|EZ- Magna ChIRP RNA Interactome Kit -Isolation and characterization of non-coding RNA:chromatin complexes
|Features and Advantages
The Magna ChIRP RNA Interactome Kit uses the ChIRP method (Chromatin Isolation by RNA Purification) to enable identification and analysis of regions of chromatin that interact with a particular chromatin-associated RNA. This novel anti-sense probe-based capture strategy uses cross-linked chromatin to allow unbiased discovery of RNA-associated DNA sequences and proteins. To perform ChIRP two pools consisting of mulitple biotinylated oligonucleotide probes complementary to the RNA of interest are used (see figure 1). These probe sets are combined with chromatin and hybridized to the chromatin-associated RNA. Complexes containing biotinylated-probes bound to the chromatin-associated RNA are then isolated using streptavidin magnetic beads. DNA or RNA can then be recovered and analyzed by quantitative PCR or next generation sequencing (ChIRP-seq). Alternatively, proteomic analysis of the recovered chromatin can be performed.
Learn more about the ChIRP method in JOVE! (Watch Now!)
Need help making your own ChIRP probe sets? (Use the ChIRP probe designer tool.)
|Materials Required but Not Delivered
|Three boxes containing key reagents for the extraction of ChIRP ready chromatin, plus magnetic beads, enzymes, buffers, biotinlyated negative control probes, biotinylated TERC odd and even positive control probe set and PCR primers to allow the performance of 12 chromatin isolation by RNA purification assays.
|Safety Information according to GHS
|Storage and Shipping Information
|Upon receipt, store components at the temperatures indicated on the labels.
Kit components are stable for 6 months from date of shipment when stored as directed.
|Kit capacity: 12 Chromatin Isolation by RNA Purification Assays (includes negative control probes, even and odd positive control TERC lncRNA probes, and control PCR primer sets)
|Global Trade Item Number
EZ- Magna ChIRP RNA Interactome Kit -Isolation and characterization of non-coding RNA:chromatin complexes SDS
|Pub Med ID
|Long noncoding RNA BCAR4 promotes osteosarcoma progression through activating GLI2-dependent gene transcription.
Chen, F; Mo, J; Zhang, L
Tumour Biol 13403-13412 2016
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