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GL-32K Glucagon RIA

GL-32K
250 tubes  
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      Overview

      Replacement Information

      Key Specifications Table

      Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
      Glucagon H, M, R RIA Radioactive, 125I
      Description
      Catalogue NumberGL-32K
      DescriptionGlucagon RIA
      Background InformationGlucagon is a 3,5 kDa hormone secreted by the A cells of the Islets of Langerhans in the pancreas and is involved in regulation of glucose homeostasis thro µgh enhanced synthesis and mobilization of glucose in the liver, and regulation of glucose-stimulated insulin secretion.
      References
      Product Information
      Detection methodRadioactive 125I
      Performance
      • ED80 = 44 ± 4 pg/mL
      • ED50 = 109 ± 5 pg/mL
      • ED20 = 264 ± 17 pg/mL
      Precision, %
      • Inter-assay: 11.7 ± 3.0
      • Intra-assay: 4.9 ± 1.3
      Quality LevelMQ100
      Applications
      ApplicationThis radioimmunoassay (RIA) measures & quantifies Glucagon for use in Metabolism & Endocrine research.
      Key Applications
      • Radioimmunoassay
      Application NotesTwo-day disequilibrium incubation at 4°C. Sample volume < 100 μL serum, plasma, or tissue culture media.
      Biological Information
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Analytes Available
      • Glucagon
      Cross ReactivityGlucagon is conserved in almost all mammals. This kit may be used to measure glucagon in these samples (e.g. rat, mouse, etc.)
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is actually a preproprotein that is cleaved into four distinct mature peptides. One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis. Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation. Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms. Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
      Gene Symbol
      • GCG
      • GLP1
      • glucagon
      • GRPP
      • GLP2
      Radioactive Isotype125I-Glucagon
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01275 # Glicentin may modulate gastric acid secretion and the gastro-pyloro-duodenal activity. May play an important role in intestinal mucosal growth in the early period of life.
      SIZE: 180 amino acids; 20909 Da
      SUBCELLULAR LOCATION: Secreted.
      TISSUE SPECIFICITY: Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP1 and GLP2 are also secreted in selected neurons in the brain.
      PTM: Proglucagon is post-translationally processed in a tissue- specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.
      SIMILARITY: SwissProt: P01275 ## Belongs to the glucagon family.
      MISCELLANEOUS: In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo.
      Accuracy
      • 97 ± 0.8%
      Physicochemical Information
      Standard Curve Range
      • 20–400 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size250 tubes
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Protocols

      Title
      Glucagon Radioimmunoassay (RIA) Kit

      Glucagon RIA SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overviewPub Med ID
      Inhibition of Prohormone Convertases PC1/3 and PC2 by 2,5-Dideoxystreptamine Derivatives.
      Mirella Vivoli,Thomas R Caulfield,Karina Martínez-Mayorga,Alan T Johnson,Guan-Sheng Jiao,Iris Lindberg
      Molecular pharmacology  81  2012

      Show Abstract
      22169851 22169851
      Beneficial effects of exendin-4 on experimental polyneuropathy in diabetic mice.
      Himeno, T; Kamiya, H; Naruse, K; Harada, N; Ozaki, N; Seino, Y; Shibata, T; Kondo, M; Kato, J; Okawa, T; Fukami, A; Hamada, Y; Inagaki, N; Seino, Y; Drucker, DJ; Oiso, Y; Nakamura, J
      Diabetes  60  2397-406  2011

      Show Abstract
      21810596 21810596
      Exogenous glucagon-like peptide-1 attenuates the glycaemic response to postpyloric nutrient infusion in critically ill patients with type-2 diabetes.
      Adam M Deane,Matthew J Summers,Antony V Zaknic,Marianne J Chapman,Robert J L Fraser,Anna E Di Bartolomeo,Judith M Wishart,Michael Horowitz
      Critical care (London, England)  15  2011

      Show Abstract Full Text Article
      21255422 21255422
      Melatonin stimulates glucagon secretion in vitro and in vivo.
      Ina Bähr,Eckhard Mühlbauer,Helena Schucht,Elmar Peschke
      Journal of pineal research  50  2011

      Show Abstract
      21244480 21244480
      Dynamic modulation of prohormone convertase 2 (PC2)-mediated precursor processing by 7B2 protein: preferential effect on glucagon synthesis.
      Michael Helwig,Sang-Nam Lee,Jae Ryoung Hwang,Akihiko Ozawa,Juan F Medrano,Iris Lindberg
      The Journal of biological chemistry  286  2011

      Show Abstract
      22013069 22013069
      Chronic social defeat stress disrupts regulation of lipid synthesis.
      JC Chuang, H Cui, BL Mason, M Mahgoub, AL Bookout, HG Yu, M Perello, JK Elmquist, JJ Repa, JM Zigman, M Lutter
      Journal of lipid research  2010

      Show Abstract
      20129912 20129912
      Palmitate affects insulin receptor phosphorylation and intracellular insulin signal in a pancreatic alpha-cell line.
      Piro, Salvatore, et al.
      Endocrinology, 151: 4197-206 (2010)  2010

      Show Abstract
      20573722 20573722
      Performance and metabolic and endocrine changes with emphasis on glucose metabolism in high-yielding dairy cows with high and low fat content in liver after calving.
      H M Hammon, G Stürmer, F Schneider, A Tuchscherer, H Blum, T Engelhard, A Genzel, R Staufenbiel, W Kanitz
      Journal of dairy science  92  1554-66  2009

      Show Abstract
      19307636 19307636
      Insulinostatic activity of cerebellin--evidence from in vivo and in vitro studies in rats.
      Strowski MZ, Kaczmarek P, Mergler S, Wiedenmann B, Domin D, Szwajkowski P, Wojciechowicz T, Skrzypski M, Szczepankiewicz D, Szkudelski T, Rucinski M, Malendowicz LK, Nowak KW
      Regulatory peptides  157  19-24  2009

      Show Abstract
      19481574 19481574
      Impact of Sur1 gene inactivation on the morphology of mouse pancreatic endocrine tissue.
      Ihsane Marhfour, Pierre Moulin, Joëlle Marchandise, Jacques Rahier, Christine Sempoux, Yves Guiot
      Cell and tissue research  335  505-15  2009

      Show Abstract
      19142666 19142666

      Brochure

      Title
      A closer look at immunoassay platforms. Beyond fit for purpse. Bring your biomarkers to life with EMD Millipore Immunoassay Platform Solutions (SigmaMillipore)
      ELISAs, RIAs & GyroMark HT Assays
      Protein Detection Platform and Services Brochure

      Technical Info

      Title
      Quality Control Ranges for Catalog # 6000-K Expected Ranges of Each Analyte Using EMD MILLIPORE RIA Kits as Listed Below

      Data Sheet

      Title
      Full Service Custom-built Assays (MilliporeSigma)

      Newsletters / Publications

      Title
      Paper: Roux en Y gastric bypass hypoglycemia resolves with gastric feeding or reversal: Confirming a non-pancreatic etiology

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Radioimmunoassay (RIA) > Complete RIA Kits
      Life Science Research > Antibodies and Assays > Immunoassays > Radioimmunoassay (RIA) > Complete RIA Kits