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115934 Eosin B (bluish) (C.I.45400)

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Key Specifications Table

CAS #EC NumberHill FormulaMolar Mass
548-24-3208-943-1C₂₀H₆Br₂N₂Na₂O₉624.08 g/mol

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      Glass bottle 25 g
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      Catalogue Number115934
      SynonymsDibromodinitrofluorescein sodium
      DescriptionEOSIN B (C.I. 45400) FOR MICROSCOPY CERT
      OverviewEosin B (C.I. 45400) is a CERTISTAIN® dry dye for microscopy that is often used as a counterstain, vizualising proteins, connective tissues, fibers and keratin in sample material of human origin. Depending on user requirements and personal preferences, it can be used for the preparation of the commonly used aqueous or alcoholic Eosin B staining solutions. This staining solution will mostly be used for routine staining in the hematoxylin-eosin staining process (H&E). The H&E staining process is the most frequently used staining method for histological material. Due to the hematoxylin solution, the nuclei will appear blue to dark violet in color and the Eosin Y solution will stain the cytoplasma and intercellular substances red-orange.
      As the dye belongs to the portfolio of Certistain® dyes, it is chemically analyzed according to strict specifications before release (CE marked and IVD product) and a high staining quality, with reliable staining results is guaranteed.
      Product Information
      CAS number548-24-3
      EC number208-943-1
      Hill FormulaC₂₀H₆Br₂N₂Na₂O₉
      Molar Mass624.08 g/mol
      HS Code3204 12 00
      Structure formula ImageStructure formula Image
      Quality LevelMQ400
      ApplicationCertistain® dry dye used for counterstaining
      Biological Information
      Physicochemical Information
      pH value6.2 (10 g/l, H₂O, 25 °C)
      Bulk density680 kg/m3
      Solubility300 g/l
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Storage class10 - 13 Other liquids and solids
      WGKWGK 3 highly hazardous to water
      Relatively unreactive organic reagents should be collected in container A. If halogenated, they should be collected in container B. For solid residues use container C.
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      StorageStore at +2°C to +30°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Dye content (gravimetrically)≥ 85 %
      - sodium

      passes test
      - UV/VIS-Spectrumpasses test
      Absorption maximum λ max (water)517 - 525 nm
      Spec. Absorptivity A 1%/1cm (λmax; 0.005 g/l; water)800 - 1000
      TLC-Testpasses test
      Loss on drying (110 °C)≤ 8 %
      Suitability for microscopypasses test

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      Accessories (Primary)

      Catalog Number Description  
      115935 Eosin Y (yellowish) (C.I. 45380) Show Pricing & Availability

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      IVD/OEM Materials & Reagents > Microscopy > Dyes for Microscopy > Certistain® Dyes
      Reagents, Chemicals and Labware > Inorganic Reagents > Safety Products and General Applications > Indicators > Acid Alkali Indicators or pH Indicators