17-10262 ChIPAb+™ JMJD1C - ChIP Validated Antibody and Primer Set

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17-10262
25 assays  25 assays per set. Recommended use: 1.4 µg (2 µL) of antibody per chromatin immuno-precipitation (dependent upon biological context).
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      H, Rb, Chp, Rhesus Macaque WB, ICC, ChIP
      Description
      Catalogue Number17-10262
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+™ JMJD1C - ChIP Validated Antibody and Primer Set
      OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
      The ChIPAb+JMJD1C set includes the JMJD1C antibody, a Normal Rabbit IgG, and control primers which amplify a 110 bp region of ChIP Primers, human β-globin. The JMJD1C and negative controls are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of JMJD1C -associated chromatin.
      Alternate Names
      • JMJD
      • jumonji domain containing 1C
      • thyroid receptor interacting protein 8
      • probable JmjC domain-containing histone demethylation protein 2C
      • thyroid hormone receptor interactor 8
      • Thyroid receptor-interacting protein 8
      • Jumonji domain-containing protein 1C
      • TR-interacting protein 8
      Background InformationJMJD1C (jumonji domain-containing protein 1C) is also known as TRIP8 and mutations in this histone demethylase have been linked to certain forms of autism. JMJD1C is also thought to work in reactivating genes in embryonic stem cells, pancreatic islet and gastric cancer. JMJD1C has also been implicated as a gene expression regulator. JMJD1C with WHISTLE have recently been shown to also play a role in mouse testis development. JMJD1C has been identified as a nuclear-receptor coactivator and is a candidate gene for autism.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Includes normal rabbit IgG and primers specific for human β-globin.
      PresentationAnti-JMJD1C (rabbit polyclonal). One vial containing 50 µL of purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide before the addition of glycerol to 30%. Store at -20°C.
      Concentration: 0.7 mg/mL
      Normal Rabbit IgG. One vial containing 125 µg of Rabbit IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.
      ChIP Primers, human β-globin. One vial containing 75 μL of each primer (5 μM) specific for the human β-globin
      promoter. Store at -20°C.
      FOR: AGG ACA GGT ACG GCT GTC ATC
      REV: TTT ATG CCC AGC CCT GGC TC
      Quality LevelMQ100
      Applications
      ApplicationThis ChIPAb+ JMJD1C -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      Representative lot data.
      Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1.4 µg of either Normal Rabbit IgG or Anti-JMJD1C and the Magna ChIP™ A Kit (Cat. # 17-610). Successful immunoprecipitation of JMJD1C associated DNA fragments was verified by qPCR using ChIP Primers, human β-globin as a positive locus, and β-actin promoter primers as a negative locus. (Figure 2). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
      Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
      Western Blot Analysis:
      Representative lot data.
      HeLa nuclear extract was probed with Anti-JMJD1C (1 μg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system.
      Arrow indicates JMJD1C (~284 kDa). (Figure 3).
      Immunocytochemistry Analysis:
      Representative lot data.
      Confocal fluorescent analysis of HeLa cells using Anti-JMJD1C (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye -Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus. (Figure 4).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to human JMJD1C.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      Species Reactivity
      • Human
      • Rabbit
      • Chimpanzee
      • Rhesus Macaque
      Species Reactivity NoteDemonstrated to react with human. Predicted to react with rabbit, chimpanzee, rhesus macaque, and orangutan based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TRIP8
      • RP11-10C13.2
      • JMJD1C
      • JHDM2C
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Probable histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Demethylation of Lys residue generates formaldehyde and succinate. May be involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes (By similarity).

      COFACTOR: Binds 1 Fe2+ ion per subunit (By similarity).

      SUBUNIT STRUCTURE: Interacts specifically with the ligand-binding domain of the thyroid receptor (TR). Requires the presence of thyroid hormone for its interaction.

      SUBCELLULAR LOCATION: Nucleus (By similarity).

      DOMAIN: Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors (By similarity).

      PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.

      SEQUENCE SIMILARITIES: Belongs to the JHDM2 histone demethylase family.

      Contains 1 JmjC domain.

      SEQUENCE CAUTION: The sequence AAC41741.1 differs from that shown. Reason: Frameshift at position 2171.

      The sequence CAD97921.1 differs from that shown. Reason: Frameshift at position 1697.

      The sequence CAI10947.1 differs from that shown. Reason: Erroneous gene model prediction.
      Molecular Weight~284 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceChromatin Immunoprecipitation:
      Representative lot data.
      Sonicated chromatin prepared from HeLa cells (1 X 10E6 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 1.4 µg of either Normal Rabbit IgG, or Anti-JMJD1C and the Magna ChIP™ A Kit (Cat. # 17-610).
      Successful immunoprecipitation of JMJD1C associated DNA fragments was verified by qPCR using ChIP Primers, human β-globin (Figure 1).
      Please refer to the EZ-Magna ChIP™ A (Cat. # 17-408) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size25 assays
      Material Package25 assays per set. Recommended use: 1.4 µg (2 µL) of antibody per chromatin immuno-precipitation (dependent upon biological context).
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      ChIPAb+™ JMJD1C - ChIP Validated Antibody and Primer Set SDS

      Title

      Safety Data Sheet (SDS) 

      ChIPAb+™ JMJD1C - ChIP Validated Antibody and Primer Set Certificates of Analysis

      TitleLot Number
      ChIPAb+ JMJD1C - 21236802123680
      ChIPAb+ JMJD1C - Q1957678Q1957678
      ChIPAb+™ JMJD1C - 31894123189412
      ChIPAb+™ JMJD1C - 32914203291420
      ChIPAb+™ JMJD1C - 33254773325477
      ChIPAb+™ JMJD1C - 34399643439964
      ChIPAb+™ JMJD1C - 34820503482050
      ChIPAb+™ JMJD1C - 35103193510319
      ChIPAb+™ JMJD1C -25859302585930
      ChIPAb+™ JMJD1C -28134822813482

      References

      Reference overviewPub Med ID
      Pluripotency transcription factor Oct4 mediates stepwise nucleosome demethylation and depletion.
      Shakya, A; Callister, C; Goren, A; Yosef, N; Garg, N; Khoddami, V; Nix, D; Regev, A; Tantin, D
      Molecular and cellular biology  35  1014-25  2015

      Show Abstract
      25582194 25582194

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      Title
      Advance your Epigenetics Research (MilliporeSigma)

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Primary Antibodies
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies