Key Specifications Table
|Overview||A cell-permeable 5-bromobarbiturate that selectively and covalently modifies the pleckstrin homology (PH) domain of the cytohesin family (Kd ~ 2 µM to cytohesin-1 labeled with Alexa647) and irreversibly inhibits its function. Prevents the interaction cytohesin-1 PH, and to cytohesins 1 and 2 lacking the short C-terminal polybasic region with PIP3 and PIP2. Blocks insulin-dependent cytohesin-2-GFP translocation to plasma membrane in HeLa cells (~50 µM). Does not recognize or show any binding to T-lymphoma invasive and metastasis inducing protein 1 (Tiam)-DH-PH and shows poor affinity towards Sec7 domain of cytohesin-2. Cytohesins Inhibitor SecinH3 is also available (Cat. No. 565725).
Please note that the molecular weight for this compound is batch-specific due to variable water content.
|Synonyms||5-Benzyl-5-bromo-1-(4-chlorophenyl)pyrimidine-2,4,6(1H,3H,5H)-trione, Cytohesin pleckstrin homology domain inhibitor 2, Cyth PH Domain Inhibitor|
|References||Hussein, M., et al. 2013. Angew. Chem. Int. Ed., 52, 9529.|
|Primary Target||pleckstrin homology domain of the cytohesin family|
|Purity||≥97% by HPLC|
|Safety Information according to GHS|
|Product Usage Statements|
|Packaged under inert gas||Packaged under inert gas|
Cytohesin Inhibitor II, Cyplecksin 2 - Calbiochem SDS
|Hussein, M., et al. 2013. Angew. Chem. Int. Ed., 52, 9529.|
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.