is an extremely large protein complex and has a tendency to aggregate and precipitate from solutions.) For further dilutions, use an appropriate buffer and maintain the magnesium concentration at ≥10 mM. Solutions should be stored in the refrigerator (4°C).
Toxicity
Standard Handling
Merck USA index
14, 4647
References
Hayashi, K., et al. 1992. Virchows Archiv. B Cell Pathol. 63, 37. Shuler, K.R., et al. 1992. J. Immunol. Methods 156, 137. Erlanger, B.F. 1980. Methods Enzymol.70, 85.
