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344145 FPTase, Rat, Recombinant, E. coli

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344145
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344145-100UG
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      Plastic ampoule 100 μg
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      Description
      OverviewRecombinant, rat FPTase expressed in E. coli. A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a variety of cellular proteins including Ras. Recognizes the C-terminal CAAX cysteine motif where the farnesylated cysteine is followed by two aliphatic amino acids and either leucine or serine.
      M.W. α-subunit: ~48,000; β-subunit: ~46,000
      Catalogue Number344145
      Brand Family Calbiochem®
      SynonymsFarnesyl Proteintransferase, FTase
      References
      ReferencesHightower, K.E., et al. 2001. Biochem. J. 360, 625.
      Zimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
      Reiss, Y., et al. 1990. Cell 62, 81.
      Product Information
      FormLiquid
      FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
      Quality LevelMQ200
      Applications
      ApplicationFPTase, Rat, Recombinant, E. coli, a heterodimeric enzyme catalyzes the transfer of a 15-carbon isoprenoid group to a variety of cellular proteins. Recognizes the C-terminal CAAX cysteine motif.
      Biological Information
      Biological activity1 pmol of FPTase will transfer 1 pmol of farnesyl to H-Ras in 15 min at 37°C.
      Purity≥85% by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      FPTase, Rat, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      FPTase, Rat, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      344145

      References

      Reference overview
      Hightower, K.E., et al. 2001. Biochem. J. 360, 625.
      Zimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
      Reiss, Y., et al. 1990. Cell 62, 81.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-October-2010 JSW
      SynonymsFarnesyl Proteintransferase, FTase
      DescriptionRecombinant, rat FPTase expressed in E. coli. A heterodimeric enzyme that catalyzes the transfer of a 15-carbon isoprenoid group to a C-terminal cysteine in a variety of cellular proteins, including Ras. Recognizes the CaaX motif where the farnesylated cysteine (C) is followed by two aliphatic amino acids (aa) and either leucine or serine (X). Expressed in E. coli as an untagged recombinant protein.
      FormLiquid
      FormulationIn 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
      Purity≥85% by SDS-PAGE
      Biological activity1 pmol of FPTase will transfer 1 pmol of farnesyl to H-Ras in 15 min at 37°C.
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesHightower, K.E., et al. 2001. Biochem. J. 360, 625.
      Zimmerman, K.K., et al. 1998. Protein Expr. Purif. 14, 395.
      Reiss, Y., et al. 1990. Cell 62, 81.