SCR023 Endothelial Cell Characterization Kit

SCR023
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      Overview

      Replacement Information

      Key Specifications Table

      Key Applications
      ICC
      Description
      Catalogue NumberSCR023
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionEndothelial Cell Characterization Kit
      OverviewEndothelial Cell Characterization Kit

      Antibodies for the immunocytochemical characterization of endothelial cells.
      Background InformationEndothelial cells form the inner lining of blood vessels and serve as the cellular interface between the circulating blood and the vessel wall. Endothelial cells are involved in many aspects of vascular biology, including inflammation, angiogenesis, atherosclerosis, blood clotting, vasoconstriction and vasodilation. Chemicon's endothelial cell characterization kit enables researchers to characterize cultured endothelial cells in both a constitutive and activated state using basic immunocytochemistry techniques.

      Endothelial cells express the following markers constitutively. For optimal results, the following antibody dilutions are recommended for immunocytochemistry:

      * Mouse anti-Endothelial Cell (CD146): 1/500 dilution based on 1 mg/mL, final 2 ng/mL

      * Rabbit anti-VWF: 1/5000 dilution based on 1 mg/mL, final 0.2 ng/mL

      * Mouse anti-CD31: 1/50 dilution based on 0.1 mg/mL, final 20 ng/mL

      The following antibodies have been shown to stain cultured HUVEC after 4-6hr of TNF-alpha (100ng/ml) induction at the following dilutions:

      * Mouse anti-ICAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/mL

      * Mouse anti-VCAM-1: 1/500 dilution based on 1 mg/mL, final 2 ng/Ml
      References
      Product Information
      Components
      • 50 μg of Endothelial Cell (CD146) monoclonal (Part No. 2003789)
      • 50 μg of CD31 monoclonal (Part No. 2003788)
      • 10 μg of von Willibrand Factor (vWF) polyclonal (Part No. 2003787)
      • 50 μg of VCAM-1 monoclonal (Part No.MAB2511-50UG)
      • 50 μg of ICAM-1 monoclonal (Part No. 2003785)
      • 100 μg of Mouse control IgG (Part No. PP54-100UG)
      • 100 μg of Rabbit control IgG (Part No. PP64-100UG)
      Applications
      ApplicationEndothelial cell characterization kits enable researchers to characterize cultured endothelial cells in both a constitutive & activated state using basic ICC techniques.
      Key Applications
      • Immunocytochemistry
      Biological Information
      Species Reactivity
      • Human
      Stem Cell Type
      • Mouse Embryonic Stem Cells
      • Human Embryonic Stem Cells
      • Mesenchymal Stem Cells
      • Neural Stem Cells
      • Hematopoietic Stem Cells
      • Epithelial Cells
      • Pancreatic Stem Cells
      • Cardiac Stem Cells
      • Induced Pluripotent Stem Cells
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsAntibodies to ICAM-1, VCAM-1, von Willibrand Factor (vWF), and Endothelial Cells (CD146) are to be stored at 2 - 8°C (SCR023 - Part 1). Antibodies to CD31, Mouse IgG, and Rabbit IgG are to be stored at -20°C (SCR023 - Part 2).
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Endothelial Cell Characterization Kit SDS

      Title

      Safety Data Sheet (SDS) 

      Endothelial Cell Characterization Kit Certificates of Analysis

      TitleLot Number
      ENDOTHELIAL CELL CHARACTERIZATION KIT2987129
      ENDOTHELIAL CELL CHARACTERIZATION KIT - 21459432145943
      ENDOTHELIAL CELL CHARACTERIZATION KIT - 21884812188481
      ENDOTHELIAL CELL CHARACTERIZATION KIT - 22871842287184
      ENDOTHELIAL CELL CHARACTERIZATION KIT - 32255923225592
      ENDOTHELIAL CELL CHARACTERIZATION KIT -24995952499595
      ENDOTHELIAL CELL CHARACTERIZATION KIT -28019332801933

      Data Sheet

      Title
      ENDOTHELIAL CELL CHARACTERIZATION KIT