Millipore Sigma Vibrant Logo

17-681 ChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set

View Products on Sigmaaldrich.com
17-681
25 assays  25 assays per kit, ~2μg per chromatin immunoprecipitation
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      VrtWB, ChIP
      Description
      Catalogue Number17-681
      Brand Family Upstate
      Trade Name
      • ChIPAb+
      • Upstate
      DescriptionChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set
      OverviewAll ChIPAb+ antibodies are individually validated for chromatin precipitation, every lot, every time. Each ChIPAb+ antibody set includes control primers (tested every lot by qPCR) to biologically validate your IP results in a locus-specific context. The qPCR protocol and primer sequences are provided, allowing researchers to validate ChIP protocols when using our antibody in their chromatin context. Each set also includes a negative control antibody to ensure specificity of the ChIP reaction.
      The ChIPAb+ Dimethyl-Histone H3 (Lys9) set includes the Anti-dimethyl-Histone H3 (Lys9) antibody, a negative control antibody (purified Mouse IgG), and qPCR primers which amplify a 110 bp region within the promoter of the human β-globin gene. The dimethyl-histone H3 (Lys9) and negative control antibodies are supplied in a scalable "per ChIP" reaction size and can be used to functionally validate the precipitation of dimethyl-histone H3 (Lys9) associated chromatin.
      Alternate Names
      • H3K9me2
      • Histone H3 (di methyl K9)
      References
      Product Information
      FormatPurified
      Control
      • Included negative control mouse IgG antibody and control primers specific for human β-globin promoter.
      PresentationAnti-dimethyl-Histone H3 (Lys9) (mouse monoclonal IgG1қ, Clone CMA307). One vial containing 50 μg of protein G purified antibody in 50 μL PBS containing 0.05% sodium. Store at -20°C.

      Normal Mouse IgG. Two vials containing 25 μg purified Mouse IgG in 25 μL storage buffer containing 0.1% sodium azide. Store at -20°C.

      ChIP Primers B-Globin. One vial containing 75 μL of 5 μM of each primer specific for human β-globin.
      Store at -20°C.
      FOR: AGG ACA GGT ACG GCT GTC ATC
      REV: TTT ATG CCC AGC CCT GGC TC
      Quality LevelMQ100
      Applications
      ApplicationThis ChIPAb+ Dimethyl-Histone H3 (Lys9) -ChIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesChromatin Immunoprecipitation:
      Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) was subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-dimethyl-Histone H3 (Lys9) antibody and the Magna ChIP G (Cat. #17-611) Kit. Successful immunoprecipitation of dimethyl-histone H3 (Lys9) associated DNA fragments was verified by qPCR using β-globin ChIP Primers versus Control Primers (Please see figures). Data is presented as percent input of each IP sample relative to input chromatin for each amplicon and ChIP sample as indicated.
      Please refer to the EZ-Magna G ChIP™ (Cat. #17-408) or EZ-ChIP™ (Cat. #17-371) protocol for experimental details.

      Dot Blot Analysis: Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Anti-dimethyl H3 (Lys9) at 2.0ug/mL (1:500) dilution. Proteins were visualized using a Donkey anti-mouse IgG conjugated to HRP and a chemiluminescence detection system.

      Western Blot Analysis:
      Recombinant Histone H3 (Lane 1) and HeLa acid extract (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with Anti-dimethyl Histone H3 (Lys9), clone CMA307 (0.1 μg/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

      Multiplexing:
      This antibody specifically recognizes histone H3 dimethylated on Lys9 by Luminex® assay.

      ELISA: This antibody has been shown by an outside laboratory to be suitable for ELISA.

      Immunocytochemistry: This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry.

      Immunoprecipitation: This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation.
      Biological Information
      ImmunogenThe dimethyl-histone H3 (Lys9) purified antibody is made against a synthetic peptide (dimethylated at Lys9) corresponding to amino acids 1-18 of histone H3.
      Epitopea.a. 1-18
      CloneCMA307
      HostMouse
      SpecificityRecognizes histone H3, Mr 17 kDa, dimethylated at lysine 9.
      IsotypeIgG
      Species Reactivity
      • Vertebrates
      Species Reactivity NoteHuman, although this peptide sequence is identical in a wide range of animal and plant species.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHistones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
      Modifications
      • Methylation
      UniProt Number
      UniProt SummaryFUNCTION:Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Ref.14 Ref.18 Ref.22

      SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with HIRA, a chaperone required for its incorporation into nucleosomes. Ref.14

      SUBCELLULAR LOCATION: Nucleus.

      Developmental stage Expressed throughout the cell cycle independently of DNA synthesis.

      PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18.

      Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription.

      Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression.

      Specifically enriched in modifications associated with active chromatin such as methylation at Lys-5, Lys-37 and Lys-80. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28, which are linked to gene repression, are underrepresented. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin.

      Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase, probably DAPK3. Phosphorylation at 'Ser-11' by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation on Ser-32 is specific to regions bordering centromeres in metaphase chromosomes. Ref.9 Ref.10 Ref.12 Ref.13 Ref.19 Ref.20 Ref.21 Ref.29

      Ubiquitinated By similarity.

      SIMILARITY: Belongs to the histone H3 family.

      SEQUENCE CAUTION: The sequence CAH73371.1 differs from that shown. Reason: Erroneous gene model prediction.

      Molecular WeightDimethyl-histone H3 at ~17 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceChromatin Immunoprecipitation:
      Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG or Anti-dimethyl-Histone H3 (Lys9) antibody and the Magna ChIP G (Cat. #17-611) Kit. Successful immunoprecipitation of dimethyl-histone H3 (Lys9) associated DNA fragments was verified by qPCR using ChIP Primers B-Globin (Please see figures).
      Please refer to the EZ-Magna G ChIP™ (Cat. #17-409) or EZ-ChIP™ (Cat. #17-371) protocol for experimental details.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Aliquot upon initial thaw, avoid freeze thaw cycles.
      Packaging Information
      Material Size25 assays
      Material Package25 assays per kit, ~2μg per chromatin immunoprecipitation
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      ChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set SDS

      Title

      Safety Data Sheet (SDS) 

      ChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set Certificates of Analysis

      TitleLot Number
      ChIPAb+ Dimethyl Histone H3 (Lys9) - 2383999 2383999
      ChIPAb+ Dimethyl Histone H3 (Lys9) - 2309330 2309330
      ChIPAb+ Dimethyl Histone H3 (Lys9) - 3285845 3285845
      ChIPAb+ Dimethyl Histone H3 (Lys9) - 3386032 3386032
      ChIPAb+ Dimethyl Histone H3 (Lys9) - 3406967 3406967
      ChIPAb+ Dimethyl Histone H3 (Lys9) - 3538976 3538976
      ChIPAb+ Dimethyl Histone H3 (Lys9) -2489082 2489082
      ChIPAb+ Dimethyl Histone H3 (Lys9) -2664350 2664350
      ChIPAb+ Dimethyl Histone H3 (Lys9) -2694495 2694495
      ChIPAb+ Dimethyl Histone H3 (Lys9) Monoclonal Antibody/ Primer Set 2945922

      References

      Reference overviewApplicationPub Med ID
      Genome-Wide Profiling of Histone Modifications (H3K9me2 and H4K12ac) and Gene Expression in Rust (Uromyces appendiculatus) Inoculated Common Bean (Phaseolus vulgaris L.).
      Ayyappan, V; Kalavacharla, V; Thimmapuram, J; Bhide, KP; Sripathi, VR; Smolinski, TG; Manoharan, M; Thurston, Y; Todd, A; Kingham, B
      PloS one  10  e0132176  2015

      Show Abstract
      26167691 26167691
      An integrative analysis of post-translational histone modifications in the marine diatom Phaeodactylum tricornutum.
      Veluchamy, A; Rastogi, A; Lin, X; Lombard, B; Murik, O; Thomas, Y; Dingli, F; Rivarola, M; Ott, S; Liu, X; Sun, Y; Rabinowicz, PD; McCarthy, J; Allen, AE; Loew, D; Bowler, C; Tirichine, L
      Genome biology  16  102  2015

      Show Abstract
      25990474 25990474
      Hyperosmotic priming of Arabidopsis seedlings establishes a long-term somatic memory accompanied by specific changes of the epigenome.
      Sani, E; Herzyk, P; Perrella, G; Colot, V; Amtmann, A
      Genome biology  14  R59  2013

      Show Abstract
      23767915 23767915
      DNA demethylation and histone H3K9 acetylation determine the active transcription of the NKG2D gene in human CD8+ T and NK cells.
      Fernández-Sánchez, A; Baragaño Raneros, A; Carvajal Palao, R; Sanz, AB; Ortiz, A; Ortega, F; Suárez-Álvarez, B; López-Larrea, C
      Epigenetics  8  66-78  2013

      Show Abstract
      23235109 23235109
      Profiling genome-wide chromatin methylation with engineered posttranslation apparatus within living cells.
      Wang, R; Islam, K; Liu, Y; Zheng, W; Tang, H; Lailler, N; Blum, G; Deng, H; Luo, M
      Journal of the American Chemical Society  135  1048-56  2013

      Show Abstract
      Immunoprecipitation23244065 23244065
      Protocol: Chromatin immunoprecipitation (ChIP) methodology to investigate histone modifications in two model diatom species.
      Lin, X; Tirichine, L; Bowler, C
      Plant methods  8  48  2012

      Show Abstract
      23217141 23217141
      The chromatin-binding protein HMGN1 regulates the expression of methyl CpG-binding protein 2 (MECP2) and affects the behavior of mice.
      Abuhatzira, L; Shamir, A; Schones, DE; Schäffer, AA; Bustin, M
      The Journal of biological chemistry  286  42051-62  2011

      Show Abstract
      22009741 22009741

      Brochure

      Title
      Advance your Epigenetics Research (MilliporeSigma)

      Related Products & Applications

      Related Products

      Catalog Number Description  
      17-609 ChIPAb+ Acetyl-Histone H3 (Lys9) Serum - ChIP Validated Antibody and Primer Set Show Pricing & Availability
      17-614 ChIPAb+ Trimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set, rabbit monoclonal Show Pricing & Availability
      17-643 ChIPAb+ Monomethyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set Show Pricing & Availability
      17-648 ChIPAb+ Dimethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set Show Pricing & Availability
      17-658 ChIPAb+ Acetyl-Histone H3 (Lys9) Purified - ChIP Validated Antibody and Primer Set Show Pricing & Availability
      17-675 ChIPAb+ Histone H3 (Unmodified Lys4) - ChIP Validated Antibody and Primer Set Show Pricing & Availability
      17-677 ChIPAb+ Dimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set Show Pricing & Availability
      17-678 ChIPAb+ Trimethyl-Histone H3 (Lys4) - ChIP Validated Antibody and Primer Set Show Pricing & Availability
      17-680 ChIPAb+ Monomethyl-Histone H3 (Lys9) - ChIP Validated Antibody and Primer Set Show Pricing & Availability
      17-683 ChIPAb+ Acetyl-Histone H3 (Lys27) - ChIP Validated Antibody and Primer Set Show Pricing & Availability

      Product Families

      Categories

      Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies
      Life Science Research > Antibodies and Assays > Primary Antibodies
      Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Validated Antibodies