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17-604 ChIPAb+ LEF1 - ChIP Validated Antibody and Primer Set

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17-604
25 assays  25 assays per kit. ~4μg per chromatin immunoprecipitation.
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      H, MWB, ChIP
      Description
      Catalogue Number17-604
      Brand Family Upstate
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+ LEF1 - ChIP Validated Antibody and Primer Set
      OverviewEvery lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. LEF1 antibody and the negative control antibody (mouse normal IgG) can be used to demonstrate that the LEF1 antibody is functionally validated in the precipitation of LEF1 associated chromatin.
      The qPCR primers included flank the LEF1/TCF binding site in human c-myc promoter.
      References
      Product Information
      FormatPurified
      Control
      • Includes negative control mouse IgG antibody and control primers specific for human c-myc promoter.
      PresentationAnti-LEF1 (mouse IgG1). 1 vial containing 100 mg of thiophilic and size exclusion chromatography purified mouse IgG1 in 100 mL of phosphate buffered saline with sodium azide. Store at-20°C. The antibody is made against amino acid residues 1-85 of human LEF1 and can recognize human and mouse LEF1. It does not cross-react with TCF-3 or TCF-4.

      Normal Mouse IgG. One vial containing 125 μg of normal mouse IgG in 125 μL volume. Store at -20°C.

      ChIP primers c-myc. One vial containing 75 mL of 5 μM of each control primer specific for human c-myc promoter. Store at -20°C.
      FOR: CCC AAA AAA AGG CAC GGA A
      REV: TAT TGG AAA TGC GGT CAT GC
      Quality LevelMQ100
      Applications
      ApplicationUse ChIPAb+ LEF1 Mouse Monoclonal Antibody validated in WB, ChIP for the detection of LEF1.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blot Analysis:
      Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-LEF1 (1 mg/mL). Proteins were visualized using a goat antimouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
      Biological Information
      ImmunogenThe antibody is made against amino acid residues 1-85 of human LEF1.
      HostMouse
      SpecificityRecognizes LEF1 (lymphoid enhancer-binding factor 1).
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryLymphoid enhancer-binding factor-1 (LEF1) is a 48-kD nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905).[supplied by OMIM]
      Gene Symbol
      • LEF1
      • DKFZp586H0919
      • TCF1ALPHA
      • LEF-1
      • TCF1-alpha
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UJU2 # Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.
      SIZE: 399 amino acids; 44201 Da
      SUBUNIT: Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG (By similarity). Binds THOC4, MDFI and MDFIC.
      SUBCELLULAR LOCATION: Nucleus (By similarity). Note=Found in nuclear bodies upon PIASG binding (By similarity).
      TISSUE SPECIFICITY: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines.DOMAIN:SwissProt: Q9UJU2 Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.
      SIMILARITY: Belongs to the TCF/LEF family. & Contains 1 HMG box DNA-binding domain.
      Molecular Weight55-57 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceChromatin Immunoprecipitation:
      Sonicated chromatin prepared from 3x106 HT29 cells was subjected to chromatin immunoprecipitation using 4 μg of either the negative control antibody, Mouse Normal IgG or mouse Anti-LEF1 and the Magna ChIP™ G kit (Cat.# 17-611).
      Successful enrichment of LEF1 associated DNA fragments was verified by qPCR using ChIP Primers c-myc flanking the human c-myc promoter that contains LEF1 binding sites (Please see figures).
      Please refer to the EZ-Magna ChIP™ G (Cat.# 17-409) or EZ-ChIP™ (Cat.# 17-371) kit protocols for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C from date of shipment
      Packaging Information
      Material Size25 assays
      Material Package25 assays per kit. ~4μg per chromatin immunoprecipitation.
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalog Number GTIN
      17-604 04053252586439

      Documentation

      ChIPAb+ LEF1 - ChIP Validated Antibody and Primer Set SDS

      Title

      Safety Data Sheet (SDS) 

      ChIPAb+ LEF1 - ChIP Validated Antibody and Primer Set Certificates of Analysis

      TitleLot Number
      ChIPAb+ LEF1 - 1982644 1982644
      ChIPAb+ LEF1 - 2036867 2036867
      ChIPAb+ LEF1 - 2073182 2073182
      ChIPAb+ LEF1 - 2178414 2178414
      ChIPAb+ LEF1 - DAM1498544 DAM1498544
      ChIPAb+ LEF1 - DAM1658658 DAM1658658
      ChIPAb+ LEF1 - DAM1708255 DAM1708255
      ChIPAb+ LEF1 - DAM1761597 DAM1761597
      ChIPAb+ LEF1 - DAM1811165 DAM1811165
      ChIPAb+ LEF1 - JBC1872227 JBC1872227

      References

      Reference overviewPub Med ID
      Gbx2 directly restricts Otx2 expression to forebrain and midbrain, competing with class III POU factors.
      Inoue, F; Kurokawa, D; Takahashi, M; Aizawa, S
      Molecular and cellular biology  32  2618-27  2012

      Show Abstract
      22566684 22566684
      β-Catenin/LEF1 transactivates the microRNA-371-373 cluster that modulates the Wnt/β-catenin-signaling pathway.
      A-D Zhou,L-T Diao,H Xu,Z-D Xiao,J-H Li,H Zhou,L-H Qu
      Oncogene  31  2012

      Show Abstract
      22020335 22020335

      Brochure

      Title
      Advance your Epigenetics Research

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      Categories

      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Primary Antibodies
      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies