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251760 DAR-4M - Calbiochem

Overview

Replacement Information

Key Specifications Table

Empirical Formula
C₂₅H₂₆N₄O₃

Pricing & Availability

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251760-1MG
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      Plastic ampoule 1 mg
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      Description
      OverviewA rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of O2, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. The fluorescence intensity is not dependent on pH, unlike DAF-2 (Cat. No. 251500).
      Catalogue Number251760
      Brand Family Calbiochem®
      SynonymsDiaminorhodamine-4M
      References
      ReferencesKojima, H., et al. 2001. Anal. Chem. 73, 1967.
      Kojima, H., et al. 2000. Tetrahedron Lett. 41, 69.
      Product Information
      ATP CompetitiveN
      DeclarationNot available for sale in Japan.
      FormRed violet solution
      FormulationIn DMSO.
      Hill FormulaC₂₅H₂₆N₄O₃
      Chemical formulaC₂₅H₂₆N₄O₃
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetNitric oxide (NO) fluorescent indicator
      Purity≥98% by HPLC
      Physicochemical Information
      Cell permeableN
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 23-26-36

      Do not breathe fumes.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      DAR-4M - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      DAR-4M - Calbiochem Certificates of Analysis

      TitleLot Number
      251760

      References

      Reference overview
      Kojima, H., et al. 2001. Anal. Chem. 73, 1967.
      Kojima, H., et al. 2000. Tetrahedron Lett. 41, 69.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-December-2018 JSW
      SynonymsDiaminorhodamine-4M
      DescriptionA rhodamine-based photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~10 nM. Reacts with NO, in the presence of oxygen, resulting in a triazolo-rhodamine analog (DAR-4M T) that exhibits about 840-fold greater fluorescence quantum efficiency. Unlike DAF-2 (Cat. No. 251500), its fluorescence intensity is not dependent on the pH. Excitation maximum: ~560 nm; emission maximum: ~575 nm.
      FormRed violet solution
      FormulationIn DMSO.
      Recommended reaction conditionsThis reagent is provided as a solution at 5 mM concentration. Prepare a 500-fold dilution in 0.1 M phosphate buffer, pH 7.4 before use. BSA and phenol-red may affect fluorescence.
      Chemical formulaC₂₅H₂₆N₄O₃
      Structure formulaStructure formula
      Purity≥98% by HPLC
      Storage -20°C
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze at (-20°C). Use immediately following dilution and discard any unused portion.
      Toxicity Irritant
      ReferencesKojima, H., et al. 2001. Anal. Chem. 73, 1967.
      Kojima, H., et al. 2000. Tetrahedron Lett. 41, 69.