322327 Diphtheria Toxin, CRM Mutant - CAS 92092-36-9 - Calbiochem

Diphtheria Toxin, CRM197 Mutant, CAS 92092-36-9, is a nontoxic material with a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
322327
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      Overview

      Replacement Information

      Key Specifications Table

      CAS #
      92092-36-9

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      322327-1MG
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          		 Plastic ampoule 1 mg
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          Description
          OverviewCRM197 is a nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity. Does not catalyze the ADP ribosylation of EF-2 factor. Immunologically, the CRM197 protein is identical to the wild-type toxin.
          Catalogue Number322327
          Brand Family Calbiochem®
          References
          ReferencesKusano, I., et al. 2001. Cell Struct. Funct. 26, 279.
          Kaefer, M., et al. 2000. J. Urol. 163, 580.
          Gupta, R.K., et al. 1997. Vaccine 15, 1341.
          Bixler, G.S., et al. 1989. Adv. Exp. Med. Biol. 251, 175.
          Giannini, G., et al. 1984. Nucleic Acids Res. 12, 4063.
          Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
          Pappenheimer, Jr., A.M., et al. 1972. Immunochem. 9, 891.
          Product Information
          CAS number92092-36-9
          FormSolid
          FormulationLyophilized from 10 mM sodium phosphate buffer, 5% lactose, pH 7.4.
          ReversibleN
          Quality LevelMQ100
          Applications
          Biological Information
          Primary TargetMutant with loss of enzymatic activity to catalyze the ADP ribosylation of EF-2 factor
          PurityOne major band by SDS-PAGE
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 year at -70°C.
          Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Diphtheria Toxin, CRM Mutant - CAS 92092-36-9 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Diphtheria Toxin, CRM Mutant - CAS 92092-36-9 - Calbiochem Certificates of Analysis

          TitleLot Number
          322327

          References

          Reference overview
          Kusano, I., et al. 2001. Cell Struct. Funct. 26, 279.
          Kaefer, M., et al. 2000. J. Urol. 163, 580.
          Gupta, R.K., et al. 1997. Vaccine 15, 1341.
          Bixler, G.S., et al. 1989. Adv. Exp. Med. Biol. 251, 175.
          Giannini, G., et al. 1984. Nucleic Acids Res. 12, 4063.
          Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
          Pappenheimer, Jr., A.M., et al. 1972. Immunochem. 9, 891.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-February-2011 JSW
          DescriptionCross-reactive material of diphtheria toxin (CRM197). A nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A that results in complete loss of enzymatic activity. Inhibits smooth muscle cell proliferation in a dose-dependent manner. Immunologically, the CRM197 is identical to the wild-type toxin and is useful as a carrier protein of conjugate vaccines.
          FormSolid
          FormulationLyophilized from 10 mM sodium phosphate buffer, 5% lactose, pH 7.4.
          CAS number92092-36-9
          PurityOne major band by SDS-PAGE
          SolubilityPlease refer to vial label for lot-specific reconstitution volume.
          Storage +2°C to +8°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 year at -70°C.
          Toxicity Standard Handling
          ReferencesKusano, I., et al. 2001. Cell Struct. Funct. 26, 279.
          Kaefer, M., et al. 2000. J. Urol. 163, 580.
          Gupta, R.K., et al. 1997. Vaccine 15, 1341.
          Bixler, G.S., et al. 1989. Adv. Exp. Med. Biol. 251, 175.
          Giannini, G., et al. 1984. Nucleic Acids Res. 12, 4063.
          Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
          Pappenheimer, Jr., A.M., et al. 1972. Immunochem. 9, 891.