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17-10451 CpGenome Direct Prep Bisulfite Modification Kit (50 Reactions)

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17-10451
50 reactions  
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      Overview

      Replacement Information
      Description
      Catalogue Number17-10451
      ReplacesReplaces S7820 (CpGenome Universal DNA Modification Kit).
      Trade Name
      • CpGenome
      DescriptionCpGenome Direct Prep Bisulfite Modification Kit (50 Reactions)
      OverviewUnlike other bisulfite modifications approaches that start with isolated genomic DNA, the CpGenome Direct Prep Bisulfite Modification Kit allows simple and reliable bisulfite conversion of DNA directly from a variety of starting materials, including cultured cells, blood, fresh tissue and fixed tissue samples.

      Key Features
      • Bisulfite conversion directly from cells, tissues, blood and FFPE samples without DNA purification
      • Sensitive bisulfite-conversion from as few as 10 cells or as low as 50 pg of input DNA
      • Fast and simple, streamlined protocol for one-step bisulfite conversion
      • In-column desulfonation allows recovery of DNA without additional precipitation steps for more consistent results
      • Suitable for downstream analysis by methlylation specific PCR, restriction digestion, sequencing, microarray hybridization, etc.
      Background InformationMethylation of cytosines located 5' to guanosine is known to have a profound effect on the expression of many eukaryotic genes. In normal cells methylation occurs predominantly in CG-poor regions, while CG-rich areas, called CpG-islands remain unmethylated. The exceptions are the extensive methylation of CpG islands associated with transcriptional inactivation of regulatory regions of imprinted genes and genes on the inactive X-chromosome of females. Aberrant methylation of normally unmethylated CpG islands has been documented as a relatively frequent event in immortalized and transformed cells and has been associated with transcriptional inactivation of defined tumor suppressor genes in human cancers. Hundreds of CpG islands are now known to exhibit the characteristic of hypermethylation in tumors resulting in identification of candidate genes that can be interrogated to determine extent of tumor specific transformation.
      Materials Required but Not Delivered• MILLIPLEX MAP Cell Signaling Buffer and Detection Kit (Catalog #48-602)
      References
      Product Information
      Components
      • 2X Extraction Buffer
      • Modification Reagent
      • Conversion Buffer
      • Resuspension Buffer
      • Equilibration Buffer
      • Binding Buffer
      • Wash Buffer I
      • Wash Buffer II
      • Elution Buffer
      • Proteinase K
      • Proteinase K Storage Buffer
      • Desalting Columns
      • Collection Tubes
      Quality LevelMQ100
      Applications
      ApplicationThe CpGenome Direct Prep Bisulfite Modification Kit allows bisulfite conversion directly from a variety of starting materials, including cultured cells, blood, fresh tissue & fixed tissue samples.
      Application NotesFor MSP primer design, please use the MethPrime software package. Click here
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size50 reactions
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      References

      Reference overviewPub Med ID
      A mouse model of Angelman syndrome imprinting defects.
      Lewis, MW; Vargas-Franco, D; Morse, DA; Resnick, JL
      Hum Mol Genet  0  1-10  2018

      Show Abstract
      30260400 30260400
      Genetic and epigenetic alterations in normal and sensitive COPD-diseased human bronchial epithelial cells repeatedly exposed to air pollution-derived PM2.5.
      Leclercq, B; Platel, A; Antherieu, S; Alleman, LY; Hardy, EM; Perdrix, E; Grova, N; Riffault, V; Appenzeller, BM; Happillon, M; Nesslany, F; Coddeville, P; Lo-Guidice, JM; Garçon, G
      Environ Pollut  230  163-177  2017

      Show Abstract
      28651088 28651088
      FHIT promoter DNA methylation and expression analysis in childhood acute lymphoblastic leukemia.
      Bahari, G; Hashemi, M; Naderi, M; Sadeghi-Bojd, S; Taheri, M
      Oncol Lett  14  5034-5038  2017

      Show Abstract
      29085517 29085517
      In vitro Homeostatic Proliferation of Human CD8 T Cells.
      Abdelsamed, HA; Zebley, CC; Youngblood, B
      Bio Protoc  7  1-13  2017

      Show Abstract
      29226183 29226183
      Clinicopathologic Characterization of Aggressive Natural Killer Cell Leukemia Involving Different Tissue Sites.
      Gao, LM; Zhao, S; Liu, WP; Zhang, WY; Li, GD; Küçük, C; Hu, XZ; Chan, WC; Tang, Y; Ding, WS; Yan, JQ; Yao, WQ; Wang, JC
      Am J Surg Pathol  40  836-46  2016

      Show Abstract
      26975038 26975038
      Differential responses of healthy and chronic obstructive pulmonary diseased human bronchial epithelial cells repeatedly exposed to air pollution-derived PM4.
      Leclercq, B; Happillon, M; Antherieu, S; Hardy, EM; Alleman, LY; Grova, N; Perdrix, E; Appenzeller, BM; Lo Guidice, JM; Coddeville, P; Garçon, G
      Environ Pollut  218  1074-1088  2016

      Show Abstract
      27593349 27593349
      Pygopus-2 promotes invasion and metastasis of hepatic carcinoma cell by decreasing E-cadherin expression.
      Zhang, S; Li, J; Liu, P; Xu, J; Zhao, W; Xie, C; Yin, Z; Wang, X
      Oncotarget  6  11074-86  2015

      Show Abstract
      25871475 25871475
      Effects of 5-aza-2'deoxycytidine on RECK gene expression and tumor invasion in salivary adenoid cystic carcinoma.
      Zhou, XQ; Huang, SY; Zhang, DS; Zhang, SZ; Li, WG; Chen, ZW; Wu, HW
      Braz J Med Biol Res  48  254-60  2015

      Show Abstract
      25517920 25517920
      Promoter hypermethylation of the cysteine protease RECK may cause metastasis of osteosarcoma.
      Wang, L; Ge, J; Ma, T; Zheng, Y; Lv, S; Li, Y; Liu, S
      Tumour Biol  36  9511-6  2015

      Show Abstract
      26130413 26130413
      Epigallocatechin‑3‑gallate inhibits the invasion of salivary adenoid cystic carcinoma cells by reversing the hypermethylation status of the RECK gene.
      Zhou, XQ; Xu, XN; Li, L; Ma, JJ; Zhen, EM; Han, CB
      Mol Med Rep  12  6031-6  2015

      Show Abstract
      26299812 26299812

      Brochure

      Title
      CpGenome™ Direct Prep Bisulfite Modification Kit
      Shaping Epigenetics Discovery - DNA Methylation Brochure (MilliporeSigma)

      Technical Info

      Title
      White Paper - The Message in the Marks: Deciphering Cancer Epigenetics (EMD)

      User Guides

      Title
      CpGenome¿Direct Prep Bisulfite Modification Kit