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208773 Calpain Substrate IV, Cell-Permeable, Fluorogenic - Calbiochem

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208773-1MG
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      Description
      OverviewThe non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (kcat = 0.2 s-1, kcat/KM = 5000 M-1 s-1) when compared to the parental peptide (kcat = 0.17 s-1, kcat/KM = 680 M-1 s-1). Its suitability in detecting cellular Ca2+-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca2+ addition.
      Catalogue Number208773
      Brand Family Calbiochem®
      Synonyms(DABCYL)-TPLK~SPPPSPRE(EDANS)-RRRRRRR-NH₂
      References
      ReferencesBanoczi, Z., et al. 2008. Bioconjug. Chem. 19, 1375.
      Product Information
      FormLyophilized solid
      FormulationSupplied as a trifluoroacetate salt
      Hill FormulaC₁₂₇H₂₀₆N₅₀O₂₇S
      Chemical formulaC₁₂₇H₂₀₆N₅₀O₂₇S
      Hygroscopic Hygroscopic
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Emission max.
      Excitation max.
      Peptide Sequence(DABCYL)-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-Glu(EDANS)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH₂
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Calpain Substrate IV, Cell-Permeable, Fluorogenic - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Calpain Substrate IV, Cell-Permeable, Fluorogenic - Calbiochem Certificates of Analysis

      TitleLot Number
      208773

      References

      Reference overview
      Banoczi, Z., et al. 2008. Bioconjug. Chem. 19, 1375.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision23-July-2014 JSW
      Synonyms(DABCYL)-TPLK~SPPPSPRE(EDANS)-RRRRRRR-NH₂
      DescriptionThe non-permeant fluorogenic Calpain Substrate III (Cat. No. 208771) is made cell-permeable by a c-terminal heptaarginine extension via a Glu linkage. In addition to the expected improvement in permeability, the modified peptide also exhibits enhanced Calpain B sensitivity (kcat = 0.2 s-1, kcat/KM = 5000 M-1 s-1) when compared to the parental peptide (kcat = 0.17 s-1, kcat/KM = 680 M-1 s-1). Its suitability in detecting cellular Ca2+-dependent calpain activity is demonstrated both in intact Drosophila melanogaster S2 cells following ionomycin stimulation and in S2 lysates via direct Ca2+ addition.
      FormLyophilized solid
      FormulationSupplied as a trifluoroacetate salt
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₁₂₇H₂₀₆N₅₀O₂₇S
      Peptide Sequence(DABCYL)-Thr-Pro-Leu-Lys~Ser-Pro-Pro-Pro-Ser-Pro-Arg-Glu(EDANS)-Arg-Arg-Arg-Arg-Arg-Arg-Arg-NH₂
      Purity≥95% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage -20°C
      Hygroscopic
      Protect from light
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesBanoczi, Z., et al. 2008. Bioconjug. Chem. 19, 1375.