17-10260 ChIPAb+™ N-CoR Antibody

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17-10260
25 assays  25 assays per set. Recommended use: 3 µg of antibody per chromatin immuno-precipitation (dependent upon biological context).
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      H, M WB, ChIP
      Description
      Catalogue Number17-10260
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+™ N-CoR Antibody
      OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
      The ChIPAb+JMJD1C set includes the JMJD1C antibody, a Normal Rabbit IgG, and control primers which amplify a 110 bp region of ChIP Primers, human β-globin. The JMJD1C and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of JMJD1C -associated chromatin.
      Alternate Names
      • Nuclear receptor corepressor 1
      • N-CoR
      • N-CoR1
      • Retinoid X receptor-interacting protein 13
      Background InformationIn the absence of ligands, the corepressor N-CoR represses nuclear hormone receptor-mediated gene transcription. In the presence of ligands, these hormone receptors activate gene expression and play critical roles in development, growth, and homeostasis. N-Cor-mediated transcriptional repression is facilitated through the formation of histone deacetylase (HDACs) N-CoR complexes or by conjugation from small ubiquitin-like modifier 1 (SUMO-1). It is shown that the SANT1 domain of N-CoR serves as a binding region for SUMO-E2 conjugating enzyme Ubc9 and SUMO-E3 ligase Pias1, which is necessary for N-CoR-mediated repression of respective reporter genes. SUMOylation-deficient N-COR knockout mice display a signification reduction in the repression of gene transcription and result in defects involving the differentiation and development of neural cells into erythrocytes and thymocytes.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Includes normal rabbit IgG and primers specific for human p21.
      PresentationComponents:
      Anti-N-CoR (Rabbit Polyclonal). One vial containing 75 µg of affinity purified polyclonal in buffer containing 0.1M Tris-glycine (pH 7.4) and 150 mM NaCl with 0.05% sodium azide before the addition of 30% glycerol. Store at -20°C.
      Concentration: 0.7 mg/mL
      Normal Rabbit IgG One vial containing 125 µg of Rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.
      ChIP Primers, p21. One vial containing 75 μL of each primer (5 μM) specific for the human p21 (WAF1/CIP1/CDKN1A) promoter. Store at
      -20°C.
      FOR: CCC ACA GCA GAG GAG AAA GAA
      REV: CTG GAA ATC TCT GCC CAG ACA
      Quality LevelMQ100
      Applications
      ApplicationThis ChIPAb+ N-CoR -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      Representative lot data.
      Sonicated chromatin prepared from untreated or UV treated (6 hrs, 50 joules/m2.) U2OS cells (3 X 10E6 cell equivalents per IP) was subjected to chromatin immunoprecipitation using using 3 µg of either Normal Rabbit IgGor 3 µg of Anti-N-CoR and the Magna ChIP™ A/G Kit (Cat. # 17-10085). Successful immunoprecipitation of N-CoR associated DNA fragments was verified by qPCR using ChIP Primers, p21. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
      Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
      Western Blot Analysis:
      Representative lot data.
      RAW 264.7 cell lysate was probed with Anti-N-CoR (1 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
      Arrow indicates Anti-N-CoR (~270 kDa). (Figure 3).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a region between amino acids 1510 and 1540 of the N-CoR protein.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ncor1
      • Rxrip13
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Mediates transcriptional repression by certain nuclear receptors. Part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors.

      SUBUNIT STRUCTURE: Interacts with ZBTB33; the interaction serves to recruit the N-CoR complex to promoter regions containing methylated CpG dinucleotides. Interacts with TRIM28 and KDM3A. Interacts (via the RD1 domain) with BAZ1A (via its N-terminal); the interaction corepresses a number of NCOR1-regulated genes. Interacts with HEXIM1 By similarity. Interacts with C1D, SIAH2, HDAC7, SAP30, SIN3A and SIN3B. Forms a large corepressor complex that contains SIN3A/B and histone deacetylases HDAC1 and HDAC2. This complex associates with the thyroid receptor (TR) and the retinoid acid receptor (RAR) in the absence of ligand. Interacts directly with RARA; the interaction is facilitated with RARA trimethylation. Interacts with DACH1. Component of the N-Cor repressor complex, at least composed of CBFA2T3, HEXIM1, NCOR1, NCOR2, HDAC3, TBL1X, TBL1XR1, CORO2A and GPS2.

      SUBCELLULAR LOCATION: Nucleus.
      Molecular Weight~270 kDa observed. Uniprot describes 2 isoforms produced by alternative splicing at MW of 266 kDa and 270 kDa.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceChromatin Immunoprecipitation:
      Representative lot data.
      Sonicated chromatin prepared from U2OS cells (3 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 3 µg of either Normal Rabbit IgGor 3 µg of Anti-N-CoR and the Magna ChIP™ A/G Kit (Cat. # 17-10085). Successful immunoprecipitation of N-CoR associated DNA fragments was verified by qPCR using ChIP Primers, p21flanking an Sp1 binding site in the human p21 promoter. (Figure 1).
      Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size25 assays
      Material Package25 assays per set. Recommended use: 3 µg of antibody per chromatin immuno-precipitation (dependent upon biological context).
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      ChIPAb+™ N-CoR Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      ChIPAb+™ N-CoR Antibody Certificates of Analysis

      TitleLot Number
      ChIPAb+ N-CoR - 20648342064834
      ChIPAb+ N-CoR - 22721352272135
      ChIPAb+ N-CoR - Q1975301Q1975301
      ChIPAb+™ N-CoR - 32518683251868
      ChIPAb+™ N-CoR -25130092513009
      ChIPAb+™ N-CoR -26601702660170
      ChIPAb+™ N-CoR -27923662792366
      ChIPAb+™ N-CoR Polyclonal Antibody/Primer Set2859721
      ChIPAb+™ N-CoR Polyclonal Antibody/Primer Set3026470
      ChIPAb+™ N-CoR Polyclonal Antibody/Primer Set3131452

      References

      Reference overviewPub Med ID
      SUMOylation of the corepressor N-CoR modulates its capacity to repress transcription.
      Tiefenbach, Jens, et al.
      Mol. Biol. Cell, 17: 1643-51 (2006)  2006

      Show Abstract
      16421255 16421255
      A novel nuclear receptor corepressor complex, N-CoR, contains components of the mammalian SWI/SNF complex and the corepressor KAP-1.
      Underhill, C, et al.
      J. Biol. Chem., 275: 40463-70 (2000)  2000

      Show Abstract
      11013263 11013263
      The corepressor N-CoR and its variants RIP13a and RIP13Delta1 directly interact with the basal transcription factors TFIIB, TAFII32 and TAFII70.
      Muscat, G E, et al.
      Nucleic Acids Res., 26: 2899-907 (1998)  1998

      Show Abstract
      9611234 9611234

      Brochure

      Title
      Advance your Epigenetics Research (MilliporeSigma)

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Primary Antibodies
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies