|Description||EZ- Magna ChIRP RNA Interactome Kit -Isolation and characterization of non-coding RNA:chromatin complexes|
|Overview||Features and Advantages
The Magna ChIRP RNA Interactome Kit uses the ChIRP method (Chromatin Isolation by RNA Purification) to enable identification and analysis of regions of chromatin that interact with a particular chromatin-associated RNA. This novel anti-sense probe-based capture strategy uses cross-linked chromatin to allow unbiased discovery of RNA-associated DNA sequences and proteins. To perform ChIRP two pools consisting of mulitple biotinylated oligonucleotide probes complementary to the RNA of interest are used (see figure 1). These probe sets are combined with chromatin and hybridized to the chromatin-associated RNA. Complexes containing biotinylated-probes bound to the chromatin-associated RNA are then isolated using streptavidin magnetic beads. DNA or RNA can then be recovered and analyzed by quantitative PCR or next generation sequencing (ChIRP-seq). Alternatively, proteomic analysis of the recovered chromatin can be performed.
Learn more about the ChIRP method in JOVE! (Watch Now!)
Need help making your own ChIRP probe sets? (Use the ChIRP probe designer tool.)
|Materials Required but Not Delivered||Reagents
|Safety Information according to GHS|
|Storage and Shipping Information|
|Storage Conditions||Upon receipt, store components at the temperatures indicated on the labels.
Kit components are stable for 6 months from date of shipment when stored as directed.
|Material Size||12 Assays|
|Material Package||Kit capacity: 12 Chromatin Isolation by RNA Purification Assays (includes negative control probes, even and odd positive control TERC lncRNA probes, and control PCR primer sets)|
|Magna ChIRP™ RNA Interactome Kits|
|NPI Flyer- Epigenetics and Nuclear Function Feature (EMD)|
|New Products - Antibodies, Small Molecule, Inhibitors|
|White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)|
|White Paper: Further considerations of antibody validation and usage. (EMD)|
|Analysis of Long Noncoding RNA Chromatin Interactions by Chromatin Isolation by RNA Purification (ChIRP)|
Instructions for Use
|EZ- Magna ChIRP RNA Interactome Kit Analysis and Mapping of Chromatin: Noncoding RNA Interactions|