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03-227 HDAC3/NCOR1 complex human recombinant

03-227
50 µg  
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      Overview

      Replacement Information

      Key Specifications Table

      Key ApplicationsSpecies
      Enzyme AssaysHuman
      Description
      Catalogue Number03-227
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionHDAC3/NCOR1 complex human recombinant
      OverviewHuman recombinant HDAC3/NCOR1 produced in insect cells
      Background InformationUnlike other class I HDAC enzymes, HDAC3 is found in co-repressor complexes with the SMRT/N-CoR proteins. HDAC3 contains a unique domain structure which includes both nuclear localization and nuclear export sequences. HDAC3 has been implicated in cancer, as HDAC3 inhibitors produce anti-proliferative effects in various cancer cells. It may also play a role in heart disease, which may involve deacetylation of MEF2.
      References
      Product Information
      FormatPurified
      PresentationIn liquid containing 50 mM Tris, pH 8.0, 138 mM NaCl and 10% glycerol. Frozen solution.
      Quality LevelMQ100
      Applications
      ApplicationHuman recombinant HDAC3/NCOR1 produced in insect cells.
      Key Applications
      • Enzyme Assays
      Application NotesHDAC deacetylase assays
      Biological Information
      PurityPurity >90% by SDS-PAGE
      SourceHuman HDAC3 and the activation domain of human NCOR1
      Specific Activity>150 U/µg (based on total protein;>250 U/µg based on weight of HDAC3). 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity
      Molecular Weight49/40 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC3/NCOR1 into separate tubes before storing at -80°C.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

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      Categories

      Life Science Research > Proteins and Enzymes > Other Enzymes