Millipore Sigma Vibrant Logo

S8001U CpGenome™ Human Non-Methylated DNA Standard Set

View Products on Sigmaaldrich.com
S8001U
5 µg   
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information
      Description
      Catalogue NumberS8001U
      Brand Family Upstate
      Trade Name
      • CpGenome
      • Upstate
      DescriptionCpGenome™ Human Non-Methylated DNA Standard Set
      OverviewThe CpGenome™ Human Non-Methylated DNA Standard is purified from HCT116 DKO cells that contain genetic knockouts of DNA methyltransferases, DNMT1 (-/-) and DNMT3b (-/-) , and have less than 5% methylated DNA. It is intended for use as a negative control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome™ Turbo Bisulfite Modification Kit (Cat. No. S7847). Bisulfite-modified DNA can be evaluated by methylation-specific PCR (MSP) technology using the CpG WIZ® amplification kits.

      Materials Provided:

      One vial containing 5 µg (20 µL) of CpGenome™ Human Non-Methylated DNA Standard at a concentration of 250 ng/µL.

      Validation:

      Methylation-specific PCR (MSP) was performed on the CpGenome™ Human Non-Methylated DNA after bisulfite modification with the CpGenome™ Turbo Bisulfite kit (Cat. No. S7847). Three sets of primers from the CpG WIZ® BRCA1 amplification kit (Cat. No. S7830) were used in this assay: the U primer set, which anneals to unmethylated bisulfite-modified DNA; M primers, which anneal to methylated bisulfite-modified sequences; and W primers, which anneal to unmethylated or methylated DNA that has not undergone bisulfite modification. Only the U primers generated product for this Standard.

      CpGenome and CpG WIZ are trademarks of Serologicals Corporation. CpG WIZ Methylation Products apply to technologies exclusively licensed from The Johns Hopkins University School of Medicine. Methylation-specific PCR (MSP) technology is covered under U.S. Patent # 5,786,146.
      References
      Product Information
      PresentationLiquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0
      Quality LevelMQ100
      Applications
      ApplicationIt is intended for use as a negative control in gene methylation studies, such as bisulfite conversion of DNA with the CpGenome Turbo Bisulfite Modification Kit.
      Application NotesFor MSP primer design, please use the MethPrime software package. Click here
      Biological Information
      Species Reactivity
      • Human
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended Storage: Stable at -20°C for up to 6 months from date of receipt.
      Packaging Information
      Material Size5 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      CpGenome™ Human Non-Methylated DNA Standard Set SDS

      Title

      Safety Data Sheet (SDS) 

      CpGenome™ Human Non-Methylated DNA Standard Set Certificates of Analysis

      TitleLot Number
      CpGenome Human Non-Methylated DNA Standard - Q2023993 Q2023993
      CpGenome™ Human Non-Methylated -2571269 2571269
      CpGenome™ Human Non-Methylated -2802796 2802796
      CpGenome™ Human Non-Methylated DNA Standard 2984242
      CpGenome™ Human Non-Methylated DNA Standard 3010026
      CpGenome™ Human Non-Methylated DNA Standard 3050833
      CpGenome™ Human Non-Methylated DNA Standard 3126657
      CpGenome™ Human Non-Methylated DNA Standard 2930483
      CpGenome™ Human Non-Methylated DNA Standard - 3156112 3156112
      CpGenome™ Human Non-Methylated DNA Standard - 3189686 3189686

      Brochure

      Title
      Shaping Epigenetics Discovery - DNA Methylation Brochure (MilliporeSigma)

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)