324713 Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus

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324713
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324713-50UG
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      Glass bottle 50 μg
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      Description
      OverviewNative endoproteinase Glu-C from Staphylococcus aureus. Serine protease that specifically hydrolyzes peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
      Catalogue Number324713
      Brand Family Calbiochem®
      SynonymsV8 Protease
      References
      ReferencesHoumard, J., and Drapeau, G.R. 1972. Proc. Natl. Acad. Sci. USA 69, 3506.
      Product Information
      CAS number66676-43-5
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-Phe-Leu-Glu-<i>p</i>NA per min at 25°C, pH 7.8.
      EC number3.4.21.19
      FormLyophilized
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥15 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 20-36/37/38-42/43

      Harmful by inhalation.
      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      S PhraseS: 22-26-36

      Do not breathe dust.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus SDS

      Title

      Safety Data Sheet (SDS) 

      Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus Certificates of Analysis

      TitleLot Number
      324713

      References

      Reference overview
      Houmard, J., and Drapeau, G.R. 1972. Proc. Natl. Acad. Sci. USA 69, 3506.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision19-June-2008 RFH
      SynonymsV8 Protease
      DescriptionNative endoproteinase Glu-C from Staphylococcus aureus. Serine protease that specifically hydrolyzes peptide bonds at the carboxylic side of glutamic acid and aspartic acid residues. Inhibited by DFP and α2-macroglobulin.
      FormLyophilized
      Recommended reaction conditions
      Protocol 1. Dissolve the protein to be digested in buffer (e.g. 25 mM ammonium carbonate, pH 7.8). If the protein requires denaturation, add SDS (≤0.1%), guanidine hydrochloride (≤1 M), or urea (≤1 M) to the digestion buffer. (Note: if urea is used, also include 20 mM methylamine to avoid cyanate ion blockage of the N-terminus substrate protein. 2. Suggested working concentration is 1:20-1:100 (protease:protein by weight for sequence analysis). 3. Incubate for 2-18 h at 25°C.
      CAS number66676-43-5
      EC number3.4.21.19
      Specific activity≥15 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-Phe-Leu-Glu-pNA per min at 25°C, pH 7.8.
      SolubilityH₂O
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
      Toxicity Harmful
      ReferencesHoumard, J., and Drapeau, G.R. 1972. Proc. Natl. Acad. Sci. USA 69, 3506.

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      Categories

      Life Science Research > Proteins and Enzymes > Other Enzymes