CBA086 Glucose Assay Kit

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CBA086
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Overview

Replacement Information

Key Specifications Table

Detection Methods
Colorimetric or Fluorimetric

Pricing & Availability

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CBA086-1KIT
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      Description
      OverviewThe Glucose Assay kit provides direct measurement of glucose in various biological samples (e.g. serum, plasma, body fluids). Glucose enzyme mix oxidizes glucose to generate a product which reacts with a dye to produce color and fluorescence. The generated color or fluorescence is proportional to the amount of glucose in the sample.
      Catalogue NumberCBA086
      Brand Family Calbiochem®
      Application Data
      References
      ReferencesLee, Y.S. et al. 2007.Diabetes 56, 1671.
      Product Information
      Detection methodColorimetric or Fluorimetric
      Form100 tests
      Format96-well plate
      Kit containsGlucose Assay Buffer, Glucose Probe, Glucose Enzyme Mix, and Glucose Standard, and a user protocol.
      Quality LevelMQ100
      Applications
      Biological Information
      Assay range1-200 µM
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 21/22-36/38

      Harmful in contact with skin and if swallowed.
      Irritating to eyes and skin.
      S PhraseS: 24/25-26-36-45

      Avoid contact with skin and eyes.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      Product Usage Statements
      Intended useThe Glucose Assay Kit provides direct measurement of glucose in various biological samples (e.g., serum, plasma, body fluid, food, growth medium, etc.). The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose levels during fermentation and glucose feeding in protein expression processes.
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Storage ConditionsUpon arrival store the entire contents of the kit at (-20°C). Protect from light. Note: Allow reagents warm to room temperature and briefly centrifuge vials before opening.
      Do not freeze Ok to freeze
      Mercury prohibited To comply with ban of sale of mercury-added products required by The Interstate Mercury Education and Reduction Clearinghouse (IMERC), this product is prohibited to be sold in the following US states: Rhode Island and Connecticut.
      Packaging Information
      Transport Information
      Supplemental Information
      Kit containsGlucose Assay Buffer, Glucose Probe, Glucose Enzyme Mix, and Glucose Standard, and a user protocol.
      Specifications

      Documentation

      Glucose Assay Kit SDS

      Title

      Safety Data Sheet (SDS) 

      Glucose Assay Kit Certificates of Analysis

      TitleLot Number
      CBA086

      References

      Reference overview
      Lee, Y.S. et al. 2007.Diabetes 56, 1671.
      User Protocol

      Revision17-September-2012 JSW
      Form100 tests
      Format96-well plate
      Detection methodColorimetric or Fluorimetric
      StorageUpon arrival store the entire contents of the kit at (-20°C). Protect from light. Note: Allow reagents warm to room temperature and briefly centrifuge vials before opening.
      Intended useThe Glucose Assay Kit provides direct measurement of glucose in various biological samples (e.g., serum, plasma, body fluid, food, growth medium, etc.). The method is rapid, simple, sensitive, and suitable for high throughput. The assay is also suitable for monitoring glucose levels during fermentation and glucose feeding in protein expression processes.
      BackgroundGlucose (C6H12O6; FW: 180.16) is a very important fuel source for generating universal energy molecules ATP. Glucose level is a key diagnostic parameter for many metabolic disorders. Measurement of glucose can be very important in both research and drug discovery processes.
      Principles of the assayThe Glucose Assay Kit utilizes the Glucose Enzyme Mix to specifically oxidize glucose to generate a product that reacts with a dye to generate color (&lambda = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The generated color and fluorescence is proportional to the glucose amount.
      Materials provided• Glucose Assay Buffer (Kit Component No. JA9439-25ML): 1 bottle, 25 ml
      • Glucose Probe (in DMSO, anhydrous) (Kit Component No. JA9440-1EA): 1 vial
      • Glucose Enzyme Mix (Kit Component No. JA9442-1EA): 1 vial
      • Glucose Standard (100 nmol/µl) (Kit Component No. JA9443-0.1ML): 1 vial, 100 µl
      PreparationPrepare test samples in a total volume of 50 µl/well with Glucose Assay Buffer. Serum can be directly diluted in Glucose Assay Buffer; typically 0.5-2 µl/assay is sufficient (normal serum contains ~5 nmol glucose/µl). We suggest testing several doses of your sample to make sure the readings are within the standard curve range.
      Reagent preparation• Glucose Enzyme Mix: Dissolve the Glucose Enzyme Mix in 220 µl Glucose Assay Buffer. Aliquot and store at -20°C. Use within two months.
      • Glucose Probe: Supplied ready to use. Warm to room temperature to thaw the DMSO.
      • Standard Curve: For colorimetric assay, dilute the Glucose Standard to 1 nmol/ml by adding 10 µl of the Glucose Standard to 990 µl of Glucose Assay Buffer, mix well. Add 0, 2, 4, 6, 8, 10 µl into each well individually. Adjust volume to 50 µl/well with Glucose Assay Buffer to generate 0, 2, 4, 6, 8, 10 nmol/well of Glucose Standard. For fluorometric assay, dilute the Glucose Standard solution to 0.1 nmol/µl by adding 10 µl of the Glucose Standard to 990 µl of Glucose Assay Buffer, mix well. Then take 20 µl into 180 µl of Glucose Assay Buffer. Mix well. Add 0, 2, 4, 6, 8, 10 µl into each well individually. Adjust volume to 50 µl/well with Glucose Assay Buffer to generate 0, 0.2, 0.4, 0.6, 0.8, 1.0 nmol/well of the Glucose Standard.
      Detailed protocol1. Prepare reagent mix as indicated below; prepare a volume that is sufficient for the number of assays to be performed; 50 µl per well is required. Each 50 µl of reaction mix should contain the following components:
      • 46 µl Glucose Assay Buffer
      • 2 µl Glucose Probe
      • 2 µl Glucose Enzyme Mix
      Mix well.
      2. Add 50 µl Reaction Mix to each well containing a diluted Glucose Standard or test sample(s). Mix well.
      3. Incubate the reaction for 30 min at 37°C, protect from light.
      4. Measure the absorbance at 570 nm for a colorimetric assay or at excitation/emission = 535/590 nm for a fluorometric assay using an appropriate microplate reader
      5. Correct for background by subtracting the value derived from the 'zero' glucose control from all Standard and sample readings. Note: The background reading can be significant and MUST be subtracted from Standard and sample readings. Glucose concentrations of the test samples can then be calculated based on the standard curve generated.
      Standard curve

      Figure 1: Standard Curve

      Assay Range1-200 µM
      Registered TrademarksCalbiochem® is a registered trademark of , KGaA, Darmstadt.
      InteractivePathways™ is a trademark of , KGaA, Darmstadt.