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500509 DNA Methyltransferase Inhibitor V, RSC133 - Calbiochem

A cell-permeable compound that targets Dnmt1 at the cofactor SAM-binding site and enhances the efficiency of iPSC production from both murine MEF and human foreskin fibroblasts.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: (E)-3-(3-(1H-Indol-3-yl)acrylamido)benzamide, ID133, iPSC Induction Enhancer III, Reprogramming Stimulating Compound 133, DNA MTase Inhibitor V

Primary Target: Dnmt1
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical Formula
      C₁₈H₁₅N₃O₂
      Description
      OverviewA cell-permeable indolylacrylamido-benzamide compound that is reported to target Dnmt1 at the cofactor SAM-binding site and enhance the efficiency of iPSC production from both murine MEF (10 µM starting 5 d post KSOM viral infection) and human foreskin fibroblast (10 µM starting same day as or 5 d post KSOM viral infection) cultures with accompanying cellular Dnmt1 activity reduction, affecting hFF viability only at high concentrations (78% of control post 72 h 100 µM treatment). Also shown to help maintain H9 hESCs in undifferentiated state when cultured in unconditioned media (10 µM).

      Please note that the molecular weight for this compound is batch-specific due to variable water content. Please refer to the vial label or the certificate of analysis for the batch-specific molecular weight. The molecular weight provided represents the baseline molecular weight without water.
      Catalogue Number500509
      Brand Family Calbiochem®
      Synonyms(E)-3-(3-(1H-Indol-3-yl)acrylamido)benzamide, ID133, iPSC Induction Enhancer III, Reprogramming Stimulating Compound 133, DNA MTase Inhibitor V
      References
      ReferencesLee, J., et al. 2012. Angew. Chem. Int. Engl. 51, 12509.
      Product Information
      FormLight yellow powder
      Hill FormulaC₁₈H₁₅N₃O₂
      Chemical formulaC₁₈H₁₅N₃O₂
      ReversibleY
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetDnmt1
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Identification Number
      Catalog Number GTIN
      500509

      Documentation

      DNA Methyltransferase Inhibitor V, RSC133 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Lee, J., et al. 2012. Angew. Chem. Int. Engl. 51, 12509.

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-January-2014 JSW
      Synonyms(E)-3-(3-(1H-Indol-3-yl)acrylamido)benzamide, ID133, iPSC Induction Enhancer III, Reprogramming Stimulating Compound 133, DNA MTase Inhibitor V
      DescriptionA cell-permeable indolylacrylamido-benzamide compound that is reported to target Dnmt1 at the cofactor SAM-(S-adenosyl methionine) binding site and enhance the efficiency of iPSC production from both murine MEF (by 2.1-fold at 15 d post viral KOSM transduction with 10 µM RSC133 starting 5 d post viral infection) and human foreskin fibroblast (by 3.5- and 2.9-fold 15 d post viral KOSM transduction with 10 µM RSC133 starting same day as or 5 d post viral infection, respectively) cultures with accompanying cellular Dnmt1 activity reduction (by 38% lower in 15 d viral-transduced hiPSC cultures with 10 µM RSC133 starting on d 5 post viral infection), affecting hFF viability only at high concentrations (102% and 78% of control after 72 h incubation with 50 and 100 µM RSC133, respectively, by MTT assays). Also shown to help maintain H9 hESCs in undifferentiated state when cultured in unconditioned media (17% vs 53% drop in AP+ colonies in 6 d UM cultures, respectively, with or without 10 µM RSC133).
      FormLight yellow powder
      Chemical formulaC₁₈H₁₅N₃O₂
      Structure formulaStructure formula
      Purity≥98% by HPLC
      SolubilityDMSO (100 mg/ml). Use only fresh DMSO for reconstitution.
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesLee, J., et al. 2012. Angew. Chem. Int. Engl. 51, 12509.