Key Specifications Table
|RIP, Protein Interaction Assays|
|Description||EZ-Magna Nuclear RIP™ (Native)
Nuclear RNA-Binding Protein Immunoprecipitation Kit
|Overview||Features and Advantages
Magna Nuclear RIP™ Kits are specially designed to allow the discovery and analysis of a variety of chromatin associated RNAs such as long non-coding RNAs, enhancer RNAs and miRNAs . These chromatin-associated RNAs often regulate gene expression and can be analyzed with applications including quantitative reverse transcription polymerase chain reaction (RT-PCR), microarray analysis (RIP-chip) and next generation sequencing (RIP-Seq).
Nuclear RIP can be performed using chromatin that has interactions stabilized by formaldehyde treatment (cross-linked) or chromatin that has not been treated with a cross linking reagent (native). While both of these approaches are similar in that they are designed to recover chromatin associated RNA, the reagents used and the details of the protocol and types of interactions typically detected are different. Native RIP is expected to recover high affinity, more direct interactions between proteins encoded RNA binding motifs and candidate RNAs. In contrast, nuclear RIP with cross-linked chromatin can capture higher molecular weight complexes in in vivo configurations with possibly lower affinities. For less well understood proteins and protein complexes often both approaches are used.
The kit described here is for the native approach. If a cross-linked approach is of interest please visit the product page for the Magna Nuclear RIP
(Cross-Linked) Kit , catalogue # 17-10520 or the EZ-Magna Nuclear RIP (Cross-Linked) Kit, catalogue # 17-10521.
|Materials Required but Not Delivered||Reagents
|Presentation||Two boxes containing key reagents for generation of native nuclear lysates and performance of 24 individual RNA-binding protein immunoprecipitation (RIP) reactions. Plus box containing positive and negative control antibodies and qPCR primers.|
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||Upon receipt, store components at the temperatures indicated on the labels.
Kit components are stable for 6 months from date of shipment when stored as directed.
|Material Size||24 assays|
|Material Package||Kit capacity: 24 RNA-binding protein immunoprecipitation assays using a native (non-cross-linked) nuclear lysate|
|White Paper - The Message in the Marks: Deciphering Cancer Epigenetics|
|Magna Nuclear RIP¿ (Native) Nuclear RNA-Binding Protein Immunoprecipitation Kit|