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341208 Exoenzyme C3, Clostridium botulinum

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341208
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341208-50UG
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      Plastic ampoule 50 μg
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      Description
      OverviewCatalyzes the hydrolysis of NAD and, in the presence of Rho, catalyzes the ADP-ribosylation of Asn41 in Rho. Inhibits the progression of the cell cycle from G1 to S phase.
      Catalogue Number341208
      Brand Family Calbiochem®
      References
      ReferencesUdagawa, T., and McIntyre, B.W. 1996. J. Biol. Chem. 271, 12542.
      Yamamoto, M., et al. 1994. J. Dermatol. Sci. 8, 103.
      Yamamoto, M., et al. 1993. Oncogene 8, 1449.
      Popoff, A., et al. 1991. Infect. Immun. 59, 3673.
      Morii, N., et al. 1988. J. Biol. Chem. 263, 12420.
      Hartree, E.F. 1972. Anal. Biochem. 48, 422.
      Weber, K., and Osborn, M. 1969. J. Biol. Chem. 244, 4406.
      Product Information
      CAS number58319-92-9
      FormSolid
      FormulationLyophilized from 20 mM Tris, 50 mM NaCl, 0.5% sucrose, 0.1% dextran, pH 7.5
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 37

      Irritating to respiratory system.
      S PhraseS: 36

      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 months at -70°C.
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Exoenzyme C3, Clostridium botulinum Certificates of Analysis

      TitleLot Number
      341208

      References

      Reference overview
      Udagawa, T., and McIntyre, B.W. 1996. J. Biol. Chem. 271, 12542.
      Yamamoto, M., et al. 1994. J. Dermatol. Sci. 8, 103.
      Yamamoto, M., et al. 1993. Oncogene 8, 1449.
      Popoff, A., et al. 1991. Infect. Immun. 59, 3673.
      Morii, N., et al. 1988. J. Biol. Chem. 263, 12420.
      Hartree, E.F. 1972. Anal. Biochem. 48, 422.
      Weber, K., and Osborn, M. 1969. J. Biol. Chem. 244, 4406.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision08-June-2016 RB
      DescriptionCatalyzes the hydrolysis of NAD. Can be used as a reagent for the detection of Rho proteins. Predominantly catalyzes the ADP ribosylation of the Asn41 amino acid residue in Rho. Treatment of cultured cells with exoenzyme C3 has been shown to induce ADP-ribosylation of RhoA protein and to reduce cell growth rate and saturation density. Its growth inhibitory effects appear to result from blockage at the G1 to S phase transition, causing the accumulation of cells in the G1 phase.
      FormSolid
      FormulationLyophilized from 20 mM Tris, 50 mM NaCl, 0.5% sucrose, 0.1% dextran, pH 7.5
      CAS number58319-92-9
      Purity≥90% by SDS-PAGE
      SolubilityReconstitute with sterile distilled H₂O
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 months at -70°C.
      Toxicity Irritant
      ReferencesUdagawa, T., and McIntyre, B.W. 1996. J. Biol. Chem. 271, 12542.
      Yamamoto, M., et al. 1994. J. Dermatol. Sci. 8, 103.
      Yamamoto, M., et al. 1993. Oncogene 8, 1449.
      Popoff, A., et al. 1991. Infect. Immun. 59, 3673.
      Morii, N., et al. 1988. J. Biol. Chem. 263, 12420.
      Hartree, E.F. 1972. Anal. Biochem. 48, 422.
      Weber, K., and Osborn, M. 1969. J. Biol. Chem. 244, 4406.