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322327 Diphtheria Toxin, CRM Mutant - CAS 92092-36-9 - Calbiochem

Diphtheria Toxin, CRM197 Mutant, CAS 92092-36-9, is a nontoxic material with a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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322327
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Overview

Replacement Information

Key Specifications Table

CAS #
92092-36-9

Pricing & Availability

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322327-1MG
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      		 Plastic ampoule 1 mg
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      Description
      OverviewCRM197 is a nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity. Does not catalyze the ADP ribosylation of EF-2 factor. Immunologically, the CRM197 protein is identical to the wild-type toxin.
      Catalogue Number322327
      Brand Family Calbiochem®
      References
      ReferencesKusano, I., et al. 2001. Cell Struct. Funct. 26, 279.
      Kaefer, M., et al. 2000. J. Urol. 163, 580.
      Gupta, R.K., et al. 1997. Vaccine 15, 1341.
      Bixler, G.S., et al. 1989. Adv. Exp. Med. Biol. 251, 175.
      Giannini, G., et al. 1984. Nucleic Acids Res. 12, 4063.
      Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
      Pappenheimer, Jr., A.M., et al. 1972. Immunochem. 9, 891.
      Product Information
      CAS number92092-36-9
      FormSolid
      FormulationLyophilized from 10 mM sodium phosphate buffer, 5% lactose, pH 7.4.
      ReversibleN
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetMutant with loss of enzymatic activity to catalyze the ADP ribosylation of EF-2 factor
      PurityOne major band by SDS-PAGE
      Physicochemical Information
      Cell permeableN
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 year at -70°C.
      Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Diphtheria Toxin, CRM Mutant - CAS 92092-36-9 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Diphtheria Toxin, CRM Mutant - CAS 92092-36-9 - Calbiochem Certificates of Analysis

      TitleLot Number
      322327

      References

      Reference overview
      Kusano, I., et al. 2001. Cell Struct. Funct. 26, 279.
      Kaefer, M., et al. 2000. J. Urol. 163, 580.
      Gupta, R.K., et al. 1997. Vaccine 15, 1341.
      Bixler, G.S., et al. 1989. Adv. Exp. Med. Biol. 251, 175.
      Giannini, G., et al. 1984. Nucleic Acids Res. 12, 4063.
      Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
      Pappenheimer, Jr., A.M., et al. 1972. Immunochem. 9, 891.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-February-2011 JSW
      DescriptionCross-reactive material of diphtheria toxin (CRM197). A nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A that results in complete loss of enzymatic activity. Inhibits smooth muscle cell proliferation in a dose-dependent manner. Immunologically, the CRM197 is identical to the wild-type toxin and is useful as a carrier protein of conjugate vaccines.
      FormSolid
      FormulationLyophilized from 10 mM sodium phosphate buffer, 5% lactose, pH 7.4.
      CAS number92092-36-9
      PurityOne major band by SDS-PAGE
      SolubilityPlease refer to vial label for lot-specific reconstitution volume.
      Storage +2°C to +8°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 year at -70°C.
      Toxicity Standard Handling
      ReferencesKusano, I., et al. 2001. Cell Struct. Funct. 26, 279.
      Kaefer, M., et al. 2000. J. Urol. 163, 580.
      Gupta, R.K., et al. 1997. Vaccine 15, 1341.
      Bixler, G.S., et al. 1989. Adv. Exp. Med. Biol. 251, 175.
      Giannini, G., et al. 1984. Nucleic Acids Res. 12, 4063.
      Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
      Pappenheimer, Jr., A.M., et al. 1972. Immunochem. 9, 891.