345749 GDF-8 Propeptide, His•Tag®, Mouse, Recombinant

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345749
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Overview

Replacement Information

Key Specifications Table

Purity
≥90% by SDS-PAGE

Pricing & Availability

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345749-25UG
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      Glass bottle 25 μg
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      Description
      OverviewRecombinant, mouse growth differentiation factor-8 (GDF-8)/myostatin propeptide, consisting of amino acids 25-285, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. GDF-8 is a secreted protein that is expressed during embryonic development and in adult skeletal muscle.
      Catalogue Number345749
      Brand Family Calbiochem®
      SynonymsMyostatin Propeptide
      References
      ReferencesHill, J.J. et al. 2003. Molecular Endocrinology 17, 1144.
      Thies, R.S. et al. 2001. Growth Factors 18, 251.
      Chang, H. et al. 2002. Endocrine Reviews 23, 787.
      McPherron, A.C. et al. 1997. Nature 387, 83.
      Product Information
      FormLyophilized
      FormulationLyophilized from 0.2 µm filtered PBS, BSA.
      Quality LevelMQ100
      Applications
      ApplicationGDF-8 Propeptide, His•Tag®, Mouse, Recombinant, consists of amino acids 25-285, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells.
      Biological Information
      Biological activityED₅₀ = 0.05-0.25 µg/ml (using 40 ng/ml rmGDF-8) as measured by inhibition of rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Approximately 1 µg/ml will completely inhibit GDF-8 activity in K562 cells.
      Purity≥90% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotoxin: ≤1.0 EU/µg protein
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstituyion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      GDF-8 Propeptide, His•Tag®, Mouse, Recombinant SDS

      Title

      Safety Data Sheet (SDS) 

      GDF-8 Propeptide, His•Tag®, Mouse, Recombinant Certificates of Analysis

      TitleLot Number
      345749

      References

      Reference overview
      Hill, J.J. et al. 2003. Molecular Endocrinology 17, 1144.
      Thies, R.S. et al. 2001. Growth Factors 18, 251.
      Chang, H. et al. 2002. Endocrine Reviews 23, 787.
      McPherron, A.C. et al. 1997. Nature 387, 83.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-June-2011 RFH
      SynonymsMyostatin Propeptide
      DescriptionRecombinant, mouse growth differentiation factor-8 (GDF-8)/myostatin propeptide, consisting of amino acids 25-285, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-derived mouse myeloma cells. GDF-8 is a secreted protein that is expressed during embryonic development and in adult skeletal muscle.
      FormLyophilized
      FormulationLyophilized from 0.2 µm filtered PBS, BSA.
      Purity≥90% by SDS-PAGE
      ContaminantsEndotoxin: ≤1.0 EU/µg protein
      Biological activityED₅₀ = 0.05-0.25 µg/ml (using 40 ng/ml rmGDF-8) as measured by inhibition of rmGDF-8 activity in K562 human chronic myelogenous leukemia cells. Approximately 1 µg/ml will completely inhibit GDF-8 activity in K562 cells.
      SolubilityReconstitute to 50 µg/ml with sterile PBS containing ≥1% human or bovine serum albumin.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstituyion refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesHill, J.J. et al. 2003. Molecular Endocrinology 17, 1144.
      Thies, R.S. et al. 2001. Growth Factors 18, 251.
      Chang, H. et al. 2002. Endocrine Reviews 23, 787.
      McPherron, A.C. et al. 1997. Nature 387, 83.