218813 Caspase-2, Human, Recombinant, E. coli

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218813
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218813-100U
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      Description
      OverviewRecombinant, human caspase-2 expressed in E. coli as a single polypeptide chain. Subsequently undergoes spontaneous auto-processing to yield the native active enzyme. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. Cleavage sites are located at Asp316, Asp330, and possibly Asp152. MW: 12000 and 19000.
      Catalogue Number218813
      Brand Family Calbiochem®
      SynonymsICH-1, Nedd-2
      References
      ReferencesTalanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
      Wang, L., et al. 1994. Cell 78, 739.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 nmol of Ac-VDVAD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=218820">Cat. No. 218820</a>) per h at 37°C, pH 7.2.
      FormLyophilized solid
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥1500 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Caspase-2, Human, Recombinant, E. coli SDS

      Title

      Safety Data Sheet (SDS) 

      Caspase-2, Human, Recombinant, E. coli Certificates of Analysis

      TitleLot Number
      218813

      References

      Reference overview
      Talanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
      Wang, L., et al. 1994. Cell 78, 739.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-June-2008 RFH
      SynonymsICH-1, Nedd-2
      DescriptionRecombinant, human caspase-2 expressed in E. coli as a single polypeptide chain. Subsequently undergoes spontaneous auto-processing to yield the native active enzyme. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. Cleavage sites are located at Asp316, Asp330, and possibly Asp152. MW: 12000 and 19000.
      FormLyophilized solid
      Recommended reaction conditions50 mM HEPES, pH 7.2, 50 mM NaCl, 0.1% CHAPS (Cat. No. 220201), 10 mM EDTA, 5% glycerol, 10 mM DTT, and 200 µM peptide substrate. For analyzing caspase-2 activity, use 1 unit of enzyme per assay.
      Purity≥90% by SDS-PAGE
      Specific activity≥1500 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 nmol of Ac-VDVAD-pNA (Cat. No. 218820) per h at 37°C, pH 7.2.
      SolubilityPBS (0.1-1 unit/µl)
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 6 months at -70°C.
      Toxicity Standard Handling
      ReferencesTalanian, R.V., et al. 1997. J. Biol. Chem. 272, 9677.
      Wang, L., et al. 1994. Cell 78, 739.