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17-603 ChIPAb+ Estrogen Receptor α - ChIP Validated Antibody and Primer Set

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17-603
25 assays  25 assays per kit. ~4 μL per chromatin immunoprecipitation.
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      H, MWB, ChIP
      Description
      Catalogue Number17-603
      Brand Family Upstate
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+ Estrogen Receptor α - ChIP Validated Antibody and Primer Set
      OverviewEvery lot of the ChIPAb+ line of antibodies is individually validated for chromatin precipitation, in order to guarantee successful ChIP assays every time. Each antibody includes a control primer set for performance confirmation. ERα antibody is functionally validated in the precipitation of ERα associated chromatin.
      The qPCR primers included flank the ERα binding site in human pS2 promoter.
      References
      Product Information
      FormatAscites
      Control
      • Includes control primers specific for human TFF1 (pS2) promoter.
      PresentationAnti-ERα (mouse ascites). 1 vial containing 100 mL ascites. Store at -20°C. The ERα antibody is made against aa120-170 of bovine estrogen a. It can recognize human and mouse ERα.

      ChIP primers TFF1 (pS2). 1 vial containing 75 mL of 5 μM of each control primer specific for human TFF1 (pS2) promoter. Store at -20°C.
      FOR: CCG GCC ATC TCT CAC TAT GAA
      REV: CCT TCC CGC CAG GGT AAA TAC
      Quality LevelMQ100
      Applications
      ApplicationThis ChIPAb+ Estrogen Receptor α -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      Sonicated chromatin, prepared from 3x106 MCF7 cells that are either estrogen starved or b-estradiol treated (100 nM, 45 min.) was subjected to chromatin
      immunoprecipitation using 4 mL anti-ERa and the Magna ChIP™ G (Cat.# 17-611) kit Standard Protocol. Successful enrichment of ER associated DNA fragments was verified by qPCR using ChIP Primers TFF1 (pS2) flanking the human TFF1 promoter that contains an ER binding site (Please see figures).
      Please refer to the EZ-Magna ChIP™ G (Cat.# 17-409) or EZ-ChIP™ (Cat.# 17-371) kit protocols for experimental details.

      Western Blot Analysis:
      MCF7cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-ERa (1:1000 dilution). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system (Please see figures).
      Biological Information
      ImmunogenThe ERα antibody is made against aa 120-170 of bovine estrogen α.
      HostMouse
      SpecificityEstrogen Receptor α
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in several transcript variants, which differ in their 5' UTRs and use different promoters.
      Gene Symbol
      • ESR1
      • Era
      • ER-alpha
      • ESR
      • ER
      • NR3A1
      • ESRA
      • DKFZp686N23123
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P03372 # Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
      SIZE: 595 amino acids; 66216 Da
      SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with SLC30A9 (By similarity). Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1.
      SUBCELLULAR LOCATION: Nucleus.
      DOMAIN: SwissProt: P03372 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
      PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity.
      SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain.
      MISCELLANEOUS: In the absence of ligand, steroid hormone receptors are thought to be weakly associated with nuclear components; hormone binding greatly increases receptor affinity. The hormone- receptor complex appears to recognize discrete DNA sequences upstream of transcriptional start sites.
      Molecular Weight66 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceChromatin immunoprecipitation:
      Sonicated chromatin prepared from 3x106 MCF7 cells that were treated with b-estradiol (100 nM, 45 min.) was subjected to chromatin immunoprecipitation using 4 mL anti-ERα and beads only control.
      Successful enrichment of ERα associated DNA fragments was verified by qPCR using ChIP Primers TFF1 (pS2) flanking the human TFF1 promoter that contains an ER binding site (Please see figures).
      Please refer to the EZ-Magna ChIP™ G (Cat.# 17- 409) or EZ-ChIP™ (Cat.# 17-371) kit protocols for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at -20°C from date of shipment
      Packaging Information
      Material Size25 assays
      Material Package25 assays per kit. ~4 μL per chromatin immunoprecipitation.
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      ChIPAb+ Estrogen Receptor α - ChIP Validated Antibody and Primer Set SDS

      Title

      Safety Data Sheet (SDS) 

      ChIPAb+ Estrogen Receptor α - ChIP Validated Antibody and Primer Set Certificates of Analysis

      TitleLot Number
      ChIPAb+ Estrogen Receptor - 2225263 2225263
      ChIPAb+ Estrogen Receptor #945; - 2113337 2113337
      ChIPAb+ Estrogen Receptor - R0708G0052 R0708G0052
      ChIPAb+ Estrogen Receptor a - 2155400 2155400
      ChIPAb+ Estrogen Receptor a - DAM1480392 DAM1480392
      ChIPAb+ Estrogen Receptor a - DAM1764569 DAM1764569
      ChIPAb+ Estrogen Receptor a - JBC1862628 JBC1862628
      ChIPAb+ Estrogen Receptor a - JBC1940026 JBC1940026
      ChIPAb+ Estrogen Receptor a - JBC1941197 JBC1941197
      ChIPAb+™ Estrogen Receptor α - 3185805 3185805

      Brochure

      Title
      Advance your Epigenetics Research (MilliporeSigma)

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      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Primary Antibodies
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies