508741 DAPI Stain - CAS 28718-90-3 - Calbiochem

508741
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Key Specifications Table

Empirical FormulaCAS #
C₁₆H₁₅N₅ • 2HCl 28718-90-3

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      Glass bottle 5 mg
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      Description
      OverviewCells are typically stained at 1 µg/ml.
      Catalogue Number508741
      Brand Family Calbiochem®
      Synonyms4ʹ,6-Diamidino-2-phenylindole, 2HCl, 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine, 2HCl, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide, 2HCl, DAPI, ≥97% by HPLC, in H₂O
      References
      ReferencesMathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
      Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
      Hotz, M.A., et al. 1994. Cytometry 15, 237.
      Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
      Miller, T., et al. 1993. Biotechniques 15, 1042.
      Gray, J.W., et al. 1982. Science 238, 323.
      Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
      Russels, W.C., et al. 1975. Nature 253, 461.
      Product Information
      CAS number28718-90-3
      FormLiquid
      FormulationA 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl (Cat. No. 268298) in H₂O.
      Hill FormulaC₁₆H₁₅N₅ • 2HCl
      Chemical formulaC₁₆H₁₅N₅ • 2HCl
      ReversibleY
      Structure formula ImageStructure formula Image
      Quality LevelMQ200
      Applications
      Biological Information
      Primary TargetDNA
      Purity≥97% by HPLC
      Physicochemical Information
      Cell permeableY
      Emission max.
      Excitation max.
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      DAPI Stain - CAS 28718-90-3 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Mathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
      Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
      Hotz, M.A., et al. 1994. Cytometry 15, 237.
      Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
      Miller, T., et al. 1993. Biotechniques 15, 1042.
      Gray, J.W., et al. 1982. Science 238, 323.
      Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
      Russels, W.C., et al. 1975. Nature 253, 461.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision28-March-2014 JSW
      Synonyms4ʹ,6-Diamidino-2-phenylindole, 2HCl, 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine, 2HCl, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide, 2HCl, DAPI, ≥97% by HPLC, in H₂O
      DescriptionCell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
      FormLiquid
      FormulationA 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl (Cat. No. 268298) in H₂O.
      Intert gas (Yes/No) Packaged under inert gas
      CAS number28718-90-3
      Chemical formulaC₁₆H₁₅N₅ • 2HCl
      Structure formulaStructure formula
      Purity≥97% by HPLC
      Storage -20°C
      Protect from light
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesMathieu, C., et al. 1995. Exp. Cell Res. 216, 13.
      Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.
      Hotz, M.A., et al. 1994. Cytometry 15, 237.
      Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.
      Miller, T., et al. 1993. Biotechniques 15, 1042.
      Gray, J.W., et al. 1982. Science 238, 323.
      Hessling, J.J., et al. 1980. J. Immunol. Methods 38, 315.
      Russels, W.C., et al. 1975. Nature 253, 461.