324725 Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli

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324725
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324725-700MIU
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      Glass bottle 700 miu
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      Description
      OverviewRecombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins.
      Note: 1 mU = 1 milliunit.
      Catalogue Number324725
      Brand Family Calbiochem®
      SynonymsEndo-β-N-acetylglucosaminidase F1, Endo F1
      References
      ReferencesTarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
      Tarentino, A.L., et al. 1992. J. Biol. Chem. 267, 3868.
      Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
      Product Information
      Activity≥17 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5.
      EC number3.2.1.96
      FormLiquid
      FormulationIn 20 mM Tris-HCl, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Specific Activity≥16 units/mg protein
      Physicochemical Information
      ContaminantsProteases: none detected.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Endoglycosidase F1, Elizabethkingia meningosepticum, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      324725

      References

      Reference overview
      Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
      Tarentino, A.L., et al. 1992. J. Biol. Chem. 267, 3868.
      Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.

      Citations

      Title
    • Emily M. Kwan, et al. (2005) N-Glycosidase-carbohydrate-binding module fusion proteins as immobilized enzymes for protein deglycosylation. Protein Engineering Design and Selection 18, 497-501.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-September-2007 RFH
      SynonymsEndo-β-N-acetylglucosaminidase F1, Endo F1
      DescriptionRecombinant, Elizabethkingia meningosepticum endoglycosidase F1 expressed in E. coli. Cleaves asparagine-linked or free oligomannose and hybrid, but not complex oligosaccharides. Core fucosylation reduces activity by 50 fold. Endo F1 will hydrolyze sulfate containing high mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185), and therefore is more suitable for deglycosylation of native proteins.
      FormLiquid
      FormulationIn 20 mM Tris-HCl, pH 7.5.
      Recommended reaction conditions50 mM sodium phosphate buffer, pH 5.5
      EC number3.2.1.96
      ContaminantsProteases: none detected.
      Specific activity≥16 units/mg protein
      Activity≥17 units/ml
      Unit definitionOne unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol denatured ribonuclease B per min at 37°C, pH 5.5.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesTarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
      Tarentino, A.L., et al. 1992. J. Biol. Chem. 267, 3868.
      Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
      Citation
    • Emily M. Kwan, et al. (2005) N-Glycosidase-carbohydrate-binding module fusion proteins as immobilized enzymes for protein deglycosylation. Protein Engineering Design and Selection 18, 497-501.